Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	936,936	G→C	G1238A (GGT→GCT)	ftsK →	cell division DNA translocase FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	936,936	0	G	C	100.0%	168.5 / NA	47	G1238A (GGT→GCT)	ftsK	cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (22/25);  total (22/25)

GCTGGCGCGGAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTC  >  NC_000913/936800‑937071                                                                                                                                         |                                                                                                                                        gCTGGCGCGGAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCg                                                                                                                                      >  1:635352/1‑140 (MQ=255)    ggCGCGGAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGccc                                                                                                                                   <  2:635352/140‑1 (MQ=255)     gcgcGGAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCca                                                                                                                                  <  2:891243/140‑1 (MQ=255)     gcgcGGAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCca                                                                                                                                  <  2:477806/140‑1 (MQ=255)     gcgcGGAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCca                                                                                                                                  <  1:401741/140‑1 (MQ=255)     gcgcGGAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCca                                                                                                                                  <  2:628306/140‑1 (MQ=255)         ggAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCGTGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGt                                                                                                                              <  1:368767/140‑1 (MQ=255)               aCTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTg                                                                                                                        <  1:26819/140‑1 (MQ=255)               aCTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTg                                                                                                                        <  1:674994/140‑1 (MQ=255)                     gggTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCa                                                                                                                  <  2:32544/140‑1 (MQ=255)                      ggTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCca                                                                                                                                  >  2:801650/1‑123 (MQ=255)                      ggTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCca                                                                                                                                  <  1:801650/123‑1 (MQ=255)                                atatGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGAc                                                                                                       >  1:253954/1‑140 (MQ=255)                                atatGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGAc                                                                                                       <  1:94293/140‑1 (MQ=255)                                atatGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGAc                                                                                                       >  1:518981/1‑140 (MQ=255)                                 tatGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACa                                                                                                      >  2:63239/1‑140 (MQ=255)                                  atGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACag                                                                                                     >  1:330444/1‑140 (MQ=255)                                         aCTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACagcgaaagc                                                                                              >  1:558628/1‑140 (MQ=255)                                         aCTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACagcgaaagc                                                                                              >  2:397070/1‑140 (MQ=255)                                              gggCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAgg                                                                                         <  1:569567/140‑1 (MQ=255)                                               ggCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAggt                                                                                        <  2:94252/140‑1 (MQ=255)                                                gCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAggtg                                                                                       <  2:518981/140‑1 (MQ=255)                                                    aaCTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc                                                                                   >  1:56812/1‑140 (MQ=255)                                                    aaCTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc                                                                                   >  2:529639/1‑140 (MQ=255)                                                    aaCTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGc                                                                                   >  2:707806/1‑140 (MQ=255)                                                         cACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGggtg                                                                              >  1:567913/1‑140 (MQ=255)                                                                   gTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATgg                                                                    <  2:558628/140‑1 (MQ=255)                                                                                gTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaaga                                                           <  2:343739/136‑1 (MQ=255)                                                                                gTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTgaaga                                                           >  1:343739/1‑136 (MQ=255)                                                                                 tGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTgg                                                      <  1:95338/140‑1 (MQ=255)                                                                                    ttttGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATc                                                   >  2:698422/1‑140 (MQ=255)                                                                                         ttCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttg                                              >  1:155911/1‑140 (MQ=255)                                                                                            gcgATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTc                                           >  1:895428/1‑140 (MQ=255)                                                                                                       gTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGt                                <  2:895428/140‑1 (MQ=255)                                                                                                          cATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttgtt                                            >  2:264259/1‑125 (MQ=255)                                                                                                          cATGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCgttgtt                                            <  1:264259/125‑1 (MQ=255)                                                                                                            tGCCGTGGTGCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGt                           >  2:861243/1‑140 (MQ=255)                                                                                                            tGCCGTGGTGCAGGACTGGAAAGCGCATGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGt                           <  1:641235/140‑1 (MQ=255)                                                                                                                  ggtgCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCa                     <  1:698422/140‑1 (MQ=255)                                                                                                                  ggtgCAGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCa                     <  1:353826/140‑1 (MQ=255)                                                                                                                       aGGACTGGAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGaa                <  2:330444/140‑1 (MQ=255)                                                                                                                              gAAAGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCa         >  1:33129/1‑140 (MQ=255)                                                                                                                                aaGCGCGTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCGGGCGGTGCAGTTTGTCACTGAAAAACGCaaa       >  1:401327/1‑140 (MQ=255)                                                                                                                                   cgcgTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCaaa       >  2:240763/1‑137 (MQ=255)                                                                                                                                   cgcgTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCaaa       <  1:240763/137‑1 (MQ=255)                                                                                                                                   cgcgTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCg    >  2:348458/1‑140 (MQ=255)                                                                                                                                     cgTGCTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTc  <  1:62802/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GCTGGCGCGGAATCACTGCTGGGTATGGGGGATATGCTCTACTCTGGGCCGAACTCCACGTTGCCGGTACGTGTCCATGGTGCTTTTGTTCGCGATCAGGAAGTTCATGCCGTGGTGCAGGACTGGAAAGCGCGTGGTCGCCCACAGTATGTTGATGGCATCACCTCCGACAGCGAAAGCGAAGGTGGTGCGGGTGGTTTCGATGGCGCTGAAGAACTGGATCCGTTGTTCGATCAGGCGGTGCAGTTTGTCACTGAAAAACGCAAAGCGTC  >  NC_000913/936800‑937071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑