Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,487,894	G→A	A193T (GCT→ACT)	lptG →	lipopolysaccharide transport system protein LptG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,487,894	0	G	A	100.0%	151.2 / NA	43	A193T (GCT→ACT)	lptG	lipopolysaccharide transport system protein LptG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/16);  total (27/16)

AGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCGCTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACA  >  NC_000913/4487763‑4488029                                                                                                                                    |                                                                                                                                        aGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCg                                                                                                                                 >  2:547913/1‑140 (MQ=255) aGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCg                                                                                                                                 >  2:255723/1‑140 (MQ=255)   cAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGaa                                                                                                                               >  2:861345/1‑140 (MQ=255)   cAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGaa                                                                                                                               >  1:517193/1‑140 (MQ=255)            tGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGAccc                                                                                                                      <  2:900645/140‑1 (MQ=255)            tGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGAccc                                                                                                                      <  2:714372/140‑1 (MQ=255)            tGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGAccc                                                                                                                      <  2:351641/140‑1 (MQ=255)                        ggCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGt                                                                                                          <  2:517193/140‑1 (MQ=255)                             caacTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGc                                                                                                     <  1:547913/140‑1 (MQ=255)                               acTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGt                                                                                                   <  1:232178/140‑1 (MQ=255)                                  tCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTg                                                                                                >  2:858817/1‑140 (MQ=255)                                         cATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAgg                                                                                         >  1:516686/1‑140 (MQ=255)                                         cATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAgg                                                                                         >  2:296547/1‑140 (MQ=255)                                             gAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTtga                                                                                     <  1:508825/140‑1 (MQ=255)                                              aGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTtgat                                                                                    >  1:868224/1‑140 (MQ=255)                                              aGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGACGTCTGTCGCAGGTtga                                                                                     >  1:201656/1‑139 (MQ=255)                                              aGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGACGTCTGTCGCAGGTtga                                                                                     <  2:201656/139‑1 (MQ=255)                                                   gTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAGCATAAAGTCTGGCGTCTGTCGCa                                                                                           >  2:631257/1‑128 (MQ=255)                                                   gTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCa                                                                                           <  1:631257/128‑1 (MQ=255)                                                   gTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAatc                                                                               >  2:394602/1‑140 (MQ=255)                                                    ttAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAatct                                                                              >  1:544539/1‑140 (MQ=255)                                                           tGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAAtctgatctga                                                                       <  1:861345/140‑1 (MQ=255)                                                             aCGGAGAGGTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAACGTCTTGCGTCTGTCGCAGGTTGATGAATCTGATCTGAcc                                                                     >  2:648033/1‑140 (MQ=255)                                                             aCGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGAcc                                                                     >  2:327703/1‑140 (MQ=255)                                                             aCGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGAcc                                                                     >  1:143601/1‑140 (MQ=255)                                                             aCGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGAcc                                                                     >  2:752072/1‑140 (MQ=255)                                                             aCGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGAcc                                                                     >  1:734444/1‑140 (MQ=255)                                                               gAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCaa                                                                   <  2:734444/140‑1 (MQ=255)                                                                     ttAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCGGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCAGATCTGACCAATCCGaa                                                             >  2:817028/1‑140 (MQ=255)                                                                             cATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTa                                                     >  1:654361/1‑140 (MQ=255)                                                                                  gCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGt                                                >  1:458101/1‑140 (MQ=255)                                                                                        aTGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAg                                          >  1:789076/1‑140 (MQ=255)                                                                                         tGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTAACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGa                                         <  1:457796/140‑1 (MQ=255)                                                                                             tttAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGt                                     >  2:642676/1‑140 (MQ=255)                                                                                             tttAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCATCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGt                                     >  2:494927/1‑140 (MQ=255)                                                                                                    agaATCGTCGTCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGc                              <  1:736911/140‑1 (MQ=255)                                                                                                         cgtcgtCTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTg                         >  2:108128/1‑140 (MQ=255)                                                                                                           tcgtcTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGa                       >  1:560720/1‑140 (MQ=255)                                                                                                           tcgtcTGCAATCCGTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGa                       >  2:635051/1‑140 (MQ=255)                                                                                                                       cGTGCGTTATGCCTCTTCTGCGAAGTTTGACCCGGATCATAAAGTCTGGCGCCTGTCGCAGGTTGATGAATCCGAGCTGGCCAACCCGAACCAGATGACCGGTTCGCAGACGGTGAGCGGCTCCTGGAAAACCAACcaca           <  2:516686/140‑1 (MQ=255)                                                                                                                        gTACGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCAc          <  1:108128/140‑1 (MQ=255)                                                                                                                          aCGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGc        <  2:789076/140‑1 (MQ=255)                                                                                                                          aCGCTATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGc        >  2:244220/1‑140 (MQ=255)                                                                                                                              tATGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGa    <  2:458101/140‑1 (MQ=255)                                                                                                                                tGCCACTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACa  >  2:785072/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        AGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGCGGGTTAAAGGTGACGAAGAGTTAGGTGGCATCAGCATTTATGCCTTTAACGAGAATCGTCGTCTGCAATCCGTACGCTATGCCGCTACTGCGAAGTTTGACCCGGAACATAAAGTCTGGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACA  >  NC_000913/4487763‑4488029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑