Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	455,208	A→G	T26A (ACC→GCC)	tig →	trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	455,208	0	A	G	100.0%	132.5 / NA	37	T26A (ACC→GCC)	tig	trigger factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

CCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGA  >  NC_000913/455071‑455338                                                                                                                                          |                                                                                                                                   ccAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGcc                                                                                                                                  >  1:345847/1‑140 (MQ=255)  caacaaCCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGTTGACAGCATCGAGGCCg                                                                                                                                 <  2:278370/140‑1 (MQ=255)           gtgCTTGCGGGGTAAGAGTTGACCGAGCTCTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTaaaa                                                                                                                        <  1:694787/140‑1 (MQ=255)              cTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCg                                                                                                                     <  1:211268/140‑1 (MQ=255)                tGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAg                                                                                                                   <  2:52658/140‑1 (MQ=255)                tGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAg                                                                                                                   <  2:614275/140‑1 (MQ=255)                   ggggTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTg                                                                                                                >  1:340836/1‑140 (MQ=255)                      gTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTc                                                                                                             >  2:413174/1‑140 (MQ=255)                      gTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTc                                                                                                             >  1:765349/1‑140 (MQ=255)                        aaGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCaa                                                                                                           <  2:545685/140‑1 (MQ=255)                           agTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGt                                                                                                        <  2:634180/140‑1 (MQ=255)                               gACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCg                                                                                                    >  2:781491/1‑140 (MQ=255)                               gACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCACCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCg                                                                                                    >  1:403675/1‑140 (MQ=255)                                     gCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGaaaaaa                                                                                              >  2:488919/1‑140 (MQ=255)                                      cACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAg                                                                                             <  2:765349/140‑1 (MQ=255)                                         tgtgATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTAc                                                                                          <  1:413174/140‑1 (MQ=255)                                                       gTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTc                                                                            >  2:270015/1‑140 (MQ=255)                                                           cAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCgca                                                                        <  1:113382/140‑1 (MQ=255)                                                            aaGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCgcaa                                                                       <  1:270015/140‑1 (MQ=255)                                                               aTGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCgcaaagg                                                                    <  1:781491/140‑1 (MQ=255)                                                                tGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCgcaaaggc                                                                   >  1:752557/1‑140 (MQ=255)                                                                 gCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCgcaaaggca                                                                  >  1:408690/1‑140 (MQ=255)                                                                 gCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCgcaaaggca                                                                  <  2:408690/140‑1 (MQ=255)                                                                                 aCCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTg                                                             <  2:266773/129‑1 (MQ=255)                                                                                 aCCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTg                                                             >  1:266773/1‑129 (MQ=255)                                                                                        aaGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCt                                           <  2:278462/140‑1 (MQ=255)                                                                                                ggCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTa                                   >  2:456942/1‑140 (MQ=255)                                                                                                ggCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTa                                   >  1:674588/1‑140 (MQ=255)                                                                                                      cgTGTAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATGTCGTTGCTCAGCGTTATGgcgc                             >  2:345037/1‑140 (MQ=255)                                                                                                        tgtAACGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGt                           <  2:497976/140‑1 (MQ=255)                                                                                                           aaCGATTACTATCGCTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTg                        <  1:816610/140‑1 (MQ=255)                                                                                                                   cTATCACTGCTGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAg                <  2:812571/140‑1 (MQ=255)                                                                                                                        gctgctGACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGt           <  2:752557/140‑1 (MQ=255)                                                                                                                           gctgACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATGTCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCt        <  1:345037/140‑1 (MQ=255)                                                                                                                             tgACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTgg      <  1:369088/140‑1 (MQ=255)                                                                                                                              gACAGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTggg     <  1:488919/140‑1 (MQ=255)                                                                                                                                 aGCATCGAGGCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGa  >  1:730043/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   CCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGA  >  NC_000913/455071‑455338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑