Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,032,131	T→C	V324A (GTC→GCC)	pepQ →	Xaa‑Pro dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,032,131	0	T	C	100.0%	155.3 / NA	44	V324A (GTC→GCC)	pepQ	Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/18);  total (26/18)

ATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTAC  >  NC_000913/4031996‑4032264                                                                                                                                        |                                                                                                                                      atgaACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGaa                                                                                                                                   <  2:1933/140‑1 (MQ=255)   gaACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGaaaa                                                                                                                                 <  1:394955/140‑1 (MQ=255)                tGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTGCCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCggg                                                                                                                    <  2:544285/140‑1 (MQ=255)                tGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCggg                                                                                                                    <  2:741817/140‑1 (MQ=255)                            tGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCg                                                                                                        >  1:650948/1‑140 (MQ=255)                              aaaGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCcaca                                                                                                      <  2:167632/140‑1 (MQ=255)                              aaaGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCa                                                                                                      <  2:364872/140‑1 (MQ=255)                                    ggCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTAt                                                                                                >  2:223214/1‑140 (MQ=255)                                               tgtgGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGc                                                                                     >  2:178789/1‑140 (MQ=255)                                                gtgGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCt                                                                                    <  2:650948/140‑1 (MQ=255)                                                      tACCACATCCAGTTCCATCAGCGCATCGCCAAACTGTTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCg                                                                                      <  1:757482/132‑1 (MQ=255)                                                      tACCACATCCAGTTCCATCAGCGCATCGCCAAACTGTTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCg                                                                                      >  2:757482/1‑132 (MQ=255)                                                      tACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCt                                                                              >  1:508962/1‑140 (MQ=255)                                                       aCCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTg                                                                             >  1:750984/1‑140 (MQ=255)                                                       aCCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTACCGGCCATCCGCTGGGCCTg                                                                             >  2:867246/1‑140 (MQ=255)                                                         cacaTCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCa                                                                           >  1:57284/1‑140 (MQ=255)                                                          acaTCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAg                                                                          <  2:750984/140‑1 (MQ=255)                                                             tCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTg                                                                       >  1:515639/1‑140 (MQ=255)                                                             tCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTg                                                                       >  2:350124/1‑140 (MQ=255)                                                              ccAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGc                                                                      >  1:108410/1‑140 (MQ=255)                                                                       tCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCg                                                             >  1:762662/1‑140 (MQ=255)                                                                          gcgcATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGc                                                            <  2:382243/138‑1 (MQ=255)                                                                          gcgcATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGc                                                            >  1:382243/1‑138 (MQ=255)                                                                          gcgcATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTg                                                          >  2:298754/1‑140 (MQ=255)                                                                            gcATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGt                                                        >  2:610952/1‑140 (MQ=255)                                                                                  ccAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTCCCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATg                                                  <  1:178789/140‑1 (MQ=255)                                                                                  ccAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATg                                                  <  1:223214/140‑1 (MQ=255)                                                                                  ccAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATg                                                  <  1:298754/140‑1 (MQ=255)                                                                                     aaCTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAg                                               <  1:19427/140‑1 (MQ=255)                                                                                     aaCTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAg                                               <  1:610952/140‑1 (MQ=255)                                                                                          ctgcGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGgatga                                          >  2:260712/1‑140 (MQ=255)                                                                                          ctgcGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGgatga                                          >  1:675542/1‑140 (MQ=255)                                                                                          ctgcGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCT‑GTTTTATGCAGgatgat                                         >  1:765163/1‑140 (MQ=255)                                                                                             cGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAg                                       >  2:452729/1‑140 (MQ=255)                                                                                                      cAAATCATCACCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCa                              <  1:452729/140‑1 (MQ=255)                                                                                                          tcatcaCCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTc                          <  1:260712/140‑1 (MQ=255)                                                                                                              caCCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCgg                      >  1:526151/1‑140 (MQ=255)                                                                                                              caCCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCgg                      >  1:523934/1‑140 (MQ=255)                                                                                                              caCCGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCgg                      >  2:805428/1‑140 (MQ=255)                                                                                                                 cGATATGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCAc                   <  2:162301/140‑1 (MQ=255)                                                                                                                      tGAGTGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCa              >  1:620612/1‑140 (MQ=255)                                                                                                                          tGAAGAAGCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAat          >  2:255856/1‑140 (MQ=255)                                                                                                                                 gCGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTa   >  1:442536/1‑140 (MQ=255)                                                                                                                                  cGATGGCCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTAc  >  2:385970/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      ATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTAC  >  NC_000913/4031996‑4032264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑