Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	983,040	A→G	intergenic (+146/‑35)	ldtD → / → ycbK	L,D‑transpeptidase LdtD/DUF882 domain‑containing protein YcbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	983,040	0	A	G	100.0%	155.8 / NA	44	intergenic (+146/‑35)	ldtD/ycbK	L,D‑transpeptidase LdtD/DUF882 domain‑containing protein YcbK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/18);  total (26/18)

AAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACC  >  NC_000913/982911‑983176                                                                                                                                  |                                                                                                                                         aaaTCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATaa                                                                                                                                >  2:403280/1‑140 (MQ=255)     cAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACAtt                                                                                                                            <  2:522331/140‑1 (MQ=255)         tGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGAcc                                                                                                                        <  1:356843/140‑1 (MQ=255)             gTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTa                                                                                                                    >  1:695671/1‑140 (MQ=255)               aataatTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGa                                                                                                                  <  1:601923/140‑1 (MQ=255)               aataatTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGa                                                                                                                  <  1:403280/140‑1 (MQ=255)               aataatTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGa                                                                                                                  <  1:297435/140‑1 (MQ=255)                      aGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGa                                                                                                             >  2:153427/1‑138 (MQ=255)                      aGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGa                                                                                                             <  1:153427/138‑1 (MQ=255)                        cATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTAt                                                                                                         >  1:396744/1‑140 (MQ=255)                        cATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTAt                                                                                                         >  1:826758/1‑140 (MQ=255)                            aTTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATg                                                                                                     >  2:682111/1‑140 (MQ=255)                                      aTTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCg                                                                                           >  2:278862/1‑140 (MQ=255)                                                 cccccGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAAtt                                                                                             <  2:539383/127‑1 (MQ=255)                                                 cccccGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAAtt                                                                                             >  1:539383/1‑127 (MQ=255)                                                 cccccGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATcgc                                                                                >  1:268524/1‑140 (MQ=255)                                                            gctgGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCt                                                                     <  2:396744/140‑1 (MQ=255)                                                            gctgGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCt                                                                     <  2:826758/140‑1 (MQ=255)                                                             ctgGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTg                                                                    >  2:903325/1‑140 (MQ=255)                                                                  gTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCt                                                               >  1:559595/1‑140 (MQ=255)                                                                     gAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTtgg                                                            >  1:904491/1‑140 (MQ=255)                                                                                aCGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCAc                                                 >  1:48221/1‑140 (MQ=255)                                                                                aCGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCAc                                                 >  1:898275/1‑140 (MQ=255)                                                                                   tCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCg                                              <  2:898275/140‑1 (MQ=255)                                                                                          tACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTgccgc                                       >  1:267551/1‑140 (MQ=255)                                                                                            cGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCa                                     <  2:559595/140‑1 (MQ=255)                                                                                               gCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATcc                                  >  2:627005/1‑140 (MQ=255)                                                                                                  gTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGc                               >  1:369350/1‑140 (MQ=255)                                                                                                           ccTCTTGTGCGCCAGAAGTGCATGTAAACTATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTg                      >  1:135379/1‑140 (MQ=255)                                                                                                             tctTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCg                    >  2:555003/1‑140 (MQ=255)                                                                                                              ctTGTGCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGt                   <  2:625118/140‑1 (MQ=255)                                                                                                                 gtgCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTg                >  2:67540/1‑140 (MQ=255)                                                                                                                  tgCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTg                                >  1:188013/1‑123 (MQ=255)                                                                                                                  tgCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTg                                <  2:188013/123‑1 (MQ=255)                                                                                                                  tgCGCCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGc               >  1:376346/1‑140 (MQ=255)                                                                                                                   gcgcCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCa              <  1:278862/140‑1 (MQ=255)                                                                                                                    cgcCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCaa             >  2:883058/1‑140 (MQ=255)                                                                                                                     gcCAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAac            <  1:67540/140‑1 (MQ=255)                                                                                                                      ccAGAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAaca           <  1:444001/140‑1 (MQ=255)                                                                                                                         gAAGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACActc        >  1:797696/1‑140 (MQ=255)                                                                                                                           aGTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACActctc      <  1:283090/140‑1 (MQ=255)                                                                                                                            gTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAac            >  2:176986/1‑133 (MQ=255)                                                                                                                            gTGCATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAac            <  1:176986/133‑1 (MQ=255)                                                                                                                               cATGTAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTAcc  >  1:542537/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                         AAATCAGTTGTCGTAATAATTAGCATGATTGGGGGCGATTCTCTGCAGCCCCCGTCACTGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACC  >  NC_000913/982911‑983176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑