Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,259	A→G	L66P (CTT→CCT)	mioC ←	flavoprotein MioC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,259	0	A	G	100.0%	158.2 / NA	45	L66P (CTT→CCT)	mioC	flavoprotein MioC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/25);  total (20/25)

TTGAGTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGT  >  NC_000913/3926127‑3926394                                                                                                                                     |                                                                                                                                        ttGAGTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTc                                                                                                                                  >  1:120624/1‑140 (MQ=255)  tGAGTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTcc                                                                                                                                 <  2:597604/140‑1 (MQ=255)   gAGTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCg                                                                                                                                <  1:687269/140‑1 (MQ=255)     gTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGa                                                                                                                              >  2:762992/1‑140 (MQ=255)     gTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGa                                                                                                                              <  2:114780/140‑1 (MQ=255)       tCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAat                                                                                                                            <  1:89595/140‑1 (MQ=255)       tCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAat                                                                                                                            <  1:635712/140‑1 (MQ=255)        cTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAata                                                                                                                           >  2:672691/1‑140 (MQ=255)         tGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAatat                                                                                                                          <  2:569164/140‑1 (MQ=255)         tGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAatat                                                                                                                          <  2:159876/140‑1 (MQ=255)           ccTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATAtct                                                                                                                        <  2:274722/140‑1 (MQ=255)             tCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTcc                                                                                                                      <  1:825350/140‑1 (MQ=255)                aGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGc                                                                                                                   <  2:301648/140‑1 (MQ=255)                   ttATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCAcc                                                                                                                <  1:176107/140‑1 (MQ=255)                   ttATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCAcc                                                                                                                <  1:151714/140‑1 (MQ=255)                   ttATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCAcc                                                                                                                >  2:176107/1‑140 (MQ=255)                     aTCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCgt                                                                                                              >  2:901479/1‑140 (MQ=255)                           aGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCgtgggtgg                                                                                                        >  2:83343/1‑140 (MQ=255)                               ccACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCt                                                                                                    <  2:62134/140‑1 (MQ=255)                                cacaAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTg                                                                                                   >  1:306593/1‑140 (MQ=255)                                  caAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGAt                                                                                                 <  1:893139/140‑1 (MQ=255)                                        gtgtCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGTAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAccag                                                                                           >  1:321052/1‑140 (MQ=255)                                          gtCATA‑TCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAccagcca                                                                                        <  1:196831/140‑1 (MQ=255)                                           tCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAAccagcca                                                                                        <  1:58125/140‑1 (MQ=255)                                            cATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGAt                                                                                                 <  2:419429/130‑1 (MQ=255)                                            cATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGAt                                                                                                 >  1:419429/1‑130 (MQ=255)                                                        tGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCa                                                                           >  2:403307/1‑140 (MQ=255)                                                         gCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAg                                                                          >  2:79494/1‑140 (MQ=255)                                                          ccAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAgg                                                                         <  1:672691/140‑1 (MQ=255)                                                          ccAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAgg                                                                         >  2:61035/1‑140 (MQ=255)                                                          ccAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAgg                                                                         >  2:390034/1‑140 (MQ=255)                                                            aaTACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTa                                                                       <  1:875537/140‑1 (MQ=255)                                                            aaTACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTa                                                                       <  1:79494/140‑1 (MQ=255)                                                            aaTACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTa                                                                       <  1:13121/140‑1 (MQ=255)                                                                ccGATTGCGCCAAAGCGGACTGTAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAAtc                                                                   <  2:321052/140‑1 (MQ=255)                                                                   aTTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAAtcttc                                                                >  1:393839/1‑140 (MQ=255)                                                                   aTTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAAtcttc                                                                <  1:311921/140‑1 (MQ=255)                                                                    ttGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAAtcttct                                                               >  2:178012/1‑140 (MQ=255)                                                                             aGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGa                                                      <  1:178012/140‑1 (MQ=255)                                                                                                gggCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCggtgg                                   >  1:780530/1‑140 (MQ=255)                                                                                                      cTGTTCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAAc                             >  2:424336/1‑140 (MQ=255)                                                                                                          tCCTGCAATGCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGc                         >  1:746797/1‑140 (MQ=255)                                                                                                                  acagtCATAGAAAGGAGAAGGGTTGTCCGGAATACCTCCGGCCCGGTGGGTGGAGCTGATAACCAGCAAGACCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCATGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCa                 <  2:775519/136‑1 (MQ=255)                                                                                                                   gCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAg                >  2:300319/1‑140 (MQ=255)                                                                                                                   gCTTCATAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAg                >  1:525598/1‑140 (MQ=255)                                                                                                                         tAGAAAGGAGAAGGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTc          <  1:723301/140‑1 (MQ=255)                                                                                                                          agaaaggagaggggTTGTCCGGAATTTCCCCGCCACCGTGGGGGGGGCTGATACCCGCCCAGTTCCCTGAGGAGGGTAAATCTTCTAACAGCGGACCGTGCAGGGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCa         <  2:393839/140‑1 (MQ=255)                                                                                                                              aggagaagggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAACCCCGCCTCTTCCAGCTTTTCAGCCa     <  2:120624/140‑1 (MQ=255)                                                                                                                              aggagaagggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCa     >  2:300799/1‑140 (MQ=255)                                                                                                                              aggagaagggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCa     >  1:474241/1‑140 (MQ=255)                                                                                                                              aggagaagggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCa     >  1:61979/1‑140 (MQ=255)                                                                                                                               ggagaagggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCGGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAg    <  2:306593/140‑1 (MQ=255)                                                                                                                                 agaagggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCTGgt  >  2:237757/1‑140 (MQ=255)                                                                                                                                 agaagggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCATGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAgacctgt  >  1:775519/1‑134 (MQ=255)                                                                                                                                     |                                                                                                                                        TTGAGTTCTGCCTCGAGTTTATCGATAGCCCCACAAAAGGTGTCATATTCACGACTGCCAATACCGATTGCGCCAAAGCGGACTGCAGAAAGATCGGGCTTCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTCGGTGGTAAAACCCGCCTCTTCCAGCTTTTCAGCCAGGT  >  NC_000913/3926127‑3926394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑