Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,073,784	T→C	intergenic (‑93/+192)	epd ← / ← yggC	D‑erythrose‑4‑phosphate dehydrogenase/P‑loop NTPase domain‑containing protein YggC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,073,784	0	T	C	100.0%	217.1 / NA	60	intergenic (‑93/+192)	epd/yggC	D‑erythrose‑4‑phosphate dehydrogenase/P‑loop NTPase domain‑containing protein YggC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/26);  total (34/26)

CCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACTGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTG  >  NC_000913/3073651‑3073919                                                                                                                                      |                                                                                                                                        ccGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTgg                                                                                                                                   >  1:782279/1‑140 (MQ=255)            gAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGa                                                                                                                        <  2:36298/140‑1 (MQ=255)             aaGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGaa                                                                                                                       <  1:737063/140‑1 (MQ=255)             aaGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGaa                                                                                                                       >  1:283098/1‑140 (MQ=255)                ccATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACAt                                                                                                                    <  1:205467/140‑1 (MQ=255)                    ttATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTaa                                                                                                                >  1:760419/1‑140 (MQ=255)                              gcgTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGaaa                                                                                                      <  1:309028/140‑1 (MQ=255)                               cgTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGa                                                                                                            <  2:703822/133‑1 (MQ=255)                               cgTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGa                                                                                                            >  1:703822/1‑133 (MQ=255)                                 tACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATc                                                                                                              >  1:834844/1‑129 (MQ=255)                                 tACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATc                                                                                                              <  2:834844/129‑1 (MQ=255)                                  aCGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTGGAACATTTAATCGACTGAAACGCt                                                                                                  <  1:593381/140‑1 (MQ=255)                                      tCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTGATTGTCGAACATTTAATCGACTGAAACGCTTCAg                                                                                              <  1:410867/140‑1 (MQ=255)                                            aTCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGa                                                                                        <  2:310403/140‑1 (MQ=255)                                                ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGc                                                                                             >  2:754072/1‑131 (MQ=255)                                                ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGc                                                                                             <  1:754072/131‑1 (MQ=255)                                                ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                    >  2:736154/1‑140 (MQ=255)                                                ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                    >  2:601231/1‑140 (MQ=255)                                                ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                    >  2:294250/1‑140 (MQ=255)                                                ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                    >  1:79329/1‑140 (MQ=255)                                                ccTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAg                                                                                    >  2:918815/1‑140 (MQ=255)                                                    cAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGaa                                                                                >  1:388985/1‑140 (MQ=255)                                                      aGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAAc                                                                              >  2:142069/1‑140 (MQ=255)                                                       ggTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACg                                                                             <  2:550837/140‑1 (MQ=255)                                                        gTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGt                                                                            >  1:250603/1‑140 (MQ=255)                                                        gTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGt                                                                            <  1:918815/140‑1 (MQ=255)                                                           ttCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGa                                                                         >  2:556757/1‑140 (MQ=255)                                                             cccTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAAt                                                                       <  2:388985/140‑1 (MQ=255)                                                                  aGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAg                                                                  >  1:714754/1‑140 (MQ=255)                                                                  aGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAg                                                                  >  2:5299/1‑140 (MQ=255)                                                                  aGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAg                                                                  <  1:601231/140‑1 (MQ=255)                                                                   gCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGaa                                                                        >  1:595765/1‑133 (MQ=255)                                                                   gCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGaa                                                                        <  2:595765/133‑1 (MQ=255)                                                                           gCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTg                                                         <  2:782279/140‑1 (MQ=255)                                                                                 cagaGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGc                                                   >  1:42326/1‑140 (MQ=255)                                                                                 cagaGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGc                                                   <  1:736154/140‑1 (MQ=255)                                                                                 cagaGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGc                                                   >  1:300384/1‑140 (MQ=255)                                                                                      tAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCgaag                                              >  2:31025/1‑140 (MQ=255)                                                                                         tCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGaagaag                                           >  1:581200/1‑140 (MQ=255)                                                                                            aGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACa                                        >  2:780638/1‑140 (MQ=255)                                                                                              cAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCgaaga                                             >  1:687445/1‑133 (MQ=255)                                                                                              cAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCgaaga                                             <  2:687445/133‑1 (MQ=255)                                                                                              cAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCg                                                 >  1:915236/1‑129 (MQ=255)                                                                                              cAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCg                                                 <  2:915236/129‑1 (MQ=255)                                                                                              cAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAtt                                      >  2:608265/1‑140 (MQ=255)                                                                                              cAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACAtt                                      >  2:102728/1‑140 (MQ=255)                                                                                                 aTCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGCCTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTAt                                   <  2:565503/140‑1 (MQ=255)                                                                                                   cGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCt                                 >  1:404622/1‑140 (MQ=255)                                                                                                     tCCGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGa                               >  1:877245/1‑140 (MQ=255)                                                                                                       cGGGGAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACt                             <  2:250603/140‑1 (MQ=255)                                                                                                        ggggAAACCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACtc                            <  2:79329/140‑1 (MQ=255)                                                                                                              aCCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACtcacatca                      >  2:796525/1‑140 (MQ=255)                                                                                                              aCCTTACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACtcacatca                      <  1:5299/140‑1 (MQ=255)                                                                                                                 ttACCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCacac                   <  2:300384/140‑1 (MQ=255)                                                                                                                   aCCTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACtt                 >  2:464694/1‑140 (MQ=255)                                                                                                                     cTGTCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACtttt               <  1:796525/140‑1 (MQ=255)                                                                                                                        tCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATc            <  2:714754/140‑1 (MQ=255)                                                                                                                        tCGCAAACTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATc            >  2:597987/1‑140 (MQ=255)                                                                                                                             aaCTGCGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCtt       >  2:778458/1‑140 (MQ=255)                                                                                                                                  cGACCGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTtgtg  >  1:162532/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        CCGATGCGACCGAAGCCATTTATCGCTACGCGTACGGTCATAGATCTCCTGCAAGGTTTTCCCTGAGCAAATTTGCCAGACAGAGTAATCCAGCAAATCGTCCGGGGAAACCTTACCTGTCGCAAACTGCGACTGATTGGTTAATTGTCGAACATTTAATCGACTGAAACGCTTCAGCTAGGATAAGCGAAACGTGGAATAAAAGGAATGTTTGTCCAGCCGAAGAAGACATTTATCTGACTCACATCACACTTTTATCCCCTTTTGTG  >  NC_000913/3073651‑3073919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑