Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	405,536	G→T	P37T (CCC→ACC)	proC ←	pyrroline‑5‑carboxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	405,536	0	G	T	100.0%	144.0 / NA	43	P37T (CCC→ACC)	proC	pyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/22);  total (21/22)

GCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGGGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCATTGCCTCACTCCTGCCGTGAA  >  NC_000913/405402‑405664                                                                                                                                       |                                                                                                                                 gCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGgtgt                                                                                                                             <  1:618499/140‑1 (MQ=255)      gCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATAcc                                                                                                                        <  2:717858/140‑1 (MQ=255)          tttAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGa                                                                                                                    <  2:220557/140‑1 (MQ=255)            tAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGAtt                                                                                                                  >  1:78611/1‑140 (MQ=255)            tAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGAtt                                                                                                                  <  2:827210/140‑1 (MQ=255)                    atgCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTgg                                                                                                          >  1:771679/1‑140 (MQ=255)                      gCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTgg                                                                                                          >  2:664677/1‑138 (MQ=255)                      gCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTgg                                                                                                          <  1:664677/138‑1 (MQ=255)                               aaCGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGAc                                                                                               >  1:174236/1‑140 (MQ=255)                               aaCGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGAc                                                                                               <  2:247205/140‑1 (MQ=255)                               aaCGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGAc                                                                                               <  2:771679/140‑1 (MQ=255)                               aaCGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGAc                                                                                               <  2:842204/140‑1 (MQ=255)                                    cagcaAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGAc                                                                                               <  2:695517/135‑1 (MQ=255)                                    cagcaAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGAc                                                                                               >  1:695517/1‑135 (MQ=255)                                    cagcaAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTg                                                                                          >  2:769091/1‑140 (MQ=255)                                    cagcaAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTg                                                                                          >  1:680622/1‑140 (MQ=255)                                      gcaAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGc                                                                                        <  2:163603/140‑1 (MQ=255)                                             tgatgTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGa                                                                                 <  1:818634/140‑1 (MQ=255)                                                tgTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGAccg                                                                              <  1:780006/140‑1 (MQ=255)                                                  tCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGAcc                                                                               <  2:867588/137‑1 (MQ=255)                                                  tCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGAcc                                                                               >  1:867588/1‑137 (MQ=255)                                                          ttGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATgg                                                                    <  1:728376/140‑1 (MQ=255)                                                           tGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGc                                                                   <  2:78611/140‑1 (MQ=255)                                                            gcgcCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCt                                                                  >  1:835097/1‑140 (MQ=255)                                                                cACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTc                                                              <  1:769091/140‑1 (MQ=255)                                                                cACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTc                                                              <  2:486484/140‑1 (MQ=255)                                                                  cttcttGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTccc                                                            >  1:675602/1‑140 (MQ=255)                                                                     cttGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCata                                                         >  2:635298/1‑140 (MQ=255)                                                                     cttGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCata                                                         <  2:835097/140‑1 (MQ=255)                                                                            cGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGc                                                  >  2:531644/1‑140 (MQ=255)                                                                               ttCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGc                                               >  1:533891/1‑140 (MQ=255)                                                                                    cggcgTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTg                                                      <  2:464493/128‑1 (MQ=255)                                                                                    cggcgTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTg                                                      >  1:464493/1‑128 (MQ=255)                                                                                        gTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAAc                                      >  1:37969/1‑140 (MQ=255)                                                                                            aTGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGAt                                  <  2:420531/140‑1 (MQ=255)                                                                                                       gTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCa                       <  2:22291/140‑1 (MQ=255)                                                                                                       gTCATGCAGGGCGGCGACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCa                       <  1:635298/140‑1 (MQ=255)                                                                                                                 gcggcgACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCATTGCCTCACt             >  1:666643/1‑140 (MQ=255)                                                                                                                  cggcgACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCATTGCCTCACTc            >  2:346631/1‑140 (MQ=255)                                                                                                                  cggcgACTTTATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCATTGCCTCACTc            >  2:152712/1‑140 (MQ=255)                                                                                                                          ttATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCATTGCCTCACTCCTGCCGTg    >  1:704947/1‑140 (MQ=255)                                                                                                                           tATCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCATTGCCTCACTCCTGCCGTGa   >  1:884201/1‑140 (MQ=255)                                                                                                                            aTCCGGGGAGGTGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCATTGCCTCACTCCTGCCGTGaa  >  1:589693/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                 GCTAAGCACTTTAATCATGATGCCAGGTTTAACGGCAGCAAAAATGATGTCGGCGATTTGCGCCACTTCTTGCGCCGATTCTGCGGCGTTGATGCCGAACTGGTCATGCAGGGCGGCGACTTTATCCGGGGAGGGGGTGTATACCCAGATTTGCCCTGGAAGCACCTGACCGCTGGCAATCAGACCGCCGAGAATGGCTTTTCCCATATTGCCGCAGCCAATAAAACCGATTTTCTTTTCCATTGCCTCACTCCTGCCGTGAA  >  NC_000913/405402‑405664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑