Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,776	T→C	F796L (TTT→CTT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,776	0	T	C	100.0%	165.3 / NA	46	F796L (TTT→CTT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

CGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTA  >  NC_000913/24642‑24910                                                                                                                                       |                                                                                                                                       cgcTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGc                                                                                                                                   <  1:565272/140‑1 (MQ=255)      tatCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGc                                                                                                                              >  2:724206/1‑140 (MQ=255)           caTCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGa                                                                                                                           >  2:640154/1‑138 (MQ=255)           caTCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGa                                                                                                                           <  1:640154/138‑1 (MQ=255)           caTCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACa                                                                                                                         >  1:567588/1‑140 (MQ=255)            aTCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAg                                                                                                                        >  2:746231/1‑140 (MQ=255)              cGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTg                                                                                                                      >  2:63392/1‑140 (MQ=255)              cGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTg                                                                                                                      >  1:325571/1‑140 (MQ=255)                            tGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGAt                                                                                                        <  2:292883/140‑1 (MQ=255)                                          ccAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGa                                                                                          <  1:63392/140‑1 (MQ=255)                                            aaTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGc                                                                                        <  2:263855/140‑1 (MQ=255)                                             aTCCTCTCCTTCACCGCTGATGGAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGAt                                                                                                        >  2:74383/1‑123 (MQ=255)                                             aTCCTCTCCTTCACCGCTGATGGAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGAt                                                                                                        <  1:74383/123‑1 (MQ=255)                                             aTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGAt                                                                                                        >  1:605218/1‑123 (MQ=255)                                             aTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGAt                                                                                                        <  2:605218/123‑1 (MQ=255)                                             aTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGc                                                                                        >  1:440429/1‑139 (MQ=255)                                             aTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGc                                                                                        <  2:440429/139‑1 (MQ=255)                                             aTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCt                                                                                       >  2:109746/1‑140 (MQ=255)                                              tCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTg                                                                                      <  2:486393/140‑1 (MQ=255)                                                    ccTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGact                                                                                >  1:401136/1‑138 (MQ=255)                                                     cTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAg                                                                                         <  2:647895/130‑1 (MQ=255)                                                     cTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAg                                                                                         >  1:647895/1‑130 (MQ=255)                                                        cACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGc                                                                            >  2:118632/1‑140 (MQ=255)                                                               gatgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGa                                                                     <  1:444206/140‑1 (MQ=255)                                                                atgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGaa                                                                    <  1:109746/140‑1 (MQ=255)                                                                   aaGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTg                                                                 <  1:118632/140‑1 (MQ=255)                                                                          gggCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAg                                                          >  1:341422/1‑140 (MQ=255)                                                                             cTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCa                                                       <  2:325571/140‑1 (MQ=255)                                                                              tACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCAt                                                      <  1:211385/140‑1 (MQ=255)                                                                                ccTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAg                                                                   <  2:265574/125‑1 (MQ=255)                                                                                ccTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAg                                                                   >  1:265574/1‑125 (MQ=255)                                                                                ccTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACa                                                             >  1:650905/1‑131 (MQ=255)                                                                                ccTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACa                                                             <  2:650905/131‑1 (MQ=255)                                                                                       ggCGAACGTGAAAAATACGTATTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                                             >  2:680844/1‑140 (MQ=255)                                                                                                 aaaaaTACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGAc                                   <  2:493623/140‑1 (MQ=255)                                                                                                      tACGTCTTCACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGaa                              <  1:38222/140‑1 (MQ=255)                                                                                                              cACCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAgtgggtg                      >  1:817233/1‑140 (MQ=255)                                                                                                               aCCGGTGAGTGGTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAgtgggtgg                     <  1:746231/140‑1 (MQ=255)                                                                                                                         ggTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGaa           >  1:768897/1‑140 (MQ=255)                                                                                                                          gTACGAAGGCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAg          <  1:680844/140‑1 (MQ=255)                                                                                                                                  gCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCg                 >  2:376707/1‑125 (MQ=255)                                                                                                                                  gCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCg                 <  1:376707/125‑1 (MQ=255)                                                                                                                                  gCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCgg       >  1:757654/1‑135 (MQ=255)                                                                                                                                  gCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCgg       <  2:757654/135‑1 (MQ=255)                                                                                                                                  gCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTa  >  1:539687/1‑140 (MQ=255)                                                                                                                                  gCCTGCTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTa  <  2:768897/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTA  >  NC_000913/24642‑24910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑