Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,197,632	A→G	G670G (GGT→GGC)	glnE ←	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,197,632	0	A	G	100.0%	210.7 / NA	58	G670G (GGT→GGC)	glnE	fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/32);  total (26/32)

TCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCTGCGGCGATGC  >  NC_000913/3197496‑3197762                                                                                                                                         |                                                                                                                                   tcaTCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCg                                                                                                                                 >  2:165809/1‑140 (MQ=255) tcaTCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACG‑CCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGt                                                                                                                                <  1:21468/140‑1 (MQ=255)   aTCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTa                                                                                                                               <  1:165809/140‑1 (MQ=255)     cGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGc                                                                                                                             >  1:336060/1‑140 (MQ=255)        aTCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCggg                                                                                                                          <  2:585313/140‑1 (MQ=255)          ccATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCa                                                                                                                        <  1:630190/140‑1 (MQ=255)           cATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCaa                                                                                                                       <  1:51980/140‑1 (MQ=255)           cATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCaa                                                                                                                       <  1:366333/140‑1 (MQ=255)            aTTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAAc                                                                                                                      <  1:39687/140‑1 (MQ=255)                    aTCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGaa                                                                                                              >  2:133396/1‑140 (MQ=255)                    aTCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGaa                                                                                                              >  1:186665/1‑140 (MQ=255)                     tCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAAc                                                                                                             >  1:27316/1‑140 (MQ=255)                          gaggaAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACg                                                                                                        >  1:616640/1‑140 (MQ=255)                           aggaAGATAAGGTCAAGATCGGAACTGTAGCCTAACACCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGc                                                                                                       >  1:588643/1‑140 (MQ=255)                            ggaAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGcc                                                                                                      <  1:152042/140‑1 (MQ=255)                               aGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCa                                                                                                          <  2:596075/133‑1 (MQ=255)                               aGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCa                                                                                                          >  1:596075/1‑133 (MQ=255)                               aGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCctgc                                                                                                   <  1:712363/140‑1 (MQ=255)                               aGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCctgc                                                                                                   >  1:397464/1‑140 (MQ=255)                                gATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCctgct                                                                                                  >  2:150505/1‑140 (MQ=255)                                     ggTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAacg                                                                                             >  2:840560/1‑140 (MQ=255)                                      gTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAacga                                                                                            <  2:616640/140‑1 (MQ=255)                                          aGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGaa                                                                                               >  1:557537/1‑133 (MQ=255)                                          aGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGaa                                                                                               <  2:557537/133‑1 (MQ=255)                                          aGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGc                                                                                        >  2:212254/1‑140 (MQ=255)                                          aGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGc                                                                                        >  2:50437/1‑140 (MQ=255)                                          aGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGc                                                                                        <  2:397464/140‑1 (MQ=255)                                          aGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGc                                                                                        <  1:465689/140‑1 (MQ=255)                                           gATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCa                                                                                       >  2:828002/1‑140 (MQ=255)                                           gATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCa                                                                                       <  1:303336/140‑1 (MQ=255)                                           gATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCa                                                                                       >  1:19853/1‑140 (MQ=255)                                                gAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTAt                                                                                  >  2:493061/1‑140 (MQ=255)                                                  aCTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCa                                                                                <  1:493061/140‑1 (MQ=255)                                                      tAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGc                                                                            <  2:186665/140‑1 (MQ=255)                                                         ccTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTc                                                                         >  2:595858/1‑140 (MQ=255)                                                           tAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCg                                                                       >  2:840370/1‑140 (MQ=255)                                                              cTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcca                                                                    >  1:367135/1‑140 (MQ=255)                                                              cTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgcca                                                                    >  2:573917/1‑140 (MQ=255)                                                                cccAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgccagc                                                                  <  1:59043/140‑1 (MQ=255)                                                                  cAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgccagcca                                                                <  1:150505/140‑1 (MQ=255)                                                                   aGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgc                                                                      >  1:701082/1‑133 (MQ=255)                                                                   aGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCgc                                                                      <  2:701082/133‑1 (MQ=255)                                                                     ccgccCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGt                                                             <  2:142551/140‑1 (MQ=255)                                                                          cAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGt                                                        <  1:840560/140‑1 (MQ=255)                                                                          cAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGt                                                        <  1:828002/140‑1 (MQ=255)                                                                             cTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGAt                                                     <  1:266311/140‑1 (MQ=255)                                                                                gCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGc                                                  <  1:595858/140‑1 (MQ=255)                                                                                   gTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGATTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCa                                               <  2:575771/140‑1 (MQ=255)                                                                                            caccGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTtcatca                                      >  2:517952/1‑140 (MQ=255)                                                                                                gCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCgg                                  <  2:647713/140‑1 (MQ=255)                                                                                                       cACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTa                           <  2:336060/140‑1 (MQ=255)                                                                                                       cACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTa                           <  1:212254/140‑1 (MQ=255)                                                                                                                cgcgTTCGTTCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGata                  >  1:316064/1‑140 (MQ=255)                                                                                                                  cgttcgttCAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATc                <  2:19853/140‑1 (MQ=255)                                                                                                                      cgttcAGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCt            <  1:517952/140‑1 (MQ=255)                                                                                                                           aGGTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCTgcggc       <  1:50437/140‑1 (MQ=255)                                                                                                                             gTGATTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCTGCGGCGa     >  2:365285/1‑140 (MQ=255)                                                                                                                                aTTCGGCTTGCCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCTGCGGCGATGc  >  2:792015/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   TCATCGCATCCATTGGGCAATCATGGAGGAAGATAAGGTCAAGATCGGAACTGTAGCCTAACTCCCAGCCGCCCAGCTTGCCGTAGCCGACCACCGCAAAACCACGCCCTTCGCGTTCGTTCAGGTGATTCGGCTTACCGTAGCGGGCAACCATTTGAACCCACGCCTGCTGAACGACGGCATCTATCATGGCTTCCGCCAGCCAGGTTAAGTGATCGCTCACTTTCATCACCGGTAGCGTACCGGCGATATCCGCTGCGGCGATGC  >  NC_000913/3197496‑3197762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑