Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,575,000	(T)6→5	coding (4/702 nt) coding (458/480 nt)	eutQ ← eutP ←	putative ethanolamine utilization protein EutQ putative ethanolamine utilization protein EutP with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,995	0	T	.	100.0%	223.4 / NA	53	coding (9/702 nt) coding (463/480 nt)	eutQ eutP	putative ethanolamine utilization protein EutQ putative ethanolamine utilization protein EutP with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (28/25);  total (28/25)

ACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTT  >  NC_000913/2574860‑2575128                                                                                                                                        |                                                                                                                                      acaTTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGttttt                                                                                                                                   <  2:754351/140‑1 (MQ=255)    ttCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttttcac                                                                                                                                <  2:907822/140‑4 (MQ=255)    ttCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttttcac                                                                                                                                <  1:738577/140‑4 (MQ=255)     tCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttttcacc                                                                                                                               >  2:202872/1‑136 (MQ=255)       ggTGATGGTAAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTg                                                                                                                            <  2:71185/140‑1 (MQ=255)         tGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCt                                                                                                                             >  2:586400/1‑137 (MQ=255)         tGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCt                                                                                                                             <  1:586400/137‑1 (MQ=255)            tGGTAAACCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttttca                                                                                                                                 <  2:769172/131‑3 (MQ=255)            tGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGtttttca                                                                                                                                 >  1:769172/1‑129 (MQ=255)            tGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTtcc                                                                                                                       >  2:122458/1‑140 (MQ=255)                 aaaCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTg                                                                                                                            >  1:130846/1‑130 (MQ=255)                 aaaCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTg                                                                                                                            <  2:130846/130‑1 (MQ=255)                  aaCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGt                                                                                                                 <  2:129160/140‑1 (MQ=255)                  aaCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGt                                                                                                                 >  2:494820/1‑140 (MQ=255)                  aaCCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGt                                                                                                                 <  1:451540/140‑1 (MQ=255)                              ccGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGAt                                                                                                     <  1:180285/140‑1 (MQ=255)                              ccGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGAt                                                                                                     <  1:18496/140‑1 (MQ=255)                               cGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATg                                                                                                    >  1:622859/1‑140 (MQ=255)                                  cACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCa                                                                                                 <  1:212156/140‑1 (MQ=255)                                   aCTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAg                                                                                                >  2:749197/1‑140 (MQ=255)                                    cTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGa                                                                                               <  2:622859/140‑1 (MQ=255)                                    cTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGa                                                                                               <  1:749197/140‑1 (MQ=255)                                     ttCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGAt                                                                                              >  1:790137/1‑140 (MQ=255)                                        aCGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATCGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATg                                                                                                    >  2:824941/1‑131 (MQ=255)                                        aCGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATCGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATg                                                                                                    <  1:824941/131‑1 (MQ=255)                                            gCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATccac                                                                                       >  1:575231/1‑140 (MQ=255)                                            gCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATccac                                                                                       >  1:886361/1‑140 (MQ=255)                                               tCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAg                                                                                    >  2:482939/1‑140 (MQ=255)                                               tCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAg                                                                                    >  2:370327/1‑140 (MQ=255)                                                              cTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTg                                                                     >  1:128518/1‑140 (MQ=255)                                                              cTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTg                                                                     >  1:272405/1‑140 (MQ=255)                                                                ggCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACTAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCGTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCg                                                                   >  2:605023/1‑140 (MQ=255)                                                                ggCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCg                                                                   >  1:368759/1‑140 (MQ=255)                                                                ggCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCg                                                                   <  1:428051/140‑1 (MQ=255)                                                                   gcgcAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGt                                                                <  1:517129/140‑1 (MQ=255)                                                                     gcAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCa                                                                            >  2:8387/1‑126 (MQ=255)                                                                     gcAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCa                                                                            <  1:8387/126‑1 (MQ=255)                                                                     gcAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCg                                                              <  2:128518/140‑1 (MQ=255)                                                                       aGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTg                                                            <  1:370327/140‑1 (MQ=255)                                                                              cGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGttt                                                     <  1:221507/140‑1 (MQ=255)                                                                                    ttGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCg                                               <  2:575231/140‑1 (MQ=255)                                                                                     tGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTgg                                                           <  1:124125/127‑1 (MQ=255)                                                                                     tGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTgg                                                           >  2:124125/1‑127 (MQ=255)                                                                                         tGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACAt                                          >  2:336304/1‑140 (MQ=255)                                                                                           ttCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCg                                        >  2:705715/1‑140 (MQ=255)                                                                                             cgccgcGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGc                                      <  2:795456/140‑1 (MQ=255)                                                                                               ccgcGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTTGCTGTTTAGCTCGaa                                             <  2:89674/131‑1 (MQ=255)                                                                                               ccgcGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTTGCTGTTTAGCTCGaa                                             >  1:89674/1‑131 (MQ=255)                                                                                                cgcgcgcgTGTGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGctct                                   >  2:689898/1‑140 (MQ=255)                                                                                                       gtgtGCTTCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAAc                            <  2:290396/140‑1 (MQ=255)                                                                                                              tCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGtctc                     >  2:88288/1‑140 (MQ=255)                                                                                                              tCACGAATATCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGtctc                     <  1:460846/140‑1 (MQ=255)                                                                                                                    atatCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCg                                <  2:580793/123‑1 (MQ=255)                                                                                                                    atatCATTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCg                                >  1:580793/1‑123 (MQ=255)                                                                                                                         aTTCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACt          >  2:457668/1‑140 (MQ=255)                                                                                                                          ttCGCTGTGATGAG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCCCGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACtt         <  2:361566/140‑1 (MQ=255)                                                                                                                               tgtgATGAG‑TTTTTCACCTGCGTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTcgcg    >  2:649447/1‑140 (MQ=255)                                                                                                                                 tgatgaG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGtt  >  2:392356/1‑140 (MQ=255)                                                                                                                                   atgaG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAAt              <  1:505192/126‑1 (MQ=255)                                                                                                                                   atgaG‑TTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAAt              >  2:505192/1‑126 (MQ=255)                                                                                                                                        |                                                                                                                                      ACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAATCCACCAGTTGCTGCACGCTTTGCGGGTCGTGGCTGTTTAGCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTT  >  NC_000913/2574860‑2575128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑