| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,257,356 | (T)5→4 | coding (655/1332 nt) | cyuP ← | putative D/L‑serine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,257,352 | 0 | T | . | 98.3% | 240.2 / ‑1.7 | 59 | coding (659/1332 nt) | cyuP | putative D/L‑serine transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (36/22); total (36/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TATTCATTGCCCGCAATGCTTTATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTG > NC_000913/3257218‑3257477 | tatTCATTGCCCGCAATGCTTTATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCGttttca < 1:12225/140‑3 (MQ=255) ttGCCCGCAATGCTTTATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACcagca > 1:785743/1‑140 (MQ=255) ccGCAATGCTTTATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGccc > 2:813678/1‑140 (MQ=255) tGCTTTATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAgcgg < 2:520516/140‑1 (MQ=255) tttATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGcggcgg > 1:150507/1‑140 (MQ=255) tATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTa < 1:813678/140‑1 (MQ=255) tATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTa < 2:445480/140‑1 (MQ=255) ccGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGa > 1:587460/1‑140 (MQ=255) gcgcCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAAc < 2:587460/140‑1 (MQ=255) gcgcCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAAc < 1:410640/140‑1 (MQ=255) cgcCACTTCTATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAAcc > 1:450912/1‑140 (MQ=255) cgcCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAAcc > 2:607235/1‑140 (MQ=255) cgcCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAAcc > 2:98193/1‑140 (MQ=255) cgcCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAAcc > 1:725641/1‑140 (MQ=255) ttCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGt < 1:149945/140‑1 (MQ=255) ttttCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAg > 1:784882/1‑136 (MQ=255) ttttCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAg < 2:784882/136‑1 (MQ=255) tCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGcc > 1:600343/1‑138 (MQ=255) tCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGcc < 2:600343/138‑1 (MQ=255) gagaTCACCACCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGac < 1:627599/140‑1 (MQ=255) aTCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATACCACATTCCCACCATCGACACg > 1:647591/1‑140 (MQ=255) cACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGcc < 1:607235/140‑1 (MQ=255) cACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGcc > 1:91538/1‑140 (MQ=255) cACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGcc > 1:798144/1‑140 (MQ=255) aCCATCNGANNNAANGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGccc > 2:889341/1‑140 (MQ=255) aCCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGccc < 2:725641/140‑1 (MQ=255) ccATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCa > 1:351364/1‑140 (MQ=255) ccATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCa > 2:499351/1‑140 (MQ=255) cATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAg > 2:679151/1‑140 (MQ=255) aTCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAgc > 1:30674/1‑140 (MQ=255) cGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAgcgc > 2:108176/1‑140 (MQ=255) gACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGcgccg < 2:250140/140‑1 (MQ=255) aCTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGcgccgc > 1:608785/1‑140 (MQ=255) ttAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGa < 2:608785/140‑1 (MQ=255) tAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGac < 2:798144/140‑1 (MQ=255) aaCGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGacc > 1:93070/1‑140 (MQ=255) aaCGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGac > 1:872556/1‑139 (MQ=255) aaCGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGac < 2:872556/139‑1 (MQ=255) tGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACcagc > 2:462034/1‑140 (MQ=255) gATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACcagcag > 1:571193/1‑140 (MQ=255) aTAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCagcagc < 2:30674/140‑1 (MQ=255) aTAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCagcagc < 1:462034/140‑1 (MQ=255) aTAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCagcagc < 1:531771/140‑1 (MQ=255) aTAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCagcagc < 1:552241/140‑1 (MQ=255) aCAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTgg > 1:29385/1‑140 (MQ=255) cAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGt < 1:98193/140‑1 (MQ=255) aTCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGc > 1:586272/1‑140 (MQ=255) aTCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGc > 2:824522/1‑140 (MQ=255) aTCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGc > 1:216164/1‑140 (MQ=255) aTCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGc > 1:701950/1‑140 (MQ=255) tCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCa > 1:111480/1‑140 (MQ=255) tCGACGTCAGGGTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCa > 2:772651/1‑140 (MQ=255) gTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTc < 2:647591/140‑1 (MQ=255) gTAAACGGCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTc > 2:891976/1‑140 (MQ=255) ggCAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAtt < 1:108176/140‑1 (MQ=255) cAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAtttt < 1:891976/140‑1 (MQ=255) cAGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAtttt > 2:853461/1‑140 (MQ=255) aGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTg > 1:845045/1‑140 (MQ=255) aGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTg > 1:760033/1‑140 (MQ=255) aGCGTAATAATGGCG‑TTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTg > 1:211878/1‑140 (MQ=255) | TATTCATTGCCCGCAATGCTTTATGCCGCGCCACTTCAATTGATTTTTCCCGCGAGCGATAAGAGATCACCATCGGACTTAACGTCTGGATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTG > NC_000913/3257218‑3257477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |