Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,445,549	A→G	T480A (ACC→GCC)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,445,549	0	A	G	100.0%	201.1 / NA	56	T480A (ACC→GCC)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/32);  total (24/32)

GCTCGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGG  >  NC_000913/4445416‑4445680                                                                                                                                      |                                                                                                                                    gCTCGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGc                                                                                                                               >  1:122175/1‑140 (MQ=255)      aCTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATg                                                                                                                          >  2:378266/1‑140 (MQ=255)             aCAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGc                                                                                                                   <  2:43150/140‑1 (MQ=255)              cAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGcc                                                                                                                  <  1:177493/140‑1 (MQ=255)               aGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCg                                                                                                                 <  2:595871/140‑1 (MQ=255)                 gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGc                                                                                                                   >  2:881710/1‑136 (MQ=255)                 gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGc                                                                                                                   <  1:881710/136‑1 (MQ=255)                 gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGa                                                                                                               >  2:412863/1‑140 (MQ=255)                    aTTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGtt                                                                                                            >  1:552482/1‑140 (MQ=255)                           ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                     >  1:817139/1‑140 (MQ=255)                           ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                     >  1:679102/1‑140 (MQ=255)                           ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                     >  1:70573/1‑140 (MQ=255)                             cgcgGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGAc                                                                                                   >  2:439618/1‑140 (MQ=255)                               cgGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCg                                                                                                 >  1:313546/1‑140 (MQ=255)                                     gCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACg                                                                                           <  1:160116/140‑1 (MQ=255)                                          cGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTaaa                                                                                      <  2:70573/140‑1 (MQ=255)                                              tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGa                                                                                  >  1:922021/1‑140 (MQ=255)                                              tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGCCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGa                                                                                  >  1:311381/1‑140 (MQ=255)                                               aaCGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGaa                                                                                 <  1:378266/140‑1 (MQ=255)                                               aaCGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGaa                                                                                 <  2:202453/140‑1 (MQ=255)                                                              gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGa                                                                  >  2:13975/1‑140 (MQ=255)                                                               ccAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGAc                                                                 >  2:755934/1‑140 (MQ=255)                                                                         cccGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGa                                                       <  1:439618/140‑1 (MQ=255)                                                                              aCTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                  <  2:857422/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:439195/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:922021/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:504616/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:313546/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:552482/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:679102/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:173594/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  1:911840/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  1:412863/140‑1 (MQ=255)                                                                                tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACATTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:817139/140‑1 (MQ=255)                                                                                tGGCCGCCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:311381/140‑1 (MQ=255)                                                                                  gCCGTCGAAACTCAGTGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                              >  2:86811/1‑140 (MQ=255)                                                                                  gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  1:739513/138‑1 (MQ=255)                                                                                  gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                >  2:739513/1‑138 (MQ=255)                                                                                  gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                              <  1:287285/140‑1 (MQ=255)                                                                                        gAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTtata                                        <  1:755934/140‑1 (MQ=255)                                                                                          aaCTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                <  2:414324/130‑1 (MQ=255)                                                                                          aaCTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                >  1:414324/1‑130 (MQ=255)                                                                                          aaCTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATg                                      <  1:383889/140‑1 (MQ=255)                                                                                           aCTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGc                                     <  1:13975/140‑1 (MQ=255)                                                                                                  ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                              <  2:544009/140‑1 (MQ=255)                                                                                                  ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTCACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                              <  2:637730/140‑1 (MQ=255)                                                                                                       gATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAg                                   <  2:717884/130‑1 (MQ=255)                                                                                                       gATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAg                                   >  1:717884/1‑130 (MQ=255)                                                                                                        aTTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATccc                        >  1:584341/1‑140 (MQ=255)                                                                                                                  gcgGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAggg                    >  1:919106/1‑134 (MQ=255)                                                                                                                  gcgGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAggg                    <  2:919106/134‑1 (MQ=255)                                                                                                                   cgGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATc             >  2:772405/1‑140 (MQ=255)                                                                                                                          cTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACt      >  1:414232/1‑140 (MQ=255)                                                                                                                          cTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACt      >  1:367334/1‑140 (MQ=255)                                                                                                                              aCGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCggg  >  1:388278/1‑140 (MQ=255)                                                                                                                                 gcggcgCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc       <  2:556924/132‑1 (MQ=255)                                                                                                                                 gcggcgCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc       >  1:556924/1‑132 (MQ=255)                                                                                                                                  cggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCggg  >  1:165421/1‑136 (MQ=255)                                                                                                                                  cggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCggg  <  2:165421/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    GCTCGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGG  >  NC_000913/4445416‑4445680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑