Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,610,149	T→C	F246L (TTC→CTC)	prfC →	peptide chain release factor RF3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,610,149	0	T	C	100.0%	204.4 / NA	57	F246L (TTC→CTC)	prfC	peptide chain release factor RF3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/30);  total (27/30)

TTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGTTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACTGATACCCGTACCGTAGAAG  >  NC_000913/4610015‑4610284                                                                                                                                       |                                                                                                                                        ttCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCtt                                                                                                                                    >  2:744264/1‑140 (MQ=255) ttCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCtt                                                                                                                                    >  1:565601/1‑140 (MQ=255)  tCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTg                                                                                                                                   >  2:257555/1‑140 (MQ=255)  tCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTg                                                                                                                                   >  2:130499/1‑140 (MQ=255)      gAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCggg                                                                                                                               >  2:27564/1‑140 (MQ=255)          tCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGaa                                                                                                                           >  2:842790/1‑140 (MQ=255)          tCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGaa                                                                                                                           >  2:731984/1‑140 (MQ=255)            cGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAAt                                                                                                                         <  2:855333/140‑1 (MQ=255)            cGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAAt                                                                                                                         <  1:670436/140‑1 (MQ=255)              cATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCa                                                                                                                       <  1:838353/140‑1 (MQ=255)                 tGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTc                                                                                                                    <  1:159900/140‑1 (MQ=255)                     aaaGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGaa                                                                                                                           <  2:784904/129‑1 (MQ=255)                     aaaGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGaa                                                                                                                           >  1:784904/1‑129 (MQ=255)                      aaGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGTGAAATCACTCCGGTa                                                                                                               <  1:679407/140‑1 (MQ=255)                      aaGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTa                                                                                                               <  2:565601/140‑1 (MQ=255)                         ggCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTAttc                                                                                                            >  1:734920/1‑140 (MQ=255)                           cTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGTCAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTAttctt                                                                                                          >  1:101382/1‑140 (MQ=255)                           cTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTAttctt                                                                                                          <  1:54918/140‑1 (MQ=255)                           cTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTAttctt                                                                                                          >  2:54918/1‑140 (MQ=255)                             gAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCg                                                                                                        >  2:128181/1‑140 (MQ=255)                                tAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTa                                                                                                     <  1:244166/140‑1 (MQ=255)                                    ccGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTgc                                                                                                 <  2:734920/140‑1 (MQ=255)                                     cGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAATGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTgcg                                                                                                <  1:257555/140‑1 (MQ=255)                                     cGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGTCAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTgcg                                                                                                <  2:101382/140‑1 (MQ=255)                                     cGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTgcg                                                                                                <  1:872416/140‑1 (MQ=255)                                        aTCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTa                                                                                                               >  1:161700/1‑122 (MQ=255)                                        aTCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTa                                                                                                               <  2:161700/122‑1 (MQ=255)                                        aTCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTg                                                                                             >  1:579307/1‑140 (MQ=255)                                        aTCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTg                                                                                             >  2:562094/1‑140 (MQ=255)                                           tcGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGt                                                                                          <  1:390381/140‑1 (MQ=255)                                             gATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTaa                                                                                        >  1:422401/1‑140 (MQ=255)                                               tgctgcGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACt                                                                                      >  2:613186/1‑140 (MQ=255)                                                  tgcGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCg                                                                                   <  1:562094/140‑1 (MQ=255)                                                  tgcGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCg                                                                                   >  1:265082/1‑140 (MQ=255)                                                      gTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGt                                                                               <  1:130499/140‑1 (MQ=255)                                                      gTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGt                                                                               <  2:102420/140‑1 (MQ=255)                                                               gATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCatat                                                                      <  1:430379/140‑1 (MQ=255)                                                                    ggCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTgg                                                                 >  2:100833/1‑140 (MQ=255)                                                                     gCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGa                                                                <  1:128181/140‑1 (MQ=255)                                                                     gCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGa                                                                <  1:100833/140‑1 (MQ=255)                                                                           cagcTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTT‑GATGGCCtg                                                         <  1:731984/140‑1 (MQ=255)                                                                            agcTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCtg                                                         <  1:842790/140‑1 (MQ=255)                                                                               tGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCtggtg                                                      >  2:672284/1‑140 (MQ=255)                                                                                       gaactggaactgGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGc                                              >  2:494534/1‑140 (MQ=255)                                                                                          ctggaactggTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCAcc                                           <  1:744264/140‑1 (MQ=255)                                                                                                   gTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGAt                                  >  1:56514/1‑140 (MQ=255)                                                                                                            gcgTCTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCa                         <  2:422401/140‑1 (MQ=255)                                                                                                                cTAACGAGTTCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACt                     <  2:579307/140‑1 (MQ=255)                                                                                                                         tCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCcg                              <  1:463909/122‑1 (MQ=255)                                                                                                                         tCGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCcg                              >  2:463909/1‑122 (MQ=255)                                                                                                                          cGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACTGATACccgta           >  1:546050/1‑140 (MQ=255)                                                                                                                          cGACAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACTGATACccgta           <  1:672284/140‑1 (MQ=255)                                                                                                                             cAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCa                         >  2:121696/1‑123 (MQ=255)                                                                                                                             cAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCa                         <  1:121696/123‑1 (MQ=255)                                                                                                                             cAAAGAGCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACTGATACccgtaccg        >  1:778860/1‑140 (MQ=255)                                                                                                                                 gagCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACt                     >  1:512875/1‑123 (MQ=255)                                                                                                                                 gagCTGCTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACt                     <  2:512875/123‑1 (MQ=255)                                                                                                                                   gctgctCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACTGATACCCGTACCGTAGAag  >  2:172052/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TTCAGGAAGTCCGCATTGTTAAAGGGCTGAATAACCCGGATCTCGATGCTGCGGTTGGTGAAGATCTGGCACAGCAGCTGCGTGACGAACTGGAACTGGTGAAAGGCGCGTCTAACGAGTTCGACAAAGAGCTGTTCCTTGCGGGCGAAATCACTCCGGTATTCTTCGGTACTGCGCTGGGTAACTTCGGCGTCGATCATATGTTGGATGGCCTGGTGGAGTGGGCACCTGCGCCGATGCCGCGTCAGACTGATACCCGTACCGTAGAAG  >  NC_000913/4610015‑4610284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑