Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 778,251 T→C V171A (GTT→GCT)  tolB → Tol‑Pal system periplasmic protein TolB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913778,2510TC100.0% 173.5 / NA 48V171A (GTT→GCT) tolBTol‑Pal system periplasmic protein TolB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/27);  total (21/27)

GGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTT  >  NC_000913/778114‑778385
                                                                                                                                         |                                                                                                                                      
gggTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTc                                                                                                                                      >  1:449952/1‑140 (MQ=255)
  gtactgtactTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAg                                                                                                                                    <  1:860959/140‑1 (MQ=255)
    actgtactTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGAc                                                                                                                                  <  2:703359/140‑1 (MQ=255)
              cTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGt                                                                                                                        <  2:857740/140‑1 (MQ=255)
               tCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGGCCAACGGCGGTc                                                                                                                       <  2:449952/140‑1 (MQ=255)
               tCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTc                                                                                                                       <  2:812641/140‑1 (MQ=255)
                cAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCa                                                                                                                      >  1:35412/1‑140 (MQ=255)
                 aGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAg                                                                                                                     >  1:178520/1‑140 (MQ=255)
                 aGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAg                                                                                                                     >  1:299406/1‑140 (MQ=255)
                  gAACTCGTACAAAGTGAACGAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGt                                                                                                                    >  2:115877/1‑140 (MQ=255)
                        gtataaaGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGt                                                                                                              >  1:824462/5‑140 (MQ=255)
                         tACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTa                                                                                                             <  2:577659/140‑1 (MQ=255)
                             aaGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGaa                                                                                                         <  2:178520/140‑1 (MQ=255)
                             aaGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGaa                                                                                                         <  2:299406/140‑1 (MQ=255)
                                  aaCAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTgcg                                                                                                    <  1:116247/140‑1 (MQ=255)
                                    cAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCTGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGcgcg                                                                                                  >  2:839491/1‑140 (MQ=255)
                                     aaGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGt                                                                                                 >  2:390939/1‑140 (MQ=255)
                                      aGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTa                                                                                                <  2:35412/140‑1 (MQ=255)
                                       gCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTAt                                                                                               <  1:82136/140‑1 (MQ=255)
                                        cAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTgcg                                                                                                    <  1:82049/134‑1 (MQ=255)
                                        cAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTgcg                                                                                                    >  2:82049/1‑134 (MQ=255)
                                        cAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATc                                                                                              <  1:333702/140‑1 (MQ=255)
                                          gTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTg                                                                                            >  2:564396/1‑140 (MQ=255)
                                          gTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTg                                                                                            <  2:707797/140‑1 (MQ=255)
                                          gTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTg                                                                                            >  1:128502/1‑140 (MQ=255)
                                          gTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTg                                                                                            >  1:271294/1‑140 (MQ=255)
                                                     aTGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGt                                                                                 <  2:271294/140‑1 (MQ=255)
                                                     aTGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGt                                                                                 <  2:128502/140‑1 (MQ=255)
                                                       gCTGGTCATACCGNCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTa                                                                               >  1:784783/1‑140 (MQ=255)
                                                          ggTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACaa                                                                            <  2:815614/140‑1 (MQ=255)
                                                           gTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAAc                                                                           <  1:352283/140‑1 (MQ=255)
                                                              aTACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAg                                                                        >  1:206864/1‑140 (MQ=255)
                                                              aTACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAg                                                                        >  2:527696/1‑140 (MQ=255)
                                                                  cGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTg                                                                    <  2:15233/140‑1 (MQ=255)
                                                                      aGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTcgt                                                                <  2:206864/140‑1 (MQ=255)
                                                                         gatgaAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTcgttca                                                             <  1:564396/140‑1 (MQ=255)
                                                                                    tGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGc                                                  <  2:295615/140‑1 (MQ=255)
                                                                                         aaCTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCg                                             <  1:1489/140‑1 (MQ=255)
                                                                                                            tGCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGt                          <  2:784783/140‑1 (MQ=255)
                                                                                                             gCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTc                         >  2:350713/1‑140 (MQ=255)
                                                                                                             gCGTTCCGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTc                         >  1:324265/1‑140 (MQ=255)
                                                                                                                   cGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGa                   >  2:310030/1‑140 (MQ=255)
                                                                                                                   cGTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGa                   <  2:233029/140‑1 (MQ=255)
                                                                                                                    gTACTCGTATTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGAc                  <  1:350713/140‑1 (MQ=255)
                                                                                                                            aTTGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTaa          >  1:852526/1‑140 (MQ=255)
                                                                                                                             ttGCCTACGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTaaa         >  1:353438/1‑140 (MQ=255)
                                                                                                                                   aCGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCt   <  1:380687/140‑1 (MQ=255)
                                                                                                                                    cGTTGCTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCtt  >  2:228333/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAACTGGCTT  >  NC_000913/778114‑778385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑