Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,785,204	(A)8→7	intergenic (‑193/+558)	ypjC ← / ← ileY	DUF5507 domain‑containing protein YpjC/tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,785,197	0	A	.	100.0%	180.9 / NA	43	intergenic (‑186/+565)	ypjC/ileY	DUF5507 domain‑containing protein YpjC/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (27/16);  total (27/16)

GTTTGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATAAAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTT  >  NC_000913/2785061‑2785330                                                                                                                                         |                                                                                                                                      gTTTGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATaaaa                                                                                                                                    <  1:57579/140‑1 (MQ=255)  tttGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATaaaaa                                                                                                                                   <  2:46968/140‑1 (MQ=255)  tttGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATaaaaa                                                                                                                                   <  1:208140/140‑1 (MQ=255)    tGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATaaaaaaa                                                                                                                                 >  2:655196/1‑140 (MQ=255)        aCTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATaaaaaaaccgt                                                                                                                             <  1:529230/140‑5 (MQ=255)            taAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTtata                                                                                                                        >  1:802012/1‑140 (MQ=255)                            tGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAAt                                                                                                        <  1:152497/140‑1 (MQ=255)                                   aTTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGttttt                                                                                                 >  2:734333/1‑140 (MQ=255)                                    ttACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTc                                                                                                >  1:675937/1‑140 (MQ=255)                                    ttACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTACGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTc                                                                                                >  1:362919/1‑140 (MQ=255)                                     tACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCa                                                                                               >  1:907472/1‑140 (MQ=255)                                      aCTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCaa                                                                                              <  2:907472/140‑1 (MQ=255)                                           cATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGtt                                                                                         <  1:610947/140‑1 (MQ=255)                                            aTTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTCAAAATCGTTTTTCAAGTGTTa                                                                                        >  2:778864/1‑140 (MQ=255)                                            aTTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTACGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTa                                                                                        <  2:362919/140‑1 (MQ=255)                                             ttAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGt                                                                                                     <  1:623767/126‑1 (MQ=255)                                             ttAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGt                                                                                                     >  2:623767/1‑126 (MQ=255)                                              tAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGa                                                                                      >  1:181848/1‑140 (MQ=255)                                                    cTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTta                                                                                >  1:171988/1‑140 (MQ=255)                                                     ttCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGttttt                                                                                                 >  2:12698/1‑122 (MQ=255)                                                     ttCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGttttt                                                                                                 <  1:12698/122‑1 (MQ=255)                                                       cACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTatat                                                                             <  1:532026/140‑1 (MQ=255)                                                            gCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCaa                                                                        >  2:193218/1‑140 (MQ=255)                                                             cTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAAt                                                                       >  2:323654/1‑140 (MQ=255)                                                                            ttaattaaATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGa                                                        >  1:399193/1‑140 (MQ=255)                                                                             taattaaATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAg                                                       >  1:98588/1‑140 (MQ=255)                                                                             taattaaATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAg                                                       <  2:675937/140‑1 (MQ=255)                                                                                     ttaGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCa                                               <  2:399193/140‑1 (MQ=255)                                                                                     ttaGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCa                                               <  2:547425/140‑1 (MQ=255)                                                                                     ttaGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCa                                               <  2:504000/140‑1 (MQ=255)                                                                                      taGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCAc                                              <  2:98588/140‑1 (MQ=255)                                                                                         cacaGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCt                                           >  2:449222/1‑140 (MQ=255)                                                                                              gAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCa                                               >  2:474094/1‑131 (MQ=255)                                                                                              gAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCa                                               <  1:474094/131‑1 (MQ=255)                                                                                              gAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGt                                      >  2:511344/1‑140 (MQ=255)                                                                                              gAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGt                                      >  2:616570/1‑140 (MQ=255)                                                                                               aaTGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTa                                     >  1:456376/1‑140 (MQ=255)                                                                                                      aaTTTAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTca                              >  1:28439/1‑140 (MQ=255)                                                                                                        tttAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTcaca                            >  1:449221/1‑140 (MQ=255)                                                                                                        tttAATAAACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTcaca                            >  2:897822/1‑140 (MQ=255)                                                                                                           aataaACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACaaaa                         >  1:152317/1‑140 (MQ=255)                                                                                                           aataaACAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACaaaa                         >  1:519846/1‑140 (MQ=255)                                                                                                                aCAAAAGGTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCTCGCTCCTGTAACGCTCACAAAACTCAt                    >  2:166022/1‑140 (MQ=255)                                                                                                                       gTTATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATctgcctg             <  1:914359/140‑1 (MQ=255)                                                                                                                             tCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGcggcgg       <  1:616570/140‑1 (MQ=255)                                                                                                                              cGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCggg      >  1:809524/1‑140 (MQ=255)                                                                                                                                  gTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGtt  >  2:607393/1‑140 (MQ=255)                                                                                                                                  gTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGtt  <  1:784302/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GTTTGTCACTATAAAAAAACGCTCATTTGAGACAATTACTGACATTAACTGCTTCACTTGCTACGCATGGAACTTTTAATTAAATTAGCACAGGAATGTTAAATTTAATAAACAAAAGGTTATTTCGCTGTATGATAAAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTT  >  NC_000913/2785061‑2785330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑