Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,376,552	T→C	intergenic (+110/‑1)	ecnA → / → ecnB	entericidin A lipoprotein, antidote to entericidin B/bacteriolytic entericidin B lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,376,552	0	T	C	100.0%	110.6 / NA	31	intergenic (+110/‑1)	ecnA/ecnB	entericidin A lipoprotein, antidote to entericidin B/bacteriolytic entericidin B lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

ATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGA  >  NC_000913/4376422‑4376685                                                                                                                                   |                                                                                                                                      aTCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTgaag                                                                                                                              <  1:296370/140‑1 (MQ=255)     ctcGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAagaaga                                                                                                                          <  1:173399/140‑1 (MQ=255)       cgcgCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACTTCATGGTGAAGAAGACa                                                                                                                        >  2:416623/1‑140 (MQ=255)          gcTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAAtt                                                                                                                     >  2:364603/1‑140 (MQ=255)               cAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGc                                                                                                                <  1:53375/140‑1 (MQ=255)                  cTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGAt                                                                                                             <  1:224032/140‑1 (MQ=255)                          tcttctcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTttctg                                                                                                     <  2:354389/140‑1 (MQ=255)                               tcttcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTttctgttctg                                                                                                <  1:364603/140‑1 (MQ=255)                               tattcCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACAATACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTttctgttctg                                                                                                <  2:528805/138‑1 (MQ=255)                                      aaaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCttt                                                                                         >  1:254201/1‑140 (MQ=255)                                      aaaaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCttt                                                                                         <  2:383683/140‑1 (MQ=255)                                        aaaTCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCa                                                                                       <  2:254201/140‑1 (MQ=255)                                                            tttttGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCaa                                                                   <  2:328516/140‑1 (MQ=255)                                                                   cGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACTTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCAcg                                                            <  1:416623/140‑1 (MQ=255)                                                                                         gCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACAttt                                      >  2:54544/1‑140 (MQ=255)                                                                                          cTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACa                                         <  1:586613/136‑1 (MQ=255)                                                                                          cTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACa                                         >  2:586613/1‑136 (MQ=255)                                                                                          cTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTc                                     >  1:209501/1‑140 (MQ=255)                                                                                            ataGGCAAACATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTg                                   >  1:475443/1‑140 (MQ=255)                                                                                                    aCATAAATAACATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGt                           >  1:397396/1‑140 (MQ=255)                                                                                                        aaataaCATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAAcg                       <  2:372228/140‑1 (MQ=255)                                                                                                          ataaCATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAAcgcg                     >  1:492378/1‑140 (MQ=255)                                                                                                            aaCATTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGAt                   <  2:475443/140‑1 (MQ=255)                                                                                                               aTTACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTc                >  1:338616/1‑140 (MQ=255)                                                                                                                ttACCTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCt               >  1:128058/1‑140 (MQ=255)                                                                                                                   ccTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGgc            <  1:364483/140‑1 (MQ=255)                                                                                                                   ccTAAAAGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTCGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGgc            <  2:499206/140‑1 (MQ=255)                                                                                                                         aGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCgcagca      >  1:533223/1‑140 (MQ=255)                                                                                                                         aGGAAGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCgcagca      >  1:599342/1‑140 (MQ=255)                                                                                                                            aaGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACg   >  1:331335/1‑140 (MQ=255)                                                                                                                             aGACGTCATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGa  <  1:33561/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      ATCTCTCGCGCTGCCAGCTAATTTTTCTTCTCTTCCGAAAAATCATCAGATTCCCATCATTTTTGGCGATGTTGTCTATTATTAATTTGCTATAGGCAAACATAAATAACATTACCTAAAAGGAAGACGTTATGGTGAAGAAGACAATTGCAGCGATCTTTTCTGTTCTGGTGCTTTCAACAGTATTAACTGCCTGCAACACCACGCGTGGCGTTGGTGAAGACATTTCTGATGGCGGTAACGCGATTTCTGGCGCAGCAACGA  >  NC_000913/4376422‑4376685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑