Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,124,105	A→G	L118L (TTG→CTG)	cytR ←	DNA‑binding transcriptional repressor CytR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,124,105	0	A	G	100.0%	176.6 / NA	49	L118L (TTG→CTG)	cytR	DNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

AGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAAT  >  NC_000913/4123971‑4124240                                                                                                                                       |                                                                                                                                        aGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGa                                                                                                                                    >  1:313643/1‑140 (MQ=255)  ggCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGAt                                                                                                                                   >  2:434926/1‑140 (MQ=255)     agctccagctccGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATaaa                                                                                                                                >  2:557243/1‑140 (MQ=255)       ctccagctccGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAgg                                                                                                                              <  2:719340/140‑1 (MQ=255)        tccagctccGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGt                                                                                                                             >  2:426314/1‑140 (MQ=255)                 ggTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCctg                                                                                                                    >  2:680598/1‑140 (MQ=255)                  gTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCctgc                                                                                                                   <  2:313643/140‑1 (MQ=255)                  gTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGACCGATAAAGGTTTTTTCctgc                                                                                                                   <  2:908780/140‑1 (MQ=255)                                       ccATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCg                                                                                              <  1:906671/140‑1 (MQ=255)                                         aTCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAA‑AACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCa                                                                                           <  2:809511/140‑1 (MQ=255)                                            ggcggcAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAAt                                                                                         <  2:694501/140‑1 (MQ=255)                                                 cAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCa                                                                                    >  1:378682/1‑140 (MQ=255)                                                      tACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGa                                                                               <  1:310918/140‑1 (MQ=255)                                                             ttCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGt                                                                        >  1:266383/1‑140 (MQ=255)                                                              tCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTg                                                                       >  1:125555/1‑140 (MQ=255)                                                              tCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTg                                                                       >  1:258147/1‑140 (MQ=255)                                                               ccTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGa                                                                      >  1:905110/1‑140 (MQ=255)                                                                  cAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGAttt                                                                   <  1:557243/140‑1 (MQ=255)                                                                  cAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGAttt                                                                   <  2:235686/140‑1 (MQ=255)                                                                   aaTGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTg                                                                  >  1:451889/1‑140 (MQ=255)                                                                   aaTGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTg                                                                  <  2:212432/140‑1 (MQ=255)                                                                       cTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTgccgc                                                              >  1:542481/1‑140 (MQ=255)                                                                          gCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGt                                                           <  2:703138/140‑1 (MQ=255)                                                                          gCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGt                                                           <  1:241093/140‑1 (MQ=255)                                                                          gCATC‑AACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTa                                                          >  2:908179/1‑140 (MQ=255)                                                                            accAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTaa                                                         <  2:850264/138‑1 (MQ=255)                                                                               aaaCGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTgccgcc                                                             >  1:454006/1‑133 (MQ=255)                                                                               aaaCGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTgccgcc                                                             <  2:454006/133‑1 (MQ=255)                                                                                 aCGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCg                                                    <  2:687925/140‑1 (MQ=255)                                                                                   ggCAGCCTTGACACCAGCAACAACATGCAATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACGCTCGCCAATCAGCCCCAGATATCCGTGATTTGCCGCCGTAACTTCGAt                                                  <  2:542481/140‑1 (MQ=255)                                                                                    gCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATa                                                 <  1:908179/140‑1 (MQ=255)                                                                                        ccTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACcgc                                             >  1:422460/1‑140 (MQ=255)                                                                                        ccTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACcgc                                             >  2:740925/1‑140 (MQ=255)                                                                                             aaCCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGaata                                        >  1:35057/1‑140 (MQ=255)                                                                                               cccAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCACACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGaataat                                      <  2:731346/140‑1 (MQ=255)                                                                                                  aGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTc                                   >  2:528899/1‑140 (MQ=255)                                                                                                  aGCAACAACATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTc                                   >  2:847703/1‑140 (MQ=255)                                                                                                    caacaaCATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGc                                 <  2:125555/140‑1 (MQ=255)                                                                                                     aacaacATGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCt                                <  2:905110/140‑1 (MQ=255)                                                                                                       caacaTGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTaa                              >  2:465314/1‑140 (MQ=255)                                                                                                          caTGCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAaaga                           <  2:86440/140‑1 (MQ=255)                                                                                                             gCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAgg                        <  2:451889/140‑1 (MQ=255)                                                                                                             gCCATCAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAgg                        <  1:680598/140‑1 (MQ=255)                                                                                                                  cAATTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCg                   >  2:435857/1‑140 (MQ=255)                                                                                                                   aaTTTGCTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGc                  <  2:35057/140‑1 (MQ=255)                                                                                                                         cTTGGTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGatat            >  2:321492/1‑140 (MQ=255)                                                                                                                             gTGATGATCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCgg        >  2:269801/1‑140 (MQ=255)                                                                                                                              tgatgaTCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCggg       <  2:422460/140‑1 (MQ=255)                                                                                                                                atgatCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGAc     <  2:113461/140‑1 (MQ=255)                                                                                                                                   atCAGATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAAt  <  1:269801/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AGGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAAT  >  NC_000913/4123971‑4124240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑