Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,886,269	C→T	G398G (GGC→GGT)	yidC →	membrane protein insertase YidC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,886,269	0	C	T	100.0%	172.2 / NA	52	G398G (GGC→GGT)	yidC	membrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/21);  total (31/21)

AACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGG  >  NC_000913/3886132‑3886400                                                                                                                                          |                                                                                                                                    aaCTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGa                                                                                                                                   >  2:88392/1‑140 (MQ=255)     ggggCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGAc                                                                                                                               <  1:46978/140‑1 (MQ=255)           tctcCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAg                                                                                                                         >  2:470004/1‑140 (MQ=255)                aTTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTAt                                                                                                                    <  1:280493/140‑1 (MQ=255)                      atcatcaCCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCa                                                                                                              >  1:330843/1‑140 (MQ=255)                        catcaCCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAgg                                                                                                            >  2:166318/1‑140 (MQ=255)                         atcaCCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTAt                                                                                                                    <  1:246817/131‑1 (MQ=255)                         atcaCCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTAt                                                                                                                    >  2:246817/1‑131 (MQ=255)                            aCCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAat                                                                                                        >  1:313849/1‑140 (MQ=255)                              cTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAatga                                                                                                      <  2:551270/140‑1 (MQ=255)                                ttATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAatgatg                                                                                                    <  2:313849/140‑1 (MQ=255)                                   tcgttcgtGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGgcg                                                                                                 <  2:181873/140‑1 (MQ=255)                                        cgtGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGgcg                                                                                                 <  1:348633/135‑1 (MQ=255)                                        cgtGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGgcg                                                                                                 >  2:348633/1‑135 (MQ=255)                                        cgtGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTa                                                                                            >  1:316062/1‑140 (MQ=255)                                        cgtGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTa                                                                                            >  2:86945/1‑140 (MQ=255)                                          tGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACa                                                                                          >  2:328559/1‑140 (MQ=255)                                           ggCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGGGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACaa                                                                                         >  2:364185/1‑140 (MQ=255)                                           ggCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACaa                                                                                         >  1:322964/1‑140 (MQ=255)                                                  tGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTgag                                                                                  >  1:143061/1‑140 (MQ=255)                                                  tGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATGCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTgag                                                                                  >  2:421349/1‑140 (MQ=255)                                                       ccGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGt                                                                             >  1:81750/1‑140 (MQ=255)                                                         gCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTa                                                                           >  2:340351/1‑140 (MQ=255)                                                          cTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTaa                                                                          >  1:90247/1‑140 (MQ=255)                                                          cTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTaa                                                                          >  1:105531/1‑140 (MQ=255)                                                                      cAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGggcgg                                                              <  2:330843/140‑1 (MQ=255)                                                                       aGTACACCTCCATGGCGAAGATGCGTATGTTGCGGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGggcggc                                                             >  1:484337/1‑140 (MQ=255)                                                                       aGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGggcggc                                                             >  1:546088/1‑140 (MQ=255)                                                                           cacCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGgctgct                                                         <  1:57002/140‑1 (MQ=255)                                                                              cTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTcc                                                      <  1:421349/140‑1 (MQ=255)                                                                                 cATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGgctg                                                           <  1:583855/132‑1 (MQ=255)                                                                                 cATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGgctg                                                           >  2:583855/1‑132 (MQ=255)                                                                                 cATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCgc                                                   >  1:502699/1‑140 (MQ=255)                                                                                   tGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCgctg                                                 >  2:322535/1‑140 (MQ=255)                                                                                    ggCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCgctgc                                                <  1:322535/140‑1 (MQ=255)                                                                                        aaGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGgctgct                                                         >  1:131680/1‑127 (MQ=255)                                                                                        aaGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGgctgct                                                         <  2:131680/127‑1 (MQ=255)                                                                                          gATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATcc                                          >  2:153524/1‑140 (MQ=255)                                                                                           aTGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCa                                         >  1:180644/1‑140 (MQ=255)                                                                                                  tGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCa                                  <  1:153524/140‑1 (MQ=255)                                                                                                       cAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCtt                             >  1:537113/1‑140 (MQ=255)                                                                                                         gCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTcc                           <  2:546088/140‑1 (MQ=255)                                                                                                            gAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTgg                        <  2:484337/140‑1 (MQ=255)                                                                                                            gAAGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTgg                        <  2:537113/140‑1 (MQ=255)                                                                                                              aGATTCAGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCg                      <  1:86945/140‑1 (MQ=255)                                                                                                                    aGGCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGtac                >  2:454325/1‑140 (MQ=255)                                                                                                                      gCAATGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCCGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGtacta              <  1:364185/140‑1 (MQ=255)                                                                                                                          tGCGTGAGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATg          >  1:397869/1‑140 (MQ=255)                                                                                                                                aGCGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATg    >  2:8562/1‑140 (MQ=255)                                                                                                                                  cGTCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATggg  <  2:143061/140‑1 (MQ=255)                                                                                                                                    tCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAGGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGta                 >  1:377098/1‑123 (MQ=255)                                                                                                                                    tCTGGGTGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAGGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGta                 <  2:377098/123‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    AACTGGGGCTTCTCCATTATCATCATCACCTTTATCGTTCGTGGCATCATGTACCCGCTGACCAAAGCGCAGTACACCTCCATGGCGAAGATGCGTATGTTGCAGCCGAAGATTCAGGCAATGCGTGAGCGTCTGGGCGATGACAAACAGCGTATCAGCCAGGAAATGATGGCGCTGTACAAAGCTGAGAAGGTTAACCCGCTGGGCGGCTGCTTCCCGCTGCTGATCCAGATGCCAATCTTCCTGGCGTTGTACTACATGCTGATGGG  >  NC_000913/3886132‑3886400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑