Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,000	A→G	E83E (GAA→GAG)	ycbJ →	putative phosphotransferase YcbJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,000	0	A	G	100.0%	151.1 / NA	43	E83E (GAA→GAG)	ycbJ	putative phosphotransferase YcbJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/17);  total (26/17)

ACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAACACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCA  >  NC_000913/971867‑972131                                                                                                                                      |                                                                                                                                    aCCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCAcccc                                                                                                                               >  2:909304/1‑140 (MQ=255)   ccAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCgg                                                                                                                             <  2:842809/140‑1 (MQ=255)       tGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGcccc                                                                                                                         >  2:408737/1‑140 (MQ=255)        gCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCg                                                                                                                        <  1:916717/140‑1 (MQ=255)              aaTGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAATCCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTcc                                                                                                                  <  1:909304/140‑1 (MQ=255)              aaTGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTcc                                                                                                                  <  1:626767/140‑1 (MQ=255)                  gCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCt                                                                                                              >  2:860762/1‑140 (MQ=255)                                     ccGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCgtggg                                                                                           >  2:103568/1‑139 (MQ=255)                                                    aaCTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCa                                                                            <  1:408737/140‑1 (MQ=255)                                                     aCTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCAc                                                                           <  1:212527/140‑1 (MQ=255)                                                              gAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGaa                                                                  >  1:119159/1‑140 (MQ=255)                                                                   ccaccaTGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCa                                                             >  2:85034/1‑140 (MQ=255)                                                                        aTGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACg                                                        >  2:343055/1‑140 (MQ=255)                                                                         tGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGc                                                                       <  2:574599/124‑1 (MQ=255)                                                                         tGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGc                                                                       >  1:574599/1‑124 (MQ=255)                                                                         tGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGc                                                       >  2:444639/1‑140 (MQ=255)                                                                             ggCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTggg                                                   <  1:343055/140‑1 (MQ=255)                                                                              gCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGa                                                  >  1:762671/1‑140 (MQ=255)                                                                                 agaagTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGaaca                                               <  2:762671/140‑1 (MQ=255)                                                                                         gACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAg                                       >  2:333366/1‑140 (MQ=255)                                                                                          aCTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGa                                      >  1:505529/1‑140 (MQ=255)                                                                                             gTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGaacaac                                             >  2:806198/1‑130 (MQ=255)                                                                                             gTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGaacaac                                             <  1:806198/130‑1 (MQ=255)                                                                                              tCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCaa                                  >  2:769469/1‑140 (MQ=255)                                                                                               ccGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCaaa                                 >  1:316982/1‑140 (MQ=255)                                                                                                        gACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAg                        <  1:444639/140‑1 (MQ=255)                                                                                                        gACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAg                        >  2:800413/1‑140 (MQ=255)                                                                                                        gACTATTTATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAg                        >  2:14360/1‑140 (MQ=255)                                                                                                             tttATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTa                   <  2:99648/140‑1 (MQ=255)                                                                                                             tttATGGTGTCATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTa                   >  1:869789/1‑140 (MQ=255)                                                                                                              ttATGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTAc                  <  1:860762/140‑1 (MQ=255)                                                                                                                aTGGTGTGATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActg                >  1:51519/1‑140 (MQ=255)                                                                                                                     gtgATGACGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctg           >  1:92651/1‑140 (MQ=255)                                                                                                                       gatgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         >  1:132541/1‑140 (MQ=255)                                                                                                                       gatgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         <  1:769469/140‑1 (MQ=255)                                                                                                                       gatgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         >  1:591041/1‑140 (MQ=255)                                                                                                                       gatgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         <  1:333366/140‑1 (MQ=255)                                                                                                                        atgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCa        >  2:681949/1‑140 (MQ=255)                                                                                                                        atgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCa        >  1:912047/1‑140 (MQ=255)                                                                                                                        atgaCGCATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCa        <  1:85034/140‑1 (MQ=255)                                                                                                                              cATGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCa  >  2:867201/1‑140 (MQ=255)                                                                                                                                tGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         <  2:766518/131‑1 (MQ=255)                                                                                                                                tGAAGAGCACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTActggcctggc         >  1:766518/1‑131 (MQ=255)                                                                                                                                      |                                                                                                                                    ACCCAATGCCGTTAATGGCAAGAAGCTTTAGTACGCCCGGAAAAGCCCGACAACTGGCATGGAAAACCACCATGCTGGCAAGAAGTGGGACTGTCCGTATGCCGACTATTTATGGTGTGATGACGCATGAAGAACACCCCGGCCCCGATGTCCTGCTACTGGAGCGGATGCGTGGTGTTTCGGTGGAGGCACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCA  >  NC_000913/971867‑972131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑