Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,517,260	T→A	D16V (GAT→GTT)	damX ←	cell division protein DamX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,517,260	0	T	A	100.0%	142.5 / NA	41	D16V (GAT→GTT)	damX	cell division protein DamX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (21/20);  total (21/20)

CGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCT  >  NC_000913/3517125‑3517392                                                                                                                                        |                                                                                                                                     cgcTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCt                                                                                                                                  <  2:744109/140‑1 (MQ=255)   ctctTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTgg                                                                                                                                >  2:354461/1‑140 (MQ=255)      tttttGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGAt                                                                                                                             <  2:604574/140‑1 (MQ=255)                  aGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCt                                                                                                                 <  1:899364/140‑1 (MQ=255)                  aGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCt                                                                                                                 >  2:428828/1‑140 (MQ=255)                            cGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTgg                                                                                                       >  2:69604/1‑140 (MQ=255)                              aTCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGtt                                                                                                     >  2:715092/1‑140 (MQ=255)                               tCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGttt                                                                                                    <  1:546825/140‑1 (MQ=255)                                  tCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGaa                                                                                                 >  1:50121/1‑140 (MQ=255)                                       gtCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCAt                                                                                            <  2:517862/140‑1 (MQ=255)                                          atcaAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCa                                                                                         >  1:642820/1‑140 (MQ=255)                                          atcaAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGCATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCa                                                                                         >  1:383147/1‑140 (MQ=255)                                           tcaAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCAt                                                                                        >  1:510638/1‑140 (MQ=255)                                             aaGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCAttt                                                                                      >  2:607129/1‑140 (MQ=255)                                               gTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTaa                                                                                    <  2:321206/140‑1 (MQ=255)                                                ttCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAac                                                                                   >  1:237377/1‑140 (MQ=255)                                                   aaTATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACAcc                                                                                <  2:381209/140‑1 (MQ=255)                                                     tatCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACAcccc                                                                              <  2:642820/140‑1 (MQ=255)                                                     tatCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACAcccc                                                                              >  1:72851/1‑140 (MQ=255)                                                               aTTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTaa                                                                         >  2:161236/1‑135 (MQ=255)                                                               aTTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTaa                                                                         <  1:161236/135‑1 (MQ=255)                                                               aTTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGt                                                                    <  1:354461/140‑1 (MQ=255)                                                                 tGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTa                                                                  <  1:428828/140‑1 (MQ=255)                                                                  gATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTaa                                                                 <  1:69604/140‑1 (MQ=255)                                                                   aTCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAAt                                                                >  1:203318/1‑140 (MQ=255)                                                                   aTCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGCATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAAt                                                                <  2:383147/140‑1 (MQ=255)                                                                         ggTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTAc                                                          <  2:601848/140‑1 (MQ=255)                                                                          gTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTAcc                                                         >  1:503280/1‑140 (MQ=255)                                                                                         tACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTAcc                                                         <  2:403864/125‑1 (MQ=255)                                                                                         tACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTAcc                                                         >  1:403864/1‑125 (MQ=255)                                                                                         tACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAAcc                                          >  2:778412/1‑140 (MQ=255)                                                                                            gCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGa                                       <  1:715092/140‑1 (MQ=255)                                                                                            gCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGa                                       >  1:846541/1‑140 (MQ=255)                                                                                            gCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGa                                       <  1:44769/140‑1 (MQ=255)                                                                                              tCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAg                                     >  2:31077/1‑140 (MQ=255)                                                                                                    cGTTCAGAAGATTGACGAGAACGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATa                               <  2:510638/140‑1 (MQ=255)                                                                                                                       aaCGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGcc            >  1:114675/1‑140 (MQ=255)                                                                                                                       aaCGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGcc            <  2:191928/140‑1 (MQ=255)                                                                                                                        aCGACCAGTACGACGAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCt           >  1:464348/1‑140 (MQ=255)                                                                                                                                 acgacgAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCt  <  2:203318/140‑1 (MQ=255)                                                                                                                                 acgacgAACGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCt  >  1:185204/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CGCTCTTTTTGCGCACGAGTCGGACGGCGATCGTCAGTGTCATCAAGTTCAATATCATCAAAATTGATCTGCGGTTCGCCACGTTCAGTACGCTCAGAACGTTCAGAAGATTGACGAGAACGACCAGTACGACGATCGCTGGGATCGGGTTTCAGCTCGTCTTCTGGTTTGAATTCATCCATTTAACACCCCACTAAAAGGTTAATGCTTACCACGTTGCAATTAACCTGAAGCTAATAGACCGCTTGATAAGCGGCCTGACCTTTCT  >  NC_000913/3517125‑3517392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑