Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,075,441	A→G	Q258R (CAG→CGG)	yihW →	putative DNA‑binding transcriptional regulator YihW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,075,441	0	A	G	100.0%	181.9 / NA	51	Q258R (CAG→CGG)	yihW	putative DNA‑binding transcriptional regulator YihW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/28);  total (23/28)

CACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGT  >  NC_000913/4075307‑4075576                                                                                                                                       |                                                                                                                                        cacTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAg                                                                                                                                    >  2:156950/1‑140 (MQ=255) cacTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAg                                                                                                                                    <  2:612942/140‑1 (MQ=255)         aTCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAg                                                                                                                            <  1:10317/140‑1 (MQ=255)            aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGAtt                                                                                                                         >  2:475489/1‑140 (MQ=255)            aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGAtt                                                                                                                         >  2:503448/1‑140 (MQ=255)                ttCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTtg                                                                                                                     >  2:762247/1‑140 (MQ=255)                 tCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTtgt                                                                                                                    >  1:800176/1‑140 (MQ=255)                  cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTtgtg                                                                                                                   >  2:601472/1‑140 (MQ=255)                  cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTtgtg                                                                                                                   <  1:503448/140‑1 (MQ=255)                  cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTtgtg                                                                                                                   >  1:520349/1‑140 (MQ=255)                  cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTtgtg                                                                                                                   >  2:300914/1‑140 (MQ=255)                  cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTtgtg                                                                                                                   >  2:202955/1‑140 (MQ=255)                  cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTtgtg                                                                                                                   >  1:104109/1‑140 (MQ=255)                   ggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGa                                                                                                                  >  2:667814/1‑140 (MQ=255)                          gTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTaa                                                                                                           >  2:35472/1‑140 (MQ=255)                            tGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAAcc                                                                                                         <  2:416282/140‑1 (MQ=255)                             gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCt                                                                                                        <  2:520349/140‑1 (MQ=255)                                     tAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCt                                                                                                        <  1:807331/132‑1 (MQ=255)                                     tAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCt                                                                                                        >  2:807331/1‑132 (MQ=255)                                          tGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCtt                                                                                           <  1:6773/140‑1 (MQ=255)                                                aGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcaca                                                                                     <  2:800176/140‑1 (MQ=255)                                                           ctctTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAAccc                                                                          <  1:762247/140‑1 (MQ=255)                                                            tctTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAGATCATTCTTCCCCGGGAAGACGCGAAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCa                                                                         >  2:561432/1‑140 (MQ=255)                                                              tttACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcaca                                                                                     <  1:118505/126‑1 (MQ=255)                                                              tttACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcaca                                                                                     >  2:118505/1‑126 (MQ=255)                                                              tttACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATc                                                                       >  1:326667/1‑140 (MQ=255)                                                               ttACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATcc                                                                      <  2:326667/140‑1 (MQ=255)                                                                tACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCt                                                                     >  2:507838/1‑140 (MQ=255)                                                                    gacgaGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTcc                                                                 <  2:715781/140‑1 (MQ=255)                                                                        aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCt                                                             >  2:488028/1‑140 (MQ=255)                                                                                        gcTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtgt                                             <  1:507838/140‑1 (MQ=255)                                                                                                 aCGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGaaa                                    >  2:799971/1‑140 (MQ=255)                                                                                                 aCGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGaaa                                    <  1:475489/140‑1 (MQ=255)                                                                                                    cTTACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAagga                                 <  2:104109/140‑1 (MQ=255)                                                                                                      tACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                               >  2:344247/1‑140 (MQ=255)                                                                                                      tACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                               <  1:601472/140‑1 (MQ=255)                                                                                                      tACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCATTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                               >  2:768558/1‑140 (MQ=255)                                                                                                      tACAAGACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGAAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                               <  1:561432/140‑1 (MQ=255)                                                                                                           gACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaa                          <  1:667814/140‑1 (MQ=255)                                                                                                           gACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaa                          <  1:202955/140‑1 (MQ=255)                                                                                                           gACAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaa                          <  1:177499/140‑1 (MQ=255)                                                                                                            aCAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaaa                         <  1:882259/140‑1 (MQ=255)                                                                                                            aCAGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaaa                         <  1:768558/140‑1 (MQ=255)                                                                                                              aGCCAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATg                       <  1:35472/140‑1 (MQ=255)                                                                                                                 cAAATTGAAATCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctc                    >  1:341718/1‑140 (MQ=255)                                                                                                                        aaaTCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt             <  1:344247/140‑1 (MQ=255)                                                                                                                          aTCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt             >  2:670527/1‑138 (MQ=255)                                                                                                                          aTCATTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt             <  1:670527/138‑1 (MQ=255)                                                                                                                             aTTCTTCCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGAt        <  1:292675/140‑1 (MQ=255)                                                                                                                              ttcttcCCCGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGAtt       <  1:799971/140‑1 (MQ=255)                                                                                                                                   ccccGGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGt  <  2:496007/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CACTAAGTATCATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGT  >  NC_000913/4075307‑4075576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑