Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,879,282	T→C	T280A (ACT→GCT)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,879,282	0	T	C	100.0%	89.0 / NA	26	T280A (ACT→GCT)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/11);  total (15/11)

ACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATC  >  NC_000913/3879152‑3879415                                                                                                                                   |                                                                                                                                      aCGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTc                                                                                                                              <  1:210221/140‑1 (MQ=255) aCGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTc                                                                                                                              >  1:529682/1‑140 (MQ=255)           tCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGa                                                                                                                    <  2:529682/140‑1 (MQ=255)                   tCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATgttgtt                                                                                                            >  2:275130/1‑140 (MQ=255)                    cACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGttgttg                                                                                                           >  2:519032/1‑140 (MQ=255)                      cgcgCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGt                                                                                                         <  1:519032/140‑1 (MQ=255)                            tCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCa                                                                                                   >  2:482770/1‑140 (MQ=255)                                 aCCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGa                                                                                              >  1:429656/1‑140 (MQ=255)                                                        ttttGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTc                                                                       >  2:222182/1‑140 (MQ=255)                                                                         ttGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTc                                                      <  2:429656/140‑1 (MQ=255)                                                                              cATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGc                                                 >  2:187220/1‑140 (MQ=255)                                                                                 gTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCCGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTc                                                      <  1:438800/132‑1 (MQ=255)                                                                                 gTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCCGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTc                                                      >  2:438800/1‑132 (MQ=255)                                                                                      cGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCg                                         >  1:599952/1‑140 (MQ=255)                                                                                          cAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCtt                                     >  2:49325/1‑140 (MQ=255)                                                                                                    gTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGga                           <  1:187220/140‑1 (MQ=255)                                                                                                    gTCATCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGga                           <  2:599952/140‑1 (MQ=255)                                                                                                       aTCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgag                          <  1:510179/138‑1 (MQ=255)                                                                                                       aTCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgag                          >  2:510179/1‑138 (MQ=255)                                                                                                       aTCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGga                           >  1:91896/1‑137 (MQ=255)                                                                                                       aTCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGga                           <  2:91896/137‑1 (MQ=255)                                                                                                       aTCGCCGCACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGaa                        >  2:315475/1‑140 (MQ=255)                                                                                                              cACGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTagaag                 >  1:432131/1‑140 (MQ=255)                                                                                                                cGGAAGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGat               <  1:334764/140‑1 (MQ=255)                                                                                                                   aaGCCTGCCAGGTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATAtt            <  1:315475/140‑1 (MQ=255)                                                                                                                             ggTGAGCACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATc  >  1:353944/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                      ACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATC  >  NC_000913/3879152‑3879415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑