Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	296,675	A→G	V141A (GTC→GCC)	intF ←	CP4‑6 prophage; putative phage integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	296,675	0	A	G	100.0%	250.6 / NA	69	V141A (GTC→GCC)	intF	CP4‑6 prophage; putative phage integrase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/34);  total (35/34)

CCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTCTTGCCTCGGCTCTCGCAT  >  NC_000913/296540‑296810                                                                                                                                        |                                                                                                                                        ccTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGga                                                                                                                                     <  1:604850/140‑1 (MQ=255)       ccTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGt                                                                                                                               <  2:93475/140‑1 (MQ=255)          ctTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCAc                                                                                                                            >  2:440062/1‑140 (MQ=255)          ctTTTTTCACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCAc                                                                                                                            >  2:832373/1‑140 (MQ=255)           tttttttACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACt                                                                                                                           >  1:29974/1‑140 (MQ=255)           tttttttACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACt                                                                                                                           <  1:440062/140‑1 (MQ=255)           ttttttcACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACt                                                                                                                           <  1:832373/140‑1 (MQ=255)                         ccTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGa                                                                                                             >  1:386137/1‑140 (MQ=255)                           tCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGAtt                                                                                                           <  2:54066/140‑1 (MQ=255)                             cACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTct                                                                                                         <  2:316074/140‑1 (MQ=255)                                     gACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAg                                                                                                 >  1:115075/1‑140 (MQ=255)                                      aCAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAgg                                                                                                <  2:29974/140‑1 (MQ=255)                                               tgtgATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGAttctgct                                                                                       >  2:658790/1‑140 (MQ=255)                                                    tCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCa                                                                                                  >  2:354242/1‑124 (MQ=255)                                                    tCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCa                                                                                                  <  1:354242/124‑1 (MQ=255)                                                    tCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGAttctgcttctgc                                                                                  >  2:52269/1‑140 (MQ=255)                                                    tCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGAttctgcttctgc                                                                                  >  1:556497/1‑140 (MQ=255)                                                    tCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGAtt                                                                                            >  2:733895/1‑130 (MQ=255)                                                    tCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGAtt                                                                                            <  1:733895/130‑1 (MQ=255)                                                     cGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCg                                                                                 >  2:775965/1‑140 (MQ=255)                                                      ggCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGa                                                                                <  1:519498/140‑1 (MQ=255)                                                           tGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGAttttt                                                                           >  2:376668/1‑140 (MQ=255)                                                           tGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGAttttt                                                                           >  1:877459/1‑140 (MQ=255)                                                             tATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTAc                                                                         >  2:221738/1‑140 (MQ=255)                                                              aTCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACa                                                                        <  1:407365/140‑1 (MQ=255)                                                                  aGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGAttttt                                                                           >  2:864084/1‑133 (MQ=255)                                                                  aGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGAttttt                                                                           <  1:864084/133‑1 (MQ=255)                                                                  aGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCt                                                                    >  1:718953/1‑140 (MQ=255)                                                                  aGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCt                                                                    >  1:737693/1‑140 (MQ=255)                                                                       aaTATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGa                                                                                <  2:885038/123‑1 (MQ=255)                                                                       aaTATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGa                                                                                >  1:885038/1‑123 (MQ=255)                                                                            gggCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCg                                                          >  1:234537/1‑140 (MQ=255)                                                                            gggCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCg                                                          >  2:319763/1‑140 (MQ=255)                                                                             ggCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                                         >  2:578382/1‑140 (MQ=255)                                                                             ggCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                                         <  2:683546/140‑1 (MQ=255)                                                                             ggCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                                         >  1:633890/1‑140 (MQ=255)                                                                               cGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTgg                                                       <  2:737693/140‑1 (MQ=255)                                                                                gTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGa                                                      >  2:363034/1‑140 (MQ=255)                                                                                 tttAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCg                                                          >  2:763778/1‑135 (MQ=255)                                                                                 tttAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCg                                                          <  1:763778/135‑1 (MQ=255)                                                                                 tttAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                                         >  2:183065/1‑136 (MQ=255)                                                                                 tttAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                                         <  1:183065/136‑1 (MQ=255)                                                                                 tttAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGAt                                                     <  2:99552/140‑1 (MQ=255)                                                                                  ttAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                                                    <  2:234537/140‑1 (MQ=255)                                                                                  ttAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                                                    <  2:165211/140‑1 (MQ=255)                                                                                   tAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCt                                                   >  2:82387/1‑140 (MQ=255)                                                                                       ttttGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATcc                                               <  2:718953/140‑1 (MQ=255)                                                                                             aCTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTAt                                         <  2:115075/140‑1 (MQ=255)                                                                                                gATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGa                                      >  1:412509/1‑140 (MQ=255)                                                                                                 aTACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGAt                                     <  2:877459/140‑1 (MQ=255)                                                                                                 aTACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGAt                                     >  2:453347/1‑140 (MQ=255)                                                                                                   aCCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCa                                   <  1:775965/140‑1 (MQ=255)                                                                                                    ccGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATcc                                               <  2:839975/127‑1 (MQ=255)                                                                                                    ccGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATcc                                               >  1:839975/1‑127 (MQ=255)                                                                                                       gTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGt                               >  1:162085/1‑140 (MQ=255)                                                                                                           tcAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGt                           >  1:485378/1‑140 (MQ=255)                                                                                                           tcAATTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGt                           >  2:454245/1‑140 (MQ=255)                                                                                                             aaTTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTaa                         <  1:52269/140‑1 (MQ=255)                                                                                                             aaTTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTaa                         <  1:454245/140‑1 (MQ=255)                                                                                                             aaTTCTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTaa                         <  1:319763/140‑1 (MQ=255)                                                                                                                 cTTGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGt                     <  1:453347/140‑1 (MQ=255)                                                                                                                  ttGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTc                    <  1:82387/140‑1 (MQ=255)                                                                                                                  ttGAAGATAGTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTc                    <  2:556497/140‑1 (MQ=255)                                                                                                                        aTAGTCTTTCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTCTTGCCt              <  2:633890/140‑1 (MQ=255)                                                                                                                           gTCTTCCCAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTCTTGCCTCgg           >  2:572543/1‑140 (MQ=255)                                                                                                                                cccAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGc                                                                      >  1:812362/1‑76 (MQ=255)                                                                                                                                cccAGGCGGCGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGc                                                                      <  2:812362/76‑1 (MQ=255)                                                                                                                                 ccAGGCGGCGGAGAAAGTCACTTTTGTCTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTCTTGcc               <  1:342001/130‑1 (MQ=255)                                                                                                                                 ccAGGCGGCGGAGAAAGTCACTTTTGTCTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTCTTGcc               >  2:342001/1‑130 (MQ=255)                                                                                                                                    ggcggcGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTCTTGCCTCGGCTCTCGcat  <  2:283911/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTGATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAGAAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAACCGTCTTGCCTCGGCTCTCGCAT  >  NC_000913/296540‑296810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑