Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,331	(A)7→6	coding (87/906 nt)	rssA →	putative patatin‑like phospholipase RssA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,325	0	A	.	100.0%	218.9 / NA	52	coding (81/906 nt)	rssA	putative patatin‑like phospholipase RssA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (22/30);  total (22/30)

ACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACG  >  NC_000913/1289190‑1289459                                                                                                                                        |                                                                                                                                       aCCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaa                                                                                                                                    >  1:775278/1‑140 (MQ=255)    gAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaaagt                                                                                                                                 >  2:743904/1‑138 (MQ=255)    gAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaaagt                                                                                                                                 <  2:170066/140‑3 (MQ=255)    gAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGAAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaaagt                                                                                                                                 <  2:614989/140‑3 (MQ=255)      gCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaaagtgg                                                                                                                               >  1:368876/1‑136 (MQ=255)      gCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTaaaaaagtgg                                                                                                                               >  1:663198/1‑136 (MQ=255)           aGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTa                                                                                                                                        >  1:322433/1‑126 (MQ=255)           aGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTa                                                                                                                                        <  2:322433/126‑1 (MQ=255)           aGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTAtt                                                                                                                         >  2:401210/1‑140 (MQ=255)                  tgGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTg                                                                                                                  >  2:764380/1‑140 (MQ=255)                    gCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGat                                                                                                                >  1:748228/1‑140 (MQ=255)                    gCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGat                                                                                                                >  2:569528/1‑140 (MQ=255)                     cTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGata                                                                                                               <  1:457484/140‑1 (MQ=255)                          aTTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGtt                                                                                                          <  1:203592/140‑1 (MQ=255)                             gCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCa                                                                                                       <  2:663198/140‑1 (MQ=255)                             gCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCa                                                                                                       <  2:775278/140‑1 (MQ=255)                             gCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCa                                                                                                       <  2:922087/140‑1 (MQ=255)                              cATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAg                                                                                                      >  2:244529/1‑140 (MQ=255)                               aTTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAgg                                                                                                     >  1:231482/1‑140 (MQ=255)                                        gAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAAt                                                                                            <  2:346162/140‑1 (MQ=255)                                           tCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTgg                                                                                         >  1:610855/1‑140 (MQ=255)                                              tGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTc                                                                                      >  1:790301/1‑140 (MQ=255)                                                         tGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           <  1:743904/140‑1 (MQ=255)                                                         tGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           <  2:695028/140‑1 (MQ=255)                                                         tGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           <  1:818466/140‑1 (MQ=255)                                                         tGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           <  2:790301/140‑1 (MQ=255)                                                         tGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           >  2:818466/1‑140 (MQ=255)                                                           agaAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCt                                                                         <  2:610855/140‑1 (MQ=255)                                                             aaaGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGTTGGTGGGCGCTGc                                                                       <  1:458400/140‑1 (MQ=255)                                                             aaaGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGc                                                                       >  2:908220/1‑140 (MQ=255)                                                              aaGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           <  1:797538/135‑1 (MQ=255)                                                              aaGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           >  2:797538/1‑135 (MQ=255)                                                              aaGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGcc                                                                      >  2:209848/1‑140 (MQ=255)                                                                 aTAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTat                                                                   >  1:921935/1‑140 (MQ=255)                                                                    aaaaTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTatgca                                                                <  1:764380/140‑1 (MQ=255)                                                                       aTAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTatgcatgc                                                             <  2:307106/140‑1 (MQ=255)                                                                        tAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcg                                                            <  1:747363/140‑1 (MQ=255)                                                                           ggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           <  2:813062/122‑1 (MQ=255)                                                                           ggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGgcg                                                                           >  1:813062/1‑122 (MQ=255)                                                                           ggCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGcgatc                                                         >  1:899826/1‑140 (MQ=255)                                                                                gcgcTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTa                                                    >  1:395416/1‑140 (MQ=255)                                                                                  gcTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATc                                                  <  1:209848/140‑1 (MQ=255)                                                                                    tGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTg                                                <  2:899826/140‑1 (MQ=255)                                                                                      ggATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTgcg                                              <  1:777044/140‑1 (MQ=255)                                                                                       gATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTgcgc                                             <  1:569528/140‑1 (MQ=255)                                                                                                  gcgAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTgg                                  >  2:901432/1‑140 (MQ=255)                                                                                                       agGTTGGTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGAc                             <  2:748228/140‑1 (MQ=255)                                                                                                             gTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCttt                       >  1:600719/1‑140 (MQ=255)                                                                                                             gTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCttt                       <  1:671413/140‑1 (MQ=255)                                                                                                             gTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCttt                       <  1:908220/140‑1 (MQ=255)                                                                                                             gTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGCTCGCTGGTGGGCGCTGCCTACGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCttt                       <  2:117828/140‑1 (MQ=255)                                                                                                             gTCGCATATTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGCTCGCTGGTGGGCGCTGCCTACGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCttt                       >  1:117828/1‑140 (MQ=255)                                                                                                                  atatTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTc                      <  1:549852/136‑1 (MQ=255)                                                                                                                  atatTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTc                      >  2:549852/1‑136 (MQ=255)                                                                                                                  atatTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTc                      >  2:841751/1‑136 (MQ=255)                                                                                                                  atatTGGCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTc                      <  1:841751/136‑1 (MQ=255)                                                                                                                        gCGTTATTAATGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTggg            <  2:921935/140‑1 (MQ=255)                                                                                                                                  tGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTggg            >  2:694457/1‑130 (MQ=255)                                                                                                                                  tGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTggg            <  1:694457/130‑1 (MQ=255)                                                                                                                                  tGCGCT‑AAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACg  <  2:368876/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAATAGGGCTGGCGCTGGGATCTGGCGCGGCGAGAGGTTGGTCGCATATTGGCGTTATTAATGCGCTAAAAAAAGTGGGTATTGAAATTGATATCGTTGCAGGATGTTCAATTGGTTCGCTGGTGGGCGCTGCCTATGCATGCGATCGATTATCTGCGCTGGAAGATTGGGTGACCTCTTTCAGTTATTGGGATGTTTTACG  >  NC_000913/1289190‑1289459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑