Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,755,238	G→A	A425V (GCG→GTG)	aldB ←	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,755,238	0	G	A	100.0%	145.0 / NA	48	A425V (GCG→GTG)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/22);  total (26/22)

TGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCGCTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCCA  >  NC_000913/3755102‑3755370                                                                                                                                         |                                                                                                                                     tgCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCActt                                                                                                                                   >  1:274380/1‑140 (MQ=255)        tAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCAt                                                                                                                            <  1:291407/140‑1 (MQ=255)          aGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCg                                                                                                                          >  1:543920/1‑140 (MQ=255)                        tGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTc                                                                                                            <  2:296078/140‑1 (MQ=255)                               cacGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCa                                                                                                     >  2:492174/1‑140 (MQ=255)                               cacGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCa                                                                                                     >  1:223726/1‑140 (MQ=255)                                acGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAg                                                                                                    >  1:141642/1‑140 (MQ=255)                                  gcgcCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCa                                                                                                  <  2:223726/140‑1 (MQ=255)                                     cccAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCg                                                                                               >  1:148587/1‑140 (MQ=255)                                          ccTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCa                                                                                          <  1:307423/140‑1 (MQ=255)                                           cTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCaa                                                                                         <  2:141642/140‑1 (MQ=255)                                                    gcgcCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATctcct                                                                                >  1:765971/1‑140 (MQ=255)                                                     cgcCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATctcctc                                                                               >  2:369370/1‑140 (MQ=255)                                                           aTCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGaa                                                                         <  1:492174/140‑1 (MQ=255)                                                                ataGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACAccc                                                                    <  1:868312/140‑1 (MQ=255)                                                                     ccAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCata                                                               <  2:861526/140‑1 (MQ=255)                                                                      cAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCatat                                                              >  1:92660/1‑140 (MQ=255)                                                                       aGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACAccc                                                                    >  2:533117/1‑133 (MQ=255)                                                                       aGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACAccc                                                                    <  1:533117/133‑1 (MQ=255)                                                                       aGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATAtt                                                             >  2:911839/1‑140 (MQ=255)                                                                         aTTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGt                                                           <  1:88269/140‑1 (MQ=255)                                                                              cGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGa                                                      <  1:480813/140‑1 (MQ=255)                                                                              cGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGa                                                      >  2:800716/1‑140 (MQ=255)                                                                                          aGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCTAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAAtc                                          <  2:444400/140‑1 (MQ=255)                                                                                            aCGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAAtcgt                                        <  2:148587/140‑1 (MQ=255)                                                                                                   cgcCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCg                                 <  1:911839/140‑1 (MQ=255)                                                                                                   cgcCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCg                                 >  2:5521/1‑140 (MQ=255)                                                                                                    gcCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGa                                <  2:92660/140‑1 (MQ=255)                                                                                                        aGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCg                                 <  2:734900/135‑1 (MQ=255)                                                                                                        aGGCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCg                                 >  1:734900/1‑135 (MQ=255)                                                                                                          gCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGgtagt                          <  2:536916/140‑1 (MQ=255)                                                                                                          gCCATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGgtagt                          >  1:196176/1‑140 (MQ=255)                                                                                                            cATATTGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGtagtag                        <  1:369370/140‑1 (MQ=255)                                                                                                               tttGCGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCg                     <  1:231063/139‑1 (MQ=255)                                                                                                                   cGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGtt                                   <  1:220106/122‑1 (MQ=255)                                                                                                                   cGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGtt                                   >  2:220106/1‑122 (MQ=255)                                                                                                                   cGTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCtt                 >  2:47123/1‑140 (MQ=255)                                                                                                                    gTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCttt                >  2:744208/1‑140 (MQ=255)                                                                                                                    gTATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCttt                >  2:767069/1‑140 (MQ=255)                                                                                                                     tATCGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTc               >  2:853908/1‑140 (MQ=255)                                                                                                                        cGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCttt                >  2:300949/1‑136 (MQ=255)                                                                                                                        cGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCttt                <  1:300949/136‑1 (MQ=255)                                                                                                                        cGTTCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGt            >  2:865913/1‑140 (MQ=255)                                                                                                                          ttCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTc          >  2:152629/1‑140 (MQ=255)                                                                                                                           tCGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCtttcatttca         >  1:423358/1‑140 (MQ=255)                                                                                                                            cGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGgtagt                          <  2:33057/122‑1 (MQ=255)                                                                                                                            cGCCAGCTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGgtagt                          >  1:33057/1‑122 (MQ=255)                                                                                                                                  cTCCAGCACTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCACATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCca  >  2:133712/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCCGCGCCCCATCTTATAGGCCAGATTACCGTTGCGGCTCCAGACGCCCGCGCCCAGGCCATATTGCGTATCGTTCGCCAGCTCCAGCGCTTCTTCCATCGTTTTGAAGGTGGTCACCGCCAGCACCGGGCCAAAAATCTCCTCCTGGAACACCCGCATATTGTTCTGACCAAACAGAATCGTCGGTTCGAGGTAGTAGCCGTCTTTCAGTTCACCTTCCA  >  NC_000913/3755102‑3755370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑