Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,146,132	T→C	T44A (ACT→GCT)	hybO ←	hydrogenase 2 small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,146,132	0	T	C	100.0%	269.6 / NA	76	T44A (ACT→GCT)	hybO	hydrogenase 2 small subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/45);  total (31/45)

TTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTATCTCCAGTCATTTATA  >  NC_000913/3145998‑3146266                                                                                                                                       |                                                                                                                                       ttCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGTGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCg                                                                                                                                   >  2:373589/1‑140 (MQ=255)   cGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGAt                                                                                                                                 >  2:919309/1‑140 (MQ=255)   cGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGAt                                                                                                                                 >  1:642543/1‑140 (MQ=255)      gATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCg                                                                                                                              <  2:747842/140‑1 (MQ=255)      gATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCg                                                                                                                              <  2:206863/140‑1 (MQ=255)      gATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCg                                                                                                                              <  2:642543/140‑1 (MQ=255)           tCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCAt                                                                                                                         <  1:182566/140‑1 (MQ=255)           tCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCAt                                                                                                                         >  1:858294/1‑140 (MQ=255)             cAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCAtct                                                                                                                       >  2:872647/1‑140 (MQ=255)             cAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCAtct                                                                                                                       >  1:660912/1‑140 (MQ=255)              agagagATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCAtctc                                                                                                                      >  1:726954/1‑140 (MQ=255)               gagagaTAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATctct                                                                                                                     >  1:48293/1‑140 (MQ=255)                   gaTAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCAtct                                                                                                                       <  1:248615/134‑1 (MQ=255)                   gaTAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCAtct                                                                                                                       >  2:248615/1‑134 (MQ=255)                    aTAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGc                                                                                                                >  2:814719/1‑140 (MQ=255)                     tAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCg                                                                                                               >  1:655337/1‑140 (MQ=255)                     tAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCg                                                                                                               <  1:302326/140‑1 (MQ=255)                       gTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGc                                                                                                             >  2:878138/1‑140 (MQ=255)                         ctcCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCtt                                                                                                           <  2:858294/140‑1 (MQ=255)                          tcCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGc                                                                                                                            >  1:553019/1‑122 (MQ=255)                          tcCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGc                                                                                                                            <  2:553019/122‑1 (MQ=255)                           ccAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTg                                                                                                         <  2:275623/140‑1 (MQ=255)                            cAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGc                                                                                                        >  1:243614/1‑140 (MQ=255)                            cAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGc                                                                                                        >  2:46267/1‑140 (MQ=255)                               tACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTAc                                                                                                     >  2:209797/1‑140 (MQ=255)                                aCGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACt                                                                                                    <  1:294071/140‑1 (MQ=255)                                aCGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACt                                                                                                    <  1:872647/140‑1 (MQ=255)                                aCGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACt                                                                                                    <  1:919309/140‑1 (MQ=255)                                 cGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACtt                                                                                                   >  1:627867/1‑140 (MQ=255)                                 cGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACtt                                                                                                   >  1:14215/1‑140 (MQ=255)                                   aGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTaa                                                                                                 <  2:48293/140‑1 (MQ=255)                                    ggTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAAc                                                                                                <  1:531682/140‑1 (MQ=255)                                        ttCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCa                                                                                            <  2:421910/140‑1 (MQ=255)                                        ttCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCa                                                                                            <  2:243614/140‑1 (MQ=255)                                        ttCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCa                                                                                            <  1:878138/140‑1 (MQ=255)                                             ccGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCAtggtg                                                                                       <  1:814719/140‑1 (MQ=255)                                                ttGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTggcg                                                                                    >  2:71363/1‑140 (MQ=255)                                                          tGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAAtgctg                                                                          >  1:354832/1‑140 (MQ=255)                                                                 aGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAg                                                                   <  1:209797/140‑1 (MQ=255)                                                                      agaTTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCa                                                              >  1:866093/1‑140 (MQ=255)                                                                           ccGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGaaa                                                         >  2:291449/1‑140 (MQ=255)                                                                           ccGTACAACCAGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGaaa                                                         <  2:795273/140‑1 (MQ=255)                                                                             gTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATc                                                       >  2:51553/1‑140 (MQ=255)                                                                                cAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCacg                                                    <  1:121281/140‑1 (MQ=255)                                                                                cAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCacg                                                    <  1:327495/140‑1 (MQ=255)                                                                                cAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCacg                                                    <  1:291449/140‑1 (MQ=255)                                                                                  aCCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCacgac                                                  <  1:282462/140‑1 (MQ=255)                                                                                      gTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATc                                                       >  2:703820/1‑131 (MQ=255)                                                                                      gTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATc                                                       <  1:703820/131‑1 (MQ=255)                                                                                      gTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGtt                                              <  2:683957/140‑1 (MQ=255)                                                                                      gTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGtt                                              >  2:131184/1‑140 (MQ=255)                                                                                        gCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTaa                                            <  2:207284/140‑1 (MQ=255)                                                                                        gCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTaa                                            <  1:877609/140‑1 (MQ=255)                                                                                        gCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTaa                                            >  1:182012/1‑140 (MQ=255)                                                                                             ccTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCg                                       >  1:354811/1‑140 (MQ=255)                                                                                              cTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGt                                      <  2:182012/140‑1 (MQ=255)                                                                                                gcgcgcCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTga                                    <  2:791305/140‑1 (MQ=255)                                                                                                  gcgcCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTgaga                                  <  2:326036/140‑1 (MQ=255)                                                                                                  gcgcCAATCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTgaga                                  <  2:161534/140‑1 (MQ=255)                                                                                                       aaTCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGa                             <  2:14215/140‑1 (MQ=255)                                                                                                        aTCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGAt                            <  2:655337/140‑1 (MQ=255)                                                                                                        aTCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGAt                            >  2:101811/1‑140 (MQ=255)                                                                                                         tCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGGCGGTTAATGCCGTGAGAATGGATg                           <  2:915101/140‑1 (MQ=255)                                                                                                         tCCAGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATg                           <  2:342347/140‑1 (MQ=255)                                                                                                            aGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAgg                        <  2:441761/140‑1 (MQ=255)                                                                                                            aGATAACTGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAgg                        <  1:135032/140‑1 (MQ=255)                                                                                                                   tGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTTCTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTgaga                                  <  1:192126/123‑1 (MQ=255)                                                                                                                   tGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTgaga                                  >  2:403943/1‑123 (MQ=255)                                                                                                                   tGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTgaga                                  <  1:403943/123‑1 (MQ=255)                                                                                                                   tGGCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTgaga                                  >  2:192126/1‑123 (MQ=255)                                                                                                                     gCGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTAtct               >  2:308021/1‑140 (MQ=255)                                                                                                                      cGGACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTAtctc              <  2:726954/140‑1 (MQ=255)                                                                                                                        gACGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTATCTCCa            <  2:866093/140‑1 (MQ=255)                                                                                                                          cGCTGCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTATCTCCAGt          >  1:349555/1‑140 (MQ=255)                                                                                                                              gCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTATCTCCAGTCAtt      <  1:46267/140‑1 (MQ=255)                                                                                                                              gCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTATCTCCAGTCAtt      <  2:314468/140‑1 (MQ=255)                                                                                                                              gCGGGTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTATCTCCAGTCAtt      <  2:349555/140‑1 (MQ=255)                                                                                                                                  gTTAGCAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTATCTCCAGTCATTtata  <  2:627867/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TTCGTGATACTCCAGAGAGATAGTCTCCAGTACGAGGTTTTCTACCGTTGGATGCGTTGCACGAAGCAGAGATTCCGTACAACCGGTGCACTCCTGCGCGCCAATCCAGATAACTGGCGGACGCTGCGGGTTAGTAACCGATTCGGCCATCTCTGCAGCGGCTTTGCTACTTAACCCCATGGTGGCGGCTAATGCTGCACAAAGCTTCATGAAATCACGACGGTTAATGCCGTGAGAATGGATGAGGGTGTTATCTCCAGTCATTTATA  >  NC_000913/3145998‑3146266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑