Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,528	(T)8→7	coding (887/2685 nt)	kdpD ←	sensory histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,521	0	T	.	100.0%	171.7 / NA	41	coding (894/2685 nt)	kdpD	sensory histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/24);  total (17/24)

ATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAA  >  NC_000913/723396‑723654                                                                                                                              |                                                                                                                                      atCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAg                                                                                                                        <  1:127783/140‑1 (MQ=255) atCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAg                                                                                                                        <  1:507933/140‑1 (MQ=255) atCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAg                                                                                                                        <  1:500759/140‑1 (MQ=255)       ccGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAg                                                                                                                        <  2:654059/134‑1 (MQ=255)       ccGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAg                                                                                                                        >  1:654059/1‑134 (MQ=255)            aTTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagggc                                                                                                             >  2:818559/1‑140 (MQ=255)             ttATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagggca                                                                                                            <  2:535017/140‑1 (MQ=255)             ttATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagggca                                                                                                            <  2:184614/140‑1 (MQ=255)             ttATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagggca                                                                                                            <  2:535018/140‑1 (MQ=255)             ttATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagggca                                                                                                            <  2:271292/140‑1 (MQ=255)             ttATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagggca                                                                                                            <  1:432165/140‑1 (MQ=255)                 gTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAgggg                                                                                                        >  2:4753/1‑140 (MQ=255)                 gTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAgggg                                                                                                        <  1:172849/140‑1 (MQ=255)                      cGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAAACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCa                                                                                                   >  2:230228/1‑140 (MQ=255)                          cATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACAt                                                                                               >  1:836909/1‑140 (MQ=255)                            tAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagg                                                                                                               <  2:401984/122‑1 (MQ=255)                            tAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagg                                                                                                               >  1:401984/1‑122 (MQ=255)                            tAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagg                                                                                                               <  2:132826/122‑1 (MQ=255)                            tAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTgcagg                                                                                                               >  1:132826/1‑122 (MQ=255)                               cGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGttt                                                                                                     <  2:526858/129‑1 (MQ=255)                               cGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGttt                                                                                                     >  1:526858/1‑129 (MQ=255)                                   cTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGt                                                                                      >  2:359167/1‑140 (MQ=255)                                   cTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGt                                                                                      >  2:765282/1‑140 (MQ=255)                                         cTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGa                                                                                <  1:41055/140‑1 (MQ=255)                                                  ccGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCa                                                                       <  2:836909/140‑1 (MQ=255)                                                                   gtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgc                                                      >  1:531302/1‑140 (MQ=255)                                                                   gtTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgc                                                      >  2:471354/1‑140 (MQ=255)                                                                      gCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAgccgcgcc                                                   >  1:8039/1‑140 (MQ=255)                                                                             ccGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACACTACCCAGCCGTGACGCCAGCCGCGCCGCTgcgc                                            <  1:818559/140‑1 (MQ=255)                                                                                cgcCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGa                                         >  1:544822/1‑140 (MQ=255)                                                                                      gTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGtt                                   >  1:125788/1‑140 (MQ=255)                                                                                         ccTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGttttt                                <  2:8039/140‑1 (MQ=255)                                                                                            gcgcCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGc                             <  1:359167/140‑1 (MQ=255)                                                                                              gcCAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTg                           >  1:907433/1‑140 (MQ=255)                                                                                                cAGACGTAAGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGcc                         <  1:13176/140‑1 (MQ=255)                                                                                                       aaGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTa                  <  1:765282/140‑1 (MQ=255)                                                                                                        aGGCGCTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTAt                 <  2:531302/140‑1 (MQ=255)                                                                                                           cgcTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGc              <  2:907433/140‑1 (MQ=255)                                                                                                             cTGAGAATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCg            <  2:125788/140‑1 (MQ=255)                                                                                                                agaATTGCCCGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATg         <  2:278571/140‑1 (MQ=255)                                                                                                                       cccGACG‑TTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATaaaa  >  2:13439/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                      ATCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGACCAGTTTTTCGCTGCCGGTGTTATGGCCGATGCATAAAA  >  NC_000913/723396‑723654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑