Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,863,695	A→G	intergenic (‑92/‑204)	glvC ← / → yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,863,695	0	A	G	100.0%	106.8 / NA	31	intergenic (‑92/‑204)	glvC/yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/15);  total (16/15)

GTGAACATCGCGCCGCCAAAGCGTTGAATTTGACTGAGCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGAACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGAT  >  NC_000913/3863563‑3863827                                                                                                                                     |                                                                                                                                     gTGAACATCGCGCCGCCAAAGCGTTGAATTTGACTGAGCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATgac                                                                                                                               <  2:515560/140‑1 (MQ=255) gTGAACATCGCGCCGCCAAAGCGTTGAATTTGACTGAGCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATgac                                                                                                                               <  1:402838/140‑1 (MQ=255)         cgcgCCGCCAAAGCGTTGAATTTGACTGAGCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAAt                                                                                                                       >  2:549097/1‑140 (MQ=255)           cgccgcCAAAGCGTTGAATTTGACTGAGCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATgg                                                                                                                     >  2:431586/1‑140 (MQ=255)                 cAAAGCGTTGAATTTGACTGAGCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCg                                                                                                               >  2:251009/1‑140 (MQ=255)                      cGTTGAATTTGACTGAGCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATc                                                                                                          >  2:319302/1‑140 (MQ=255)                                      gCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGTCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCg                                                                                          <  2:117396/140‑1 (MQ=255)                                         tttCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTg                                                                                       <  2:92881/140‑1 (MQ=255)                                           tCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTggg                                                                                     <  1:549097/140‑1 (MQ=255)                                               caTCCTCTTTTCTCAATTCTGTAAGTCGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGt                                                                                 <  1:431586/140‑1 (MQ=255)                                                    tctTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAgaga                                                                            >  2:312443/1‑140 (MQ=255)                                                    tctTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAgaga                                                                            >  1:449321/1‑140 (MQ=255)                                                        ttCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTa                                                                        >  1:115587/1‑140 (MQ=255)                                                                  tGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTcgcg                                                              >  1:441250/1‑140 (MQ=255)                                                                    tAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGACAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGaa                                                            >  2:550679/1‑140 (MQ=255)                                                                           gAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAg                                                     >  2:243847/1‑140 (MQ=255)                                                                             aGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGAc                                                   <  1:251009/140‑1 (MQ=255)                                                                              gCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGAcc                                                  <  1:240396/140‑1 (MQ=255)                                                                                 tGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACctct                                               <  2:526258/140‑1 (MQ=255)                                                                                   aTTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTTGTGTTCGCTTTCATGCTTTTCGTTGGGCGGTAGAGAGCTACGTTTTCGCGAAAAAGTAGACCTCtgt                                             >  2:255470/1‑140 (MQ=255)                                                                                      ggTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAg                                          <  1:312443/140‑1 (MQ=255)                                                                                      ggTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAg                                          <  1:243847/140‑1 (MQ=255)                                                                                        ttATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACctct                                               >  1:410903/1‑133 (MQ=255)                                                                                        ttATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACctct                                               <  2:410903/133‑1 (MQ=255)                                                                                           tGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAg                                     <  2:449321/140‑1 (MQ=255)                                                                                                cAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTaca                                <  2:115587/140‑1 (MQ=255)                                                                                                 aGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTacac                               >  1:151527/1‑140 (MQ=255)                                                                                                     cTGGAGCGAGGATAGGAAGATGCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCa                           <  1:319302/140‑1 (MQ=255)                                                                                                                          gCGATCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTaaaaaaa      >  2:503214/1‑140 (MQ=255)                                                                                                                             aTCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGa   >  1:26967/1‑140 (MQ=255)                                                                                                                              tCGGCAGGACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGAt  >  2:5432/1‑140 (MQ=255)                                                                                                                              tCGGCAGGACATGACGACCCAATGGGATTCGTGATAGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGAt  >  1:255104/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     GTGAACATCGCGCCGCCAAAGCGTTGAATTTGACTGAGCATTTCCACATCCTCTTTTCTCAATTCTGTAAGTAGGAAGCCTGATTGGTTATGCGGCAGGCCTGGAGCGAGGATAGGAAGATGCGATCGGCAGAACATGACGACCCAATGGGATTCGTGATCGCTTTCATGCTTTTCGTTGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGAT  >  NC_000913/3863563‑3863827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑