Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,241,586	A→G	S223S (AGT→AGC)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,586	0	A	G	100.0%	132.6 / NA	38	S223S (AGT→AGC)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/23);  total (15/23)

CCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGT  >  NC_000913/4241455‑4241719                                                                                                                                    |                                                                                                                                      ccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTgg                                                                                                                               <  1:551479/140‑1 (MQ=255)   ggTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGTGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCa                                                                                                                             <  2:241788/140‑1 (MQ=255)   ggTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCGGCGAGGGCTTTCTGGCa                                                                                                                             <  1:386979/140‑1 (MQ=255)   ggTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCa                                                                                                                             <  2:388593/140‑1 (MQ=255)        tGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGta                                                                                                                        >  2:534301/1‑140 (MQ=255)        tGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGta                                                                                                                        >  2:472092/1‑140 (MQ=255)            gCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATAca                                                                                                                    <  2:26626/140‑1 (MQ=255)             ccATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATAcag                                                                                                                   <  1:1350/140‑1 (MQ=255)                            tgtTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAAcag                                                                                                    <  2:72500/140‑1 (MQ=255)                            tgtTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAAcag                                                                                                    <  1:273655/140‑1 (MQ=255)                            tgtTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAAcag                                                                                                    >  2:311755/1‑140 (MQ=255)                             gtTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAAcagc                                                                                                   >  2:344360/1‑140 (MQ=255)                             gtTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCCGGCACGGTATACAGCAGCATTAAGAAcagc                                                                                                   >  2:201734/1‑140 (MQ=255)                              tttAATTTCCTGTACTGCCTGAGTTGCGAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAAcag                                                                                                    >  2:506407/1‑138 (MQ=255)                              tttAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAAcagca                                                                                                  >  1:376807/1‑140 (MQ=255)                              tttAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAAcag                                                                                                    <  1:506407/138‑1 (MQ=255)                                  aTTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGc                                                                                              <  1:425284/140‑1 (MQ=255)                                               ccTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTcc                                                                                 >  1:383627/1‑140 (MQ=255)                                                 tGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGa                                                                               >  2:78982/1‑140 (MQ=255)                                                 tGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGa                                                                               <  2:481071/140‑1 (MQ=255)                                                         aaGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACa                                                                       >  2:435241/1‑140 (MQ=255)                                                                   cccATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCa                                                             <  2:376807/140‑1 (MQ=255)                                                                   cccATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCa                                                             <  1:311755/140‑1 (MQ=255)                                                                     cATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGc                                                           <  1:78982/140‑1 (MQ=255)                                                                        aaTTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGt                                                        <  1:534301/140‑1 (MQ=255)                                                                        aaTTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGt                                                        <  1:472092/140‑1 (MQ=255)                                                                                   ggATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTcagc                                             <  1:570704/140‑1 (MQ=255)                                                                                              gCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATc                                  >  2:253307/1‑140 (MQ=255)                                                                                              gCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATc                                  >  2:416095/1‑140 (MQ=255)                                                                                               ccTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCa                                 <  1:220448/140‑1 (MQ=255)                                                                                                   ttCTTGCTTGCCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCgg                             >  2:588842/1‑140 (MQ=255)                                                                                                       tgcttgcCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACg                         <  2:51441/140‑1 (MQ=255)                                                                                                       tgcttgcCGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACg                         <  1:435241/140‑1 (MQ=255)                                                                                                             ccGCGCGACATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAAt                   >  1:7909/1‑140 (MQ=255)                                                                                                                    aCATCAGCCAGCGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAg            >  2:241533/1‑140 (MQ=255)                                                                                                                        cagccagcGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTa        <  2:383627/140‑1 (MQ=255)                                                                                                                        cagccagcGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTa        <  1:416095/140‑1 (MQ=255)                                                                                                                              gcGAGGGCTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGt  <  1:241533/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      CCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGT  >  NC_000913/4241455‑4241719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑