Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,514,021	G→A	A96A (GCC→GCT)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,514,021	0	G	A	100.0%	77.8 / NA	23	A96A (GCC→GCT)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

GCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACG  >  NC_000913/4513908‑4514150                                                                                                                  |                                                                                                                                  gCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTgcgc                                                                                                         >  1:305610/1‑140 (MQ=255)  cGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTgcgcg                                                                                                        <  1:72667/140‑1 (MQ=255)    tAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAAGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTgcgcgcg                                                                                                      <  2:623416/140‑1 (MQ=255)      ggTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGcgcgcgcg                                                                                                    <  1:43350/140‑1 (MQ=255)                cGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGa                                                                                          >  1:608359/1‑140 (MQ=255)                 gggACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGAc                                                                                         >  2:521664/1‑140 (MQ=255)                     cTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGcc                                                                                     <  1:889188/140‑1 (MQ=255)                     cTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGcc                                                                                     <  1:521664/140‑1 (MQ=255)                              cagTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCa                                                                            >  1:335244/1‑140 (MQ=255)                               agTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAg                                                                           <  1:832526/140‑1 (MQ=255)                                      gcgcGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGcgca                                                                    <  1:191575/140‑1 (MQ=255)                                      gcgcGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTAGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGcgca                                                                    >  2:807224/1‑140 (MQ=255)                                       cgcgATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGcgcac                                                                   <  1:97038/140‑1 (MQ=255)                                         cgATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAg                                                                           >  1:437970/1‑130 (MQ=255)                                         cgATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAg                                                                           <  2:437970/130‑1 (MQ=255)                                           aTTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCgcacgcac                                                               >  1:785848/1‑140 (MQ=255)                                                 tgcAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCggg                                                         >  1:10135/1‑140 (MQ=255)                                                                    ccGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCg                                      <  2:305610/140‑1 (MQ=255)                                                                            cGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTAtcgtc                              >  1:560769/1‑140 (MQ=255)                                                                                     tCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATAcc                     <  2:335244/140‑1 (MQ=255)                                                                                     tCGGCAATGATCAGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATAcc                     >  1:205200/1‑140 (MQ=255)                                                                                                 aGGTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGAc         >  1:23697/1‑140 (MQ=255)                                                                                                   gTCTGGTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGt       <  2:827825/140‑1 (MQ=255)                                                                                                        gTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCTCGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCAcg  <  2:205200/140‑1 (MQ=255)                                                                                                        gTTTCAGAGCAGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCAcg  >  1:234462/1‑140 (MQ=255)                                                                                                                  |                                                                                                                                  GCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACG  >  NC_000913/4513908‑4514150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑