Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	705,972	(T)9→8	intergenic (+82/‑121)	nagE → / → glnS	N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	705,964	0	T	.	100.0%	110.7 / NA	27	intergenic (+74/‑129)	nagE/glnS	N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/11);  total (16/11)

AGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTG  >  NC_000913/705836‑706099                                                                                                                                 |                                                                                                                                        aGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCttttttttcatc                                                                                                                              <  1:475876/140‑5 (MQ=255)                     cGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTg                                                                                                         <  1:534388/140‑1 (MQ=255)                                       aTGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaa                                                                                       <  2:567790/140‑1 (MQ=255)                                                aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAAc                                                                              <  1:64700/140‑1 (MQ=255)                                                       aTCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTc                                                                       >  1:143226/1‑140 (MQ=255)                                                                    cTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCt                                                          >  2:897564/1‑140 (MQ=255)                                                                      gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTcc                                                              >  1:286428/1‑134 (MQ=255)                                                                      gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTcc                                                              <  2:286428/134‑1 (MQ=255)                                                                      gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTc                                                               <  2:310361/133‑1 (MQ=255)                                                                      gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTc                                                               >  1:310361/1‑133 (MQ=255)                                                                            aCGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGAt                                                  >  1:515090/1‑140 (MQ=255)                                                                             cGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTcc                                                              >  1:777020/1‑127 (MQ=255)                                                                             cGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTcc                                                              <  2:777020/127‑1 (MQ=255)                                                                              gCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCa                                                <  1:897564/140‑1 (MQ=255)                                                                                  gAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACg                                            <  2:841557/140‑1 (MQ=255)                                                                                                    tGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTAc                          >  1:351053/1‑140 (MQ=255)                                                                                                    tGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTAc                          >  2:58262/1‑140 (MQ=255)                                                                                                    tGACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTAc                          >  2:594720/1‑140 (MQ=255)                                                                                                     gACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACg                         >  1:745070/1‑140 (MQ=255)                                                                                                     gACAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACg                         >  1:737821/1‑140 (MQ=255)                                                                                                       cAAGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCt                       >  2:702418/1‑140 (MQ=255)                                                                                                         aGCGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCttt                     >  2:257698/1‑140 (MQ=255)                                                                                                           cGGTGGAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGa                   >  1:746668/1‑140 (MQ=255)                                                                                                               ggAGATCTTCTCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGaa               <  2:746668/140‑1 (MQ=255)                                                                                                                      ttctCTGCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGa        >  1:672948/1‑140 (MQ=255)                                                                                                                            gCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtgagtg  <  2:515090/140‑1 (MQ=255)                                                                                                                            gCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtgagtg  <  1:365807/140‑1 (MQ=255)                                                                                                                            gCCGC‑TTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGAtgagtg  >  2:208874/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                        AGCTCAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACGATGAGTG  >  NC_000913/705836‑706099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑