Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,580	(G)7→8	noncoding (73/88 nt)	serX ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,573	1	.	G	91.7%	34.7 / ‑2.9	12	noncoding (80/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (0/11);  total (0/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCA  >  NC_000913/1097455‑1097701                                                                                                                        |                                                                                                                                 ccAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCg                                                                                                              <  1:135736/140‑1 (MQ=255)         cTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTg                                                                                                      <  2:90143/140‑1 (MQ=255)                         aaCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTc                                                                                      <  2:303221/140‑1 (MQ=255)                                 tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTg                                                                              <  1:370164/140‑1 (MQ=255)                                 tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTg                                                                              <  1:676130/140‑1 (MQ=255)                                         tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCa                                                                      <  1:222456/140‑1 (MQ=255)                                          ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGCGTTGCAGCCCCCCCGCGTTGCCGTATACACACTTTCCACGGGTGCTCCTTccg                                                                     >  2:796210/1‑138 (MQ=14)                                           tcACCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGcc                                                                   <  1:24888/140‑1 (MQ=255)                                              tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCAc                                                                 <  1:32515/140‑1 (MQ=255)                                                                        aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTc                                       <  1:302189/140‑1 (MQ=255)                                                                                   tACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGGTCGGAACCCCCCTACGTTTCCGTATACACACCTTCCAGGCGGGCTCCCTCCGCCCCCCCGACACCCCACCCAAATGTCGTTTCTCTCTTTCtt                            >  2:302189/1‑139 (MQ=2)                                                                                            aCGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgcg                   <  1:488064/140‑1 (MQ=255)                                                                                                          aaaTATGGCGGTGAGGGGGGGGGGTCGAAACCCCCCCACGTTGCCGTATAAACACCTTCCCGGCGGGGTCCTTCCGCCACCCGGGCACCCCCCCCAAATTTCGTTCCTGTCTTTCTGGGACGGGGGCCAATTTTGGGgaa     >  2:628681/1‑140 (MQ=9)                                                                                                            atatGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATc   <  1:891577/140‑1 (MQ=255)                                                                                                             tatGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCa  <  1:849699/140‑1 (MQ=255)                                                                                                                        |                                                                                                                                 CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCA  >  NC_000913/1097455‑1097701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑