Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,094,703	(C)5→4	coding (378/981 nt)	yggR ←	Type II/IV secretion system family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,094,699	0	C	.	100.0%	171.4 / NA	41	coding (382/981 nt)	yggR	Type II/IV secretion system family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (27/14);  total (27/14)

CGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGC  >  NC_000913/3094563‑3094831                                                                                                                                           |                                                                                                                                     cGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGcccc                                                                                                                                   <  1:30463/140‑1 (MQ=255)       tgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGccccg                                                                                                                                  <  2:478333/135‑2 (MQ=255)       tgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGccccg                                                                                                                                  >  1:478333/1‑134 (MQ=255)           tCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGaa                                                                                                                        <  1:20311/140‑1 (MQ=255)                 aTCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGc                                                                                                                  <  2:93654/140‑1 (MQ=255)                    gctggctgGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCAt                                                                                                               >  2:497436/1‑140 (MQ=255)                    gctggctgGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCAt                                                                                                               >  1:509209/1‑140 (MQ=255)                      tggctggcATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATtc                                                                                                             >  1:44845/1‑140 (MQ=255)                             cATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGctct                                                                                                      >  1:689717/1‑140 (MQ=255)                               tAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTg                                                                                                    <  2:456987/140‑1 (MQ=255)                                    atatTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTaa                                                                                               >  2:569805/1‑140 (MQ=255)                                      atTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAAtt                                                                                             >  1:734364/1‑140 (MQ=255)                                       ttCAACAGGATCTTCCAGCGTCAGAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAAt                                                                                              <  2:418442/140‑1 (MQ=255)                                       ttCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATtct                                                                                                            <  1:470168/124‑1 (MQ=255)                                       ttCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATtct                                                                                                            >  2:470168/1‑124 (MQ=255)                                           aCAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGc                                                                                        >  2:470187/1‑140 (MQ=255)                                           aCAGAATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGc                                                                                        <  2:615922/140‑1 (MQ=255)                                            cAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCa                                                                                       >  2:414544/1‑140 (MQ=255)                                              ggATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAAt                                                                                     >  2:813922/1‑140 (MQ=255)                                               gATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATa                                                                                    >  1:824897/1‑140 (MQ=255)                                                    tCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGtt                                                                               >  2:789693/1‑140 (MQ=255)                                                       aGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGt                                                                            <  1:497436/140‑1 (MQ=255)                                                         cGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                              >  2:405570/1‑136 (MQ=255)                                                         cGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                              <  1:405570/136‑1 (MQ=255)                                                         cGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTgc                                                                          >  2:758928/1‑140 (MQ=255)                                                            c‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGcc                                                                       >  2:499366/1‑140 (MQ=255)                                                                gAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCaa                                                                     <  1:470187/140‑1 (MQ=255)                                                                  atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGc                                                                   <  1:58854/140‑1 (MQ=255)                                                                       cgcATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTc                                                              <  1:468138/140‑1 (MQ=255)                                                                                        tgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCg                                             <  1:758928/140‑1 (MQ=255)                                                                                        tgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCg                                             >  2:453694/1‑140 (MQ=255)                                                                                         gagaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                            >  1:617445/1‑140 (MQ=255)                                                                                                ccAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACGAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAAc                                     >  1:641460/1‑140 (MQ=255)                                                                                                ccAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAAc                                     >  2:589683/1‑140 (MQ=255)                                                                                                ccAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAAc                                     >  2:191518/1‑140 (MQ=255)                                                                                                ccAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAAc                                     >  2:57318/1‑140 (MQ=255)                                                                                                    ccATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGcc                                 >  1:174648/1‑140 (MQ=255)                                                                                                        cgccgcCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTATTTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTaa                             <  2:689717/140‑1 (MQ=255)                                                                                                          ccgccAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACg                           >  2:116010/1‑140 (MQ=255)                                                                                                                 cGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGa                    <  1:414544/140‑1 (MQ=255)                                                                                                                        gATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcca             >  1:337771/1‑140 (MQ=255)                                                                                                                             gCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCg        <  2:44845/140‑1 (MQ=255)                                                                                                                                  tCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTgcg   >  1:42321/1‑140 (MQ=255)                                                                                                                                   ccccGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTgcgc  >  2:559037/1‑140 (MQ=255)                                                                                                                                   ccccGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTgcgc  >  1:63829/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                     CGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTC‑‑AGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGC  >  NC_000913/3094563‑3094831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑