Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,286,291	(A)5→4	intergenic (+4/+99)	proL → / ← yejO	tRNA‑Pro/adhesin‑like autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,286,287	0	A	.	100.0%	127.8 / NA	31	noncoding (77/77 nt)	proL	tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (14/17);  total (14/17)

GTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCAC  >  NC_000913/2286151‑2286407                                                                                                                                         |                                                                                                                         gTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCaaaa                                                                                                                       >  2:388181/1‑140 (MQ=255)     gCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCaaaatccc                                                                                                                   <  1:184653/140‑5 (MQ=255)     gCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCaaaatccc                                                                                                                   <  2:282046/140‑5 (MQ=255)      cGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCa                                                                                                                 >  1:443466/1‑140 (MQ=255)            atCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGaaaa                                                                                                           >  1:29336/1‑140 (MQ=255)                tACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAaacc                                                                                                       <  2:290728/140‑1 (MQ=255)                tACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAaacc                                                                                                       <  1:355132/140‑1 (MQ=255)                     gCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAAccc                                                                                                  >  1:644042/1‑140 (MQ=255)                     gCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAAccc                                                                                                  >  2:509567/1‑140 (MQ=255)                      cAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCt                                                                                                 <  2:29336/140‑1 (MQ=255)                      cAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCt                                                                                                 <  1:388181/140‑1 (MQ=255)                           ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACgg                                                                                            <  1:509567/140‑1 (MQ=255)                            gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACggt                                                                                           <  2:443466/140‑1 (MQ=255)                            gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACggt                                                                                           <  2:533683/140‑1 (MQ=255)                               tgGAAAACGGTAGTACTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACggttgg                                                                                        <  1:166174/140‑1 (MQ=255)                                                agcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaa                                                                       >  1:346766/1‑140 (MQ=255)                                                 gcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                      >  2:591420/1‑140 (MQ=255)                                                 gcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                      <  2:69415/140‑1 (MQ=255)                                                      cACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                 >  2:341049/1‑140 (MQ=255)                                                       aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTc                                                                >  2:505605/1‑140 (MQ=255)                                                        cGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaat                                                                  <  2:131066/137‑1 (MQ=255)                                                        cGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaat                                                                  >  1:131066/1‑137 (MQ=255)                                                           gTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGCTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATa                                                            >  1:636449/1‑140 (MQ=255)                                                                  cGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGAc                                                     <  2:644042/140‑1 (MQ=255)                                                                                      cACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt                                 <  1:591420/140‑1 (MQ=255)                                                                                          gTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAAACCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAt                             <  2:636449/140‑1 (MQ=255)                                                                                                gggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                       <  1:716893/140‑1 (MQ=255)                                                                                                   tgtCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTa                    >  1:156949/1‑140 (MQ=255)                                                                                                     tCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                  >  2:16900/1‑140 (MQ=255)                                                                                                     tCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                  >  1:811091/1‑140 (MQ=255)                                                                                                        ggggTCGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa               <  1:16900/140‑1 (MQ=255)                                                                                                             cGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTc          <  2:346766/140‑1 (MQ=255)                                                                                                             cGGAGGTTCAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTCACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTc          <  2:798370/140‑1 (MQ=255)                                                                                                                     cAAATCCTCTCGTGCCGACC‑AAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACcac  >  1:286072/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                         GTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCAC  >  NC_000913/2286151‑2286407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑