Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	86,112	A→G	L161L (TTA→TTG)	ilvI →	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	86,112	0	A	G	100.0%	173.5 / NA	48	L161L (TTA→TTG)	ilvI	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/27);  total (21/27)

GCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAA  >  NC_000913/85976‑86248                                                                                                                                         |                                                                                                                                         gCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCg                                                                                                                                       <  2:597132/140‑1 (MQ=255)  cGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGa                                                                                                                                      <  2:843802/140‑1 (MQ=255)     cATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAg                                                                                                                                   <  1:145502/140‑1 (MQ=255)       tggtggGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGat                                                                                                                                 <  1:720740/140‑1 (MQ=255)          tggGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGcc                                                                                                                                        >  1:399856/1‑130 (MQ=255)                tttCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAAt                                                                                                                        <  1:226537/140‑1 (MQ=255)                    gcgACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCgg                                                                                                                    >  1:230515/1‑140 (MQ=255)                      gACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCg                                                                                                                  <  1:679974/140‑1 (MQ=255)                            tggtTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACaaa                                                                                                            >  2:321972/1‑140 (MQ=255)                            tggtTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTAAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACaaa                                                                                                            >  2:272124/1‑140 (MQ=255)                             ggtTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTCGCCGAAAGATATTCTTAATCCGGCGAACAAAt                                                                                                           <  1:293035/140‑1 (MQ=255)                                  aaCACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTAccc                                                                                                      >  2:686920/1‑140 (MQ=255)                                      caGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACaaa                                                                                                            <  2:383121/130‑1 (MQ=255)                                      caGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACaaa                                                                                                            >  1:383121/1‑130 (MQ=255)                                      caGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATg                                                                                                  >  1:409385/1‑140 (MQ=255)                                       aGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGt                                                                                                 <  2:667149/140‑1 (MQ=255)                                       aGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGt                                                                                                 <  1:321972/140‑1 (MQ=255)                                        gTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTc                                                                                                >  2:605052/1‑140 (MQ=255)                                            tCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGc                                                                                            <  2:393120/140‑1 (MQ=255)                                                 ttAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAg                                                                                       >  1:33990/1‑140 (MQ=255)                                                  tAAGCAAACGGAAGACATTCCGCAGGTGCTAAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGt                                                                                      <  1:272124/140‑1 (MQ=255)                                                   aaGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTc                                                                                     >  1:190499/1‑140 (MQ=255)                                                      cAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGt                                                                                  <  2:437605/140‑1 (MQ=255)                                                       aaaCGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCCCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTc                                                                                 <  2:552807/140‑1 (MQ=255)                                                           ggAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTa                                                                             <  1:686920/140‑1 (MQ=255)                                                           ggAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTa                                                                             <  2:806444/140‑1 (MQ=255)                                                              aGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGc                                                                          >  2:641324/1‑140 (MQ=255)                                                               gACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCg                                                                         <  2:190499/140‑1 (MQ=255)                                                               gACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCg                                                                         <  2:230515/140‑1 (MQ=255)                                                               gACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCg                                                                         <  1:286124/140‑1 (MQ=255)                                                                   ttCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCt                                                                     >  2:396674/1‑140 (MQ=255)                                                                       gCAGGTGCTGAAAAAGGCTTTCTGGCCGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACa                                                                 <  1:641324/140‑1 (MQ=255)                                                                        cAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACaa                                                                >  1:170641/1‑140 (MQ=255)                                                                         aGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCGGGCGAACAAATTACCCTGTGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAAt                                                               <  1:169640/140‑1 (MQ=255)                                                                         aGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAAt                                                               <  1:49426/140‑1 (MQ=255)                                                                           gTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATcc                                                             <  1:396674/140‑1 (MQ=255)                                                                              cTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCac                                                          >  2:323901/1‑140 (MQ=255)                                                                                            ctggctggCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATa                                            <  1:323901/140‑1 (MQ=255)                                                                                             tggctggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATaa                                           <  2:170641/140‑1 (MQ=255)                                                                                                 tggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAggg                                       >  1:13864/1‑140 (MQ=255)                                                                                                 tggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAggg                                       >  1:406762/1‑140 (MQ=255)                                                                                                      gcAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAAt                                  <  2:898031/140‑1 (MQ=255)                                                                                                             gTCGCCCAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGt                           >  1:855328/1‑140 (MQ=255)                                                                                                                   cAGGACCAGTAGTCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTg                     <  2:13864/140‑1 (MQ=255)                                                                                                                            tagtCGTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTg            >  2:81370/1‑140 (MQ=255)                                                                                                                                 gTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGc       >  2:243238/1‑140 (MQ=255)                                                                                                                                 gTTGATTTGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGc       >  1:723403/1‑140 (MQ=255)                                                                                                                                      tttGCCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCaa  >  2:683861/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGCAAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAA  >  NC_000913/85976‑86248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑