Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,026	(C)6→7	coding (627/1179 nt)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,020	1	.	C	100.0%	157.3 / NA	44	G211G (GGC→GGG)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (21/23);  total (21/23)

CGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTA  >  NC_000913/2637884‑2638152                                                                                                                                          |                                                                                                                                     cGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑ccc                                                                                                                                   >  1:276230/1‑140 (MQ=255)        aacaacGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑cccccccacg                                                                                                                            >  2:810910/1‑136 (MQ=255)             cgacgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGc                                                                                                                        >  2:126457/1‑140 (MQ=255)                cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGc                                                                                                                     >  1:652997/1‑140 (MQ=255)                cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGc                                                                                                                     >  1:250097/1‑140 (MQ=255)                  ggAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCAc                                                                                                                   >  2:476217/1‑140 (MQ=255)                    aGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCa                                                                                                                 <  1:667270/140‑1 (MQ=255)                               cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGt                                                                                                      <  2:23317/140‑1 (MQ=255)                               cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGt                                                                                                      <  1:476217/140‑1 (MQ=255)                                    cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCa                                                                                                                 >  1:378304/1‑124 (MQ=255)                                    cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCa                                                                                                                 <  2:378304/124‑1 (MQ=255)                                    cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                                 >  1:818102/1‑140 (MQ=255)                                    cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                                 <  1:683598/140‑1 (MQ=255)                                         aCGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGAc                                                                                            >  1:535585/1‑140 (MQ=255)                                             tCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                                        <  2:818102/140‑1 (MQ=255)                                                aTTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCAc                                                                                     <  1:810910/140‑1 (MQ=255)                                                      gTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAag                                                                               <  2:250097/140‑1 (MQ=255)                                                       tAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAaga                                                                              >  1:104138/1‑140 (MQ=255)                                                            ccGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCg                                                                         <  2:652997/140‑1 (MQ=255)                                                               gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                                      >  1:532218/1‑140 (MQ=255)                                                               gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                                      >  1:324077/1‑140 (MQ=255)                                                                    cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                                 <  2:435927/140‑1 (MQ=255)                                                                    cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                                 <  1:288278/140‑1 (MQ=255)                                                                    cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                                 <  2:276230/140‑1 (MQ=255)                                                                            tACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGGCCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGtt                                                         >  2:448370/1‑140 (MQ=255)                                                                              cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCg                                                                         >  1:587107/1‑122 (MQ=255)                                                                              cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCg                                                                         <  2:587107/122‑1 (MQ=255)                                                                              cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTaa                                                       >  1:81897/1‑140 (MQ=255)                                                                                 tgctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                                    >  2:203231/1‑140 (MQ=255)                                                                                 tgctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                                    >  2:337030/1‑140 (MQ=255)                                                                                  gctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGt                                                   >  2:748542/1‑140 (MQ=255)                                                                                             atcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAg                                        >  1:12649/1‑140 (MQ=255)                                                                                              tcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgc                                       <  1:718074/140‑1 (MQ=255)                                                                                                   cccTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGt                                  <  2:104138/140‑1 (MQ=255)                                                                                                   cccTGTTCCATCAGCACTACGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCACATCGAGGTTAACTGTCCATTTGACAGCGCCGt                                  <  1:472834/140‑1 (MQ=255)                                                                                                     cTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCa                                <  2:532218/140‑1 (MQ=255)                                                                                                               aGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCa                      <  1:522705/140‑1 (MQ=255)                                                                                                               aGCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCa                      <  2:870241/140‑1 (MQ=255)                                                                                                                gCACTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAg                     <  1:748542/140‑1 (MQ=255)                                                                                                                 cACTGCGCTGACGCGGCCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAgc                    <  1:448370/140‑1 (MQ=255)                                                                                                                   cTGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAgcgc                  <  2:830766/140‑1 (MQ=255)                                                                                                                    tGCGCTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCt                 >  1:242829/1‑140 (MQ=255)                                                                                                                      cgcTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTg               >  2:670069/1‑140 (MQ=255)                                                                                                                      cgcTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTg               >  1:35647/1‑140 (MQ=255)                                                                                                                       gcTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCt                             >  2:234549/1‑125 (MQ=255)                                                                                                                       gcTGACGCGACCATTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCt                             <  1:234549/125‑1 (MQ=255)                                                                                                                                   aTTATCGCCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTCGTAACTGACCGTTa  <  2:237519/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTA  >  NC_000913/2637884‑2638152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑