Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,534	A→G	D67G (GAC→GGC)	opgG →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,534	0	A	G	100.0%	181.1 / NA	50	D67G (GAC→GGC)	opgG	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/28);  total (22/28)

GGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGAT  >  NC_000913/1109397‑1109667                                                                                                                                          |                                                                                                                                      ggCTTTCAGTATTGATGATGTCACAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCa                                                                                                                                     >  1:886286/1‑140 (MQ=255)  gCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCaa                                                                                                                                    >  2:289004/1‑140 (MQ=255)    tttCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAg                                                                                                                                  <  2:571394/140‑1 (MQ=255)             tgatgaTGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGa                                                                                                                         <  1:450749/140‑1 (MQ=255)              gatgatGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGaa                                                                                                                        >  1:195835/1‑140 (MQ=255)               atgatgTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAAc                                                                                                                       <  1:345760/140‑1 (MQ=255)               atgatgTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAAc                                                                                                                       <  1:439050/140‑1 (MQ=255)               atgatgTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAAc                                                                                                                       <  2:688671/140‑1 (MQ=255)                    gTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCt                                                                                                                  >  1:655648/1‑140 (MQ=255)                      cGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGa                                                                                                                <  2:700619/140‑1 (MQ=255)                      cGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGa                                                                                                                <  2:578915/140‑1 (MQ=255)                           agcaagcTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGAcc                                                                                                           <  2:334443/140‑1 (MQ=255)                           agcaagcTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGAcc                                                                                                           <  2:886286/140‑1 (MQ=255)                             caagcTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGATGAcccc                                                                                                         >  1:313929/1‑140 (MQ=255)                                      tCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACt                                                                                                <  2:857309/140‑1 (MQ=255)                                             ccGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTc                                                                                         <  2:195835/140‑1 (MQ=255)                                             ccGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTc                                                                                         <  2:533387/140‑1 (MQ=255)                                                ggAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTAc                                                                                      >  2:897860/1‑140 (MQ=255)                                                    aGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATc                                                                                  <  2:370682/140‑1 (MQ=255)                                                    aGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATc                                                                                  <  2:90926/140‑1 (MQ=255)                                                    aGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATc                                                                                  <  2:256853/140‑1 (MQ=255)                                                            agaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGATGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATg                                                                          <  2:313929/140‑1 (MQ=255)                                                                gCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATg                                                                          >  2:144008/1‑136 (MQ=255)                                                                gCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATg                                                                          <  1:144008/136‑1 (MQ=255)                                                                gCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACt                                                                      >  1:773975/1‑140 (MQ=255)                                                                 cccAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACtt                                                                     <  2:773975/140‑1 (MQ=255)                                                                    aaaaGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGa                                                                  >  2:822447/1‑140 (MQ=255)                                                                            cTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCgg                                                          >  2:181427/1‑140 (MQ=255)                                                                            cTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCgg                                                          >  1:448714/1‑140 (MQ=255)                                                                               gCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATA‑CCCGGTCaa                                                      <  1:508446/140‑1 (MQ=255)                                                                                cccTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGCACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCaa                                                      <  2:834321/140‑1 (MQ=255)                                                                                cccTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCaa                                                      <  2:673762/140‑1 (MQ=255)                                                                                    ccGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAaaata                                                  >  2:870652/1‑140 (MQ=255)                                                                                                           gcgGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                           >  1:169241/1‑140 (MQ=255)                                                                                                           gcgGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                           >  1:394246/1‑140 (MQ=255)                                                                                                           gcgGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                           >  2:80051/1‑140 (MQ=255)                                                                                                           gcgGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                           <  1:897860/140‑1 (MQ=255)                                                                                                               aCTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCaaa                       >  2:548851/1‑140 (MQ=255)                                                                                                                cTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAAc                      <  1:80051/140‑1 (MQ=255)                                                                                                                cTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAAc                      <  1:289004/140‑1 (MQ=255)                                                                                                                     agcagATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATc                 >  1:417218/1‑140 (MQ=255)                                                                                                                          aTCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATa            <  1:822447/140‑1 (MQ=255)                                                                                                                           tCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATAc           >  2:65155/1‑140 (MQ=255)                                                                                                                           tCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATAc           >  2:425985/1‑140 (MQ=255)                                                                                                                               gTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGcc       >  2:380001/1‑140 (MQ=255)                                                                                                                                 ttAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCg     >  2:405513/1‑140 (MQ=255)                                                                                                                                 ttAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCg     >  1:94598/1‑140 (MQ=255)                                                                                                                                   aaTCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGa   <  1:819134/140‑1 (MQ=255)                                                                                                                                   aaTCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGCTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACAAATCAAATACAGCCCGGa   <  2:655648/140‑1 (MQ=255)                                                                                                                                    aTCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGat  <  2:394246/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGAT  >  NC_000913/1109397‑1109667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑