Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,595	C→T	A105A (GCC→GCT)	ampE →	protein AmpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,595	0	C	T	100.0%	219.9 / NA	62	A105A (GCC→GCT)	ampE	protein AmpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/30);  total (32/30)

TGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTT  >  NC_000913/119462‑119726                                                                                                                                      |                                                                                                                                    tGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGcc                                                                                                                               <  2:460004/140‑1 (MQ=255) tGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGcc                                                                                                                               >  1:738671/1‑140 (MQ=255)      ggAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGAt                                                                                                                          >  2:438309/1‑140 (MQ=255)           aTTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGAtggctg                                                                                                                     >  1:109273/1‑140 (MQ=255)           aTTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGAtggctg                                                                                                                     <  2:893302/140‑1 (MQ=255)            ttGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGAtggctgg                                                                                                                    <  1:887300/140‑1 (MQ=255)              gTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCg                                                                                                                  <  2:727680/140‑1 (MQ=255)              gTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCg                                                                                                                  <  1:871244/140‑1 (MQ=255)                       tCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCa                                                                                                         <  2:864829/140‑1 (MQ=255)                        cccACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCAt                                                                                                        <  2:738671/140‑1 (MQ=255)                         ccACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGaa                                                                                                                <  1:870250/131‑1 (MQ=255)                         ccACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGaa                                                                                                                >  2:870250/1‑131 (MQ=255)                         ccACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATg                                                                                                       >  2:112410/1‑140 (MQ=255)                           aCGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGAt                                                                                                     <  2:114704/140‑1 (MQ=255)                                 cTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACgg                                                                                               >  2:367354/1‑140 (MQ=255)                                         gctgctGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCg                                                                                             >  2:419671/1‑134 (MQ=255)                                         gctgctGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCg                                                                                             <  1:419671/134‑1 (MQ=255)                                         gctgctGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCgg                                                                                       >  2:438010/1‑140 (MQ=255)                                         gctgctGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCgg                                                                                       >  1:753987/1‑140 (MQ=255)                                          ctgctgATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGc                                                                                      <  1:367354/140‑1 (MQ=255)                                                     tttGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGcgacg                                                                           <  1:438010/140‑1 (MQ=255)                                                        gCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAAc                                                                        <  2:109273/140‑1 (MQ=255)                                                          tgtgTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGt                                                                      <  2:90601/140‑1 (MQ=255)                                                          tgtgTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGt                                                                      <  1:231116/140‑1 (MQ=255)                                                           gtgtATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTg                                                                     >  1:261861/1‑140 (MQ=255)                                                              tATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGt                                                                  >  1:586372/1‑140 (MQ=255)                                                              tATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGt                                                                  >  2:119202/1‑140 (MQ=255)                                                              tATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGt                                                                  >  2:24733/1‑140 (MQ=255)                                                              tATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGt                                                                  >  1:29416/1‑140 (MQ=255)                                                                      cAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGcgacg                                                                           >  2:317167/1‑123 (MQ=255)                                                                      cAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGcgacg                                                                           <  1:317167/123‑1 (MQ=255)                                                                      cAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGt                                                          >  2:763875/1‑140 (MQ=255)                                                                       gggTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTg                                                         <  2:436512/139‑1 (MQ=255)                                                                        ggTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGTCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGa                                                        >  1:173352/1‑140 (MQ=255)                                                                        ggTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGa                                                        >  1:687052/1‑140 (MQ=255)                                                                              gTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCa                                                  <  1:696324/140‑1 (MQ=255)                                                                              gTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCa                                                  <  1:119202/140‑1 (MQ=255)                                                                                 cGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCaaaa                                               <  1:286924/140‑1 (MQ=255)                                                                                   tCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATg                                             >  2:557027/1‑140 (MQ=255)                                                                                   tCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATg                                             >  2:439426/1‑140 (MQ=255)                                                                                   tCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATCCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATg                                             >  2:592674/1‑140 (MQ=255)                                                                                    cTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGc                                            <  2:438706/140‑1 (MQ=255)                                                                                     ttCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCa                                           <  1:438309/140‑1 (MQ=255)                                                                                          tATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCt                                      <  1:557027/140‑1 (MQ=255)                                                                                          tATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCt                                      <  1:439426/140‑1 (MQ=255)                                                                                          tATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCt                                      <  1:580204/140‑1 (MQ=255)                                                                                               tGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGa                                 >  1:519176/1‑140 (MQ=255)                                                                                                 cTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGAtt                               >  2:382941/1‑140 (MQ=255)                                                                                                 cTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGAtt                               >  2:156993/1‑140 (MQ=255)                                                                                                     tCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCCAAATGCATTGCTGTGGCTTaagt                           >  2:184661/1‑138 (MQ=255)                                                                                                     tCTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACt                           >  1:666851/1‑140 (MQ=255)                                                                                                      cTGACAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACtt                          >  1:64090/1‑140 (MQ=255)                                                                                                          cAGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGt                      >  1:136321/1‑140 (MQ=255)                                                                                                           aGCTGCTTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGtt                     >  2:100236/1‑140 (MQ=255)                                                                                                            gctgctTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGttt                    <  2:763440/140‑1 (MQ=255)                                                                                                            gctgctTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGttt                    <  1:112410/140‑1 (MQ=255)                                                                                                              tgctTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGttttt                  >  2:229491/1‑140 (MQ=255)                                                                                                               gctTCACGTAATGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTa                 >  1:794111/1‑140 (MQ=255)                                                                                                                         aTGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCg       <  2:687052/140‑1 (MQ=255)                                                                                                                         aTGATAGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCg       <  1:229491/140‑1 (MQ=255)                                                                                                                              aGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGtt  <  2:379433/140‑1 (MQ=255)                                                                                                                              aGCCATGCTCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGtt  >  1:433722/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    TGCAGGGAGTATTGTTCAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTT  >  NC_000913/119462‑119726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑