Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,440,531	C→T	E229K (GAA→AAA)	ytfL ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,440,531	0	C	T	100.0%	253.8 / NA	72	E229K (GAA→AAA)	ytfL	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/35);  total (37/35)

CTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACT  >  NC_000913/4440395‑4440667                                                                                                                                         |                                                                                                                                         cTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTtgtg                                                                                                                                       <  1:654512/140‑1 (MQ=255)    tGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGgag                                                                                                                                    >  2:758218/1‑140 (MQ=255)    tGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGgag                                                                                                                                    >  1:849061/1‑140 (MQ=255)               cACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGCGGAGATCAAACCaaa                                                                                                                         >  2:710775/1‑140 (MQ=255)                            agGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCAcg                                                                                                            <  1:907717/140‑1 (MQ=255)                            agGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCAcg                                                                                                            <  2:162892/140‑1 (MQ=255)                            agGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCAcg                                                                                                            <  2:618210/140‑1 (MQ=255)                            agGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGCGGAGATCAAACCAAATCACGTTTTCAcg                                                                                                            <  1:710775/140‑1 (MQ=255)                            agGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCAACTTTGTGGAGATCAAACCAAATCACGTTTTCAcg                                                                                                            >  2:497139/1‑140 (MQ=255)                                cTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGgt                                                                                                        >  2:238761/1‑140 (MQ=255)                                cTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGgt                                                                                                        >  1:518203/1‑140 (MQ=255)                                cTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGgt                                                                                                        >  2:727342/1‑140 (MQ=255)                                 tttAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGgtg                                                                                                       <  2:340160/140‑1 (MQ=255)                                        tCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGa                                                                                                >  1:799403/1‑140 (MQ=255)                                        tCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCACCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGa                                                                                                >  1:295358/1‑140 (MQ=255)                                        tCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAAATTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGa                                                                                                >  1:618859/1‑140 (MQ=255)                                          gACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAg                                                                                              >  1:19865/1‑140 (MQ=255)                                                aCCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGaa                                                                                        <  1:497139/140‑1 (MQ=255)                                                aCCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGaa                                                                                        <  1:274763/140‑1 (MQ=255)                                                    atgatgTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGt                                                                                    >  1:660808/1‑140 (MQ=255)                                                      gatgTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTAc                                                                                  >  1:584170/1‑140 (MQ=255)                                                          tgGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGa                                                                              >  2:187701/1‑140 (MQ=255)                                                            gTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGAtt                                                                            >  1:72466/1‑140 (MQ=255)                                                             tCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGa                                                                                         <  1:850773/126‑1 (MQ=255)                                                             tCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGa                                                                                         >  2:850773/1‑126 (MQ=255)                                                             tCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTc                                                                           <  2:584170/140‑1 (MQ=255)                                                               aaTATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCa                                                                         <  2:849061/140‑1 (MQ=255)                                                                  atCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCt                                                                      <  2:19865/140‑1 (MQ=255)                                                                  atCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCCTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCt                                                                      >  1:66138/1‑140 (MQ=255)                                                                    cTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTa                                                                                   <  1:626738/125‑1 (MQ=255)                                                                    cTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTa                                                                                   >  2:626738/1‑125 (MQ=255)                                                                      tCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCaaa                                                                  <  2:491886/140‑1 (MQ=255)                                                                      tCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCaaa                                                                  >  1:82408/1‑140 (MQ=255)                                                                       cATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCa                                                                    >  1:729341/1‑137 (MQ=255)                                                                       cATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCa                                                                    <  2:729341/137‑1 (MQ=255)                                                                       cATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAg                                                                 >  2:758597/1‑140 (MQ=255)                                                                           aCAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTc                                                                           <  2:392325/126‑1 (MQ=255)                                                                           aCAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTc                                                                           >  1:392325/1‑126 (MQ=255)                                                                            cAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtt                                                            <  1:758597/140‑1 (MQ=255)                                                                            cAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtt                                                            <  1:758218/140‑1 (MQ=255)                                                                            cAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtt                                                            <  1:352869/140‑1 (MQ=255)                                                                              gACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtttt                                                          <  1:136099/140‑1 (MQ=255)                                                                              gACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtttt                                                          <  2:72466/140‑1 (MQ=255)                                                                               aCGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAGGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTc                                                         >  2:311849/1‑140 (MQ=255)                                                                                    gAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCTTCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCa                                                    <  2:439727/140‑1 (MQ=255)                                                                                    gAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCa                                                    >  2:636143/1‑140 (MQ=255)                                                                                    gAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTCCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCa                                                    >  1:626026/1‑140 (MQ=255)                                                                                      aCTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGTTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                                                  <  1:327368/140‑1 (MQ=255)                                                                                      aCTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                                                  <  1:727342/140‑1 (MQ=255)                                                                                       cTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCt                                                 <  2:104947/140‑1 (MQ=255)                                                                                            agTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCgtgt                                            >  2:233560/1‑140 (MQ=255)                                                                                              tGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTc                                          <  2:799403/140‑1 (MQ=255)                                                                                                cGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCt                                        <  1:187701/140‑1 (MQ=255)                                                                                                cGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCt                                        >  1:570509/1‑140 (MQ=255)                                                                                                    tGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAGGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAACCAGCTCGTGTTCCTGttt                                    <  1:311849/140‑1 (MQ=255)                                                                                                      ttCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTAc                                  >  2:799673/1‑140 (MQ=255)                                                                                                            cACCTTATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAaca                            <  1:636143/140‑1 (MQ=255)                                                                                                                 tATTCTTCAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACAcgccc                       >  1:21552/1‑140 (MQ=255)                                                                                                                   ttcttcAGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACAcgcccgc                     <  2:880570/140‑1 (MQ=255)                                                                                                                         aGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAgcgc               >  1:520500/1‑140 (MQ=255)                                                                                                                         aGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAgcgc               >  2:94041/1‑140 (MQ=255)                                                                                                                         aGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAgcgc               >  2:90985/1‑140 (MQ=255)                                                                                                                         aGGCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAgcgc               >  1:542365/1‑140 (MQ=255)                                                                                                                          ggCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTGCTGTTTACGTAACACGCCCGCCAGCGCa              >  1:505344/1‑140 (MQ=255)                                                                                                                          ggCTTTGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCa              <  2:520500/140‑1 (MQ=255)                                                                                                                             tttGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCg           <  2:660808/140‑1 (MQ=255)                                                                                                                               tGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGc         >  2:837090/1‑140 (MQ=255)                                                                                                                               tGCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGc         <  1:238761/140‑1 (MQ=255)                                                                                                                                gCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCAc             <  2:659443/135‑1 (MQ=255)                                                                                                                                gCTCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCAc             >  1:659443/1‑135 (MQ=255)                                                                                                                                  tCATCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTc      <  1:799673/140‑1 (MQ=255)                                                                                                                                     tCTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCAc   <  2:21552/140‑1 (MQ=255)                                                                                                                                      cTTTGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACt  >  1:520713/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACT  >  NC_000913/4440395‑4440667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑