Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 486,482 C→T intergenic (+74/‑54) acrR → / → mscK DNA‑binding transcriptional repressor AcrR/potassium dependent, small conductance mechanosensitive channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913486,4820CT96.2% 179.0 / ‑0.5 52intergenic (+74/‑54)acrR/mscKDNA‑binding transcriptional repressor AcrR/potassium dependent, small conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/24);  minor base . (1/1);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGC  >  NC_000913/486347‑486616
                                                                                                                                       |                                                                                                                                      
ccATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGc                                                                                                                                    >  2:850206/1‑140 (MQ=255)
              aTGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTc                                                                                                                      >  1:761599/1‑140 (MQ=255)
                gTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAg                                                                                                                    >  1:706212/1‑140 (MQ=255)
                   tctcCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTc                                                                                                                 >  2:826775/1‑140 (MQ=255)
                   tctcCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTc                                                                                                                 >  1:874347/1‑140 (MQ=255)
                        tgtgCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATT‑TTGCGGTTTGACTTTTTCAGGTCGttctt                                                                                                           >  1:667141/1‑140 (MQ=255)
                          tgCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGttcttc                                                                                                          >  1:141429/1‑140 (MQ=255)
                          tgCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGttcttc                                                                                                          >  1:103592/1‑140 (MQ=255)
                          tgCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGttcttc                                                                                                          <  1:236604/140‑1 (MQ=255)
                           cccccccGCTTCTTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCa                                                                                                         <  2:103592/139‑1 (MQ=255)
                                 cGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCa                                                                                                   >  2:873668/1‑140 (MQ=255)
                                   cTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGa                                                                                                 <  2:841750/140‑1 (MQ=255)
                                      cGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAAc                                                                                              >  2:97094/1‑140 (MQ=255)
                                            ccTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCttcat                                                                                        <  2:141429/140‑1 (MQ=255)
                                                ccACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTtcattcat                                                                                    >  1:221653/1‑140 (MQ=255)
                                                ccACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTtcattcat                                                                                    <  2:706212/140‑1 (MQ=255)
                                                   cTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTcatcat                                                                                 <  2:874347/140‑1 (MQ=255)
                                                     aaCGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGa                                                                               <  1:397965/140‑1 (MQ=255)
                                                     aaCGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGa                                                                               <  2:679774/140‑1 (MQ=255)
                                                      aCGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGAc                                                                              >  1:304896/1‑140 (MQ=255)
                                                      aCGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGAc                                                                              <  1:873668/140‑1 (MQ=255)
                                                       cGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACt                                                                             <  1:624012/140‑1 (MQ=255)
                                                          aTAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATg                                                                          <  1:826775/140‑1 (MQ=255)
                                                           tAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGt                                                                         <  2:857761/140‑1 (MQ=255)
                                                           tAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGt                                                                         >  1:342851/1‑140 (MQ=255)
                                                            aaCCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACt                                                                             <  1:511917/135‑1 (MQ=255)
                                                            aaCCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACt                                                                             >  2:511917/1‑135 (MQ=255)
                                                            aaCCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGtt                                                                        <  1:443830/140‑1 (MQ=255)
                                                               ccTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCa                                                                     <  1:198593/140‑1 (MQ=255)
                                                                cTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAg                                                                    <  2:221653/140‑1 (MQ=255)
                                                                cTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAg                                                                    >  2:160509/1‑140 (MQ=255)
                                                                 tGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGt                                                                   <  2:304896/140‑1 (MQ=255)
                                                                   aaTCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTAt                                                                 >  1:284483/1‑140 (MQ=255)
                                                                   aaTCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTAt                                                                 >  2:806737/1‑140 (MQ=255)
                                                                        gACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTcc                                                                      >  2:156659/1‑130 (MQ=255)
                                                                        gACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTcc                                                                      <  1:156659/130‑1 (MQ=255)
                                                                        gACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTa                                                                  <  1:68672/134‑1 (MQ=255)
                                                                        gACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTa                                                                  >  2:68672/1‑134 (MQ=255)
                                                                         aCTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCGGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACaaa                                                           >  1:77996/1‑140 (MQ=255)
                                                                             cAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGTGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGAt                                                       >  2:166281/1‑140 (MQ=255)
                                                                               ggATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATT‑TTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCAc                                                    <  2:667141/140‑1 (MQ=255)
                                                                                     ttCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCAt                                               >  1:354485/1‑140 (MQ=255)
                                                                                         tGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTg                                           <  1:806737/140‑1 (MQ=255)
                                                                                               ttttCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGtttttt                                     <  1:850206/140‑1 (MQ=255)
                                                                                                tttCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTc                                    <  1:343229/140‑1 (MQ=255)
                                                                                                              ttCTGGTTCACTGTGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCtt                      <  1:166281/140‑1 (MQ=255)
                                                                                                                   gTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCttttgtt                 >  2:694411/1‑140 (MQ=255)
                                                                                                                   gTTCACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCttttgtt                 >  2:550342/1‑140 (MQ=255)
                                                                                                                      cACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCttttgttttt              >  2:270980/1‑140 (MQ=255)
                                                                                                                      cACTGCGTCGTGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCttttgttttt              >  2:24355/1‑140 (MQ=255)
                                                                                                                             tcgtGATATTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGt       >  2:847154/1‑140 (MQ=255)
                                                                                                                                  atatTTTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGc  <  2:354485/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CCATCTTACTGGAGATGTATCTCCTGTGCCCCACGCTTCGTAATCCTGCCACTAACGAATAACCCTGAATCTGACTCCAGGATTTTTCCTGGACATTTTCGTCGTTGCTATTCTGGTTCACTGCGTCGTGATATTCTTGCGGTTTGACTTTTTCAGGTCGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGC  >  NC_000913/486347‑486616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑