Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	663,851	A→G	I38T (ATC→ACC)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase DacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	663,851	0	A	G	100.0%	177.5 / NA	49	I38T (ATC→ACC)	dacA	D‑alanyl‑D‑alanine carboxypeptidase DacA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

GATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGAT  >  NC_000913/663715‑663987                                                                                                                                         |                                                                                                                                         gATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCa                                                                                                                                       <  1:330321/140‑1 (MQ=255)    aaCGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCGTCGATCTGCGGTACACCCGGGGTCATAg                                                                                                                                    <  1:70694/140‑1 (MQ=255)                catcatTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCa                                                                                                                        >  2:487435/1‑140 (MQ=255)                catcatTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCa                                                                                                                        >  1:563680/1‑140 (MQ=255)                        tGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCg                                                                                                                >  2:398338/1‑140 (MQ=255)                          gTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGc                                                                                                              <  2:563680/140‑1 (MQ=255)                              ggCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCAtgt                                                                                                          >  1:79852/1‑140 (MQ=255)                               gCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCAtgtg                                                                                                         <  1:805549/140‑1 (MQ=255)                                    cAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTgcagca                                                                                                    <  1:293747/140‑1 (MQ=255)                                    cAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTgcagca                                                                                                    <  2:162724/140‑1 (MQ=255)                                    cAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTgcagca                                                                                                    <  2:79852/140‑1 (MQ=255)                                    cAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTgcagca                                                                                                    <  1:831796/140‑1 (MQ=255)                                    cAGGACCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTgcagca                                                                                                    <  2:812002/140‑1 (MQ=255)                                         tCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTgcagca                                                                                                    <  2:427068/135‑1 (MQ=255)                                         tCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTgcagca                                                                                                    >  1:427068/1‑135 (MQ=255)                                           gcggcgGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATaa                                                                                             <  1:735931/140‑1 (MQ=255)                                                     tCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCa                                                                                   <  1:398338/140‑1 (MQ=255)                                                              tGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCa                                                                                   >  2:54099/1‑131 (MQ=255)                                                              tGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCa                                                                                   <  1:54099/131‑1 (MQ=255)                                                              tGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCgt                                                                          >  1:332235/1‑140 (MQ=255)                                                                ttCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCgtgg                                                                        >  1:595581/1‑140 (MQ=255)                                                                ttCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCgtgg                                                                        >  1:531568/1‑140 (MQ=255)                                                                ttCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGCGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCCGAAGAGATAAAGGGTGTGCAAAGAGCCgtgg                                                                        >  2:175880/1‑140 (MQ=255)                                                                         cACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAgcg                                                                  >  1:703878/1‑137 (MQ=255)                                                                         cACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAgcg                                                                  <  2:603267/137‑1 (MQ=255)                                                                         cACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAgcg                                                                  <  2:703878/137‑1 (MQ=255)                                                                         cACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAgcg                                                                  >  1:603267/1‑137 (MQ=255)                                                                            tttGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGgc                                                            <  1:28668/140‑1 (MQ=255)                                                                            tttGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGgc                                                            <  2:839435/140‑1 (MQ=255)                                                                              tGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGgcgc                                                          >  1:888690/1‑140 (MQ=255)                                                                                                 cAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGaaa                                       <  2:863800/140‑1 (MQ=255)                                                                                                    gATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGaaaaaa                                    >  1:537796/1‑140 (MQ=255)                                                                                                          ggACTCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTAt                              <  1:864049/140‑1 (MQ=255)                                                                                                              tCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGaaaaa                                     >  1:381716/1‑129 (MQ=255)                                                                                                              tCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGaaaaa                                     <  2:381716/129‑1 (MQ=255)                                                                                                              tCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGa                                         >  2:62304/1‑125 (MQ=255)                                                                                                              tCCGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGa                                         <  1:62304/125‑1 (MQ=255)                                                                                                                cGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATgg                        >  2:187199/1‑140 (MQ=255)                                                                                                                cGCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATgg                        >  2:314260/1‑140 (MQ=255)                                                                                                                 gCATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGt                       <  1:314260/140‑1 (MQ=255)                                                                                                                  cATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTc                      <  2:888690/140‑1 (MQ=255)                                                                                                                  cATCGATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTc                      <  2:537796/140‑1 (MQ=255)                                                                                                                      gATCTGCGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGaa                  <  1:915148/140‑1 (MQ=255)                                                                                                                            cGGTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCCGCAGAGGTAAAGGCTGTGCAAAGAGCCGCGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAAAGGGATTCATGGTCTGAACTACgg            >  2:401127/1‑139 (MQ=255)                                                                                                                             ggTACACCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGAc           <  2:827785/140‑1 (MQ=255)                                                                                                                                 cacCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATcc       >  2:511015/1‑140 (MQ=255)                                                                                                                                 cacCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATcc       >  2:475653/1‑140 (MQ=255)                                                                                                                                 cacCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATcc       >  1:270583/1‑140 (MQ=255)                                                                                                                                 cacCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATcc       >  1:252808/1‑140 (MQ=255)                                                                                                                                  acCCGGGGTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCg      >  1:254882/1‑140 (MQ=255)                                                                                                                                      ggggTCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGAt  <  1:187199/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAGAGATAAAGGCTGTGCAAAGAGCCGTGGTGAGCGCCAGGCGCTTCATGATACGAGCGGAAAAAATGGTATTCATGGTCTGAACTACGACATCCGTGAT  >  NC_000913/663715‑663987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑