Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,120,815	A→G	G47G (GGT→GGC)	bssS ←	regulator of biofilm formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,120,815	0	A	G	100.0%	207.5 / NA	59	G47G (GGT→GGC)	bssS	regulator of biofilm formation
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/28);  total (31/28)

TGGGTGGATGGTCATGTCATGTTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGACCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTCATTATTTT  >  NC_000913/1120680‑1120948                                                                                                                                        |                                                                                                                                      tGGGTGGATGGTCATGTCATGTTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTTGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGcc                                                                                                                                     >  1:583570/1‑138 (MQ=255)    gTGGATGGTCATGTCATGTTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACtt                                                                                                                                <  2:641455/140‑1 (MQ=255)    gTGGATGGTCATGTCATGTTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACtt                                                                                                                                <  2:177064/140‑1 (MQ=255)       gATGGTCATGTCATGTTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAg                                                                                                                             >  2:238728/1‑140 (MQ=255)        aTGGTCATGTCATGTTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGt                                                                                                                            >  2:542948/1‑140 (MQ=255)            tcatgtcatgTTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGCGCGTCTGGCCAACTTCAGTCCCt                                                                                                                        >  2:509501/1‑140 (MQ=255)               tgtcatgtTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTcc                                                                                                                     >  1:275043/1‑140 (MQ=255)                     gtTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACtt                                                                                                                                <  2:795082/123‑1 (MQ=255)                     gtTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACtt                                                                                                                                >  1:795082/1‑123 (MQ=255)                      ttAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTc                                                                                                                      >  1:763282/1‑132 (MQ=255)                      ttAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTc                                                                                                                      <  2:763282/132‑1 (MQ=255)                      ttAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCgg                                                                                                              >  2:709123/1‑140 (MQ=255)                       tAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGa                                                                                                             <  1:845270/140‑1 (MQ=255)                          tGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACtt                                                                                                          >  2:65477/1‑140 (MQ=255)                           gACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTa                                                                                                         >  1:388023/1‑140 (MQ=255)                             cGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTGtt                                                                                                       <  1:618706/140‑1 (MQ=255)                             cGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTAtt                                                                                                       <  1:740411/140‑1 (MQ=255)                              gCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTAttt                                                                                                      >  1:582499/1‑140 (MQ=255)                               ccGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTc                                                                                                                <  2:78570/129‑1 (MQ=255)                               ccGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTc                                                                                                                >  1:78570/1‑129 (MQ=255)                               ccGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTg                                                                                                     <  1:542948/140‑1 (MQ=255)                                  aTACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTgggg                                                                                                  >  2:511941/1‑140 (MQ=255)                                  aTACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTgggg                                                                                                  >  1:220082/1‑140 (MQ=255)                                        gTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTAtttt                                                                                                     >  2:158622/1‑130 (MQ=255)                                        gTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTAtttt                                                                                                     <  1:158622/131‑2 (MQ=255)                                         tttACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTa                                                                                           >  2:467453/1‑140 (MQ=255)                                         tttACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTa                                                                                           <  2:275043/140‑1 (MQ=255)                                           tACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTg                                                                                                     >  2:798984/1‑128 (MQ=255)                                           tACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTg                                                                                                     <  1:798984/128‑1 (MQ=255)                                              cTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCa                                                                                      <  2:220082/140‑1 (MQ=255)                                                       aCCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACa                                                                             >  1:876948/1‑140 (MQ=255)                                                           gATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAg                                                                         <  2:649065/140‑1 (MQ=255)                                                            aTTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCa                                                                                      <  2:229897/126‑1 (MQ=255)                                                            aTTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCa                                                                                      >  1:229897/1‑126 (MQ=255)                                                            aTTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAgc                                                                        <  1:525238/140‑1 (MQ=255)                                                                  ttttGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGcatca                                                                  <  1:238728/140‑1 (MQ=255)                                                                          aTTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATc                                                          <  1:709123/140‑1 (MQ=255)                                                                            tCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCaa                                                        <  1:65477/140‑1 (MQ=255)                                                                              cTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACg                                                      <  1:142678/140‑1 (MQ=255)                                                                                 cTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTg                                                   >  2:143292/1‑140 (MQ=255)                                                                                 cTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTg                                                   >  1:139608/1‑140 (MQ=255)                                                                                    cTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTg                                                <  1:656783/140‑1 (MQ=255)                                                                                    cTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTg                                                <  1:509501/140‑1 (MQ=255)                                                                                    cTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTg                                                <  2:388023/140‑1 (MQ=255)                                                                                     tAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGa                                               >  1:576813/1‑140 (MQ=255)                                                                                          tCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTcc                                          <  1:817229/140‑1 (MQ=255)                                                                                          tCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTcc                                          <  1:511941/140‑1 (MQ=255)                                                                                           cGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTccc                                         <  1:143292/140‑1 (MQ=255)                                                                                              aataaatTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCAcc                                      >  1:93314/1‑140 (MQ=255)                                                                                              aataaatTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCAcc                                      >  1:350497/1‑140 (MQ=255)                                                                                                       tCTGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCg                             >  1:133104/1‑140 (MQ=255)                                                                                                         tGGCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGa                           <  2:139608/140‑1 (MQ=255)                                                                                                           gCAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATg                         >  1:648071/1‑140 (MQ=255)                                                                                                            cAACATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGa                        >  1:910720/1‑140 (MQ=255)                                                                                                               cATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTc                     <  2:133104/140‑1 (MQ=255)                                                                                                               cATCAGTGGTTAACCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTc                     >  1:549371/1‑140 (MQ=255)                                                                                                                          aaCCAGAGCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTc          >  2:88677/1‑140 (MQ=255)                                                                                                                                 gCGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTCATTAttt   >  2:672681/1‑140 (MQ=255)                                                                                                                                  cGTCTGGCCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTCATTAtttt  >  2:634326/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TGGGTGGATGGTCATGTCATGTTAATGACGCCGATACTCGTTTACCTGGAAATCACCGGATTCAATTTTGGCGATTCCTGCTTCTAATATCGAAATAAATTGTCTGGCAACATCAGTGGTTAACCAGAGCGTCTGACCAACTTCAGTCCCTTCCTGCTCGGACTTATTTGGGGTCTGGTAGTGCAAACGCAACATCAGCGCATCATAGCTATCAACGGTGCTGATGTCCCACCCTACGAGCGGATGAGTCTGAATGACTTCATTATTTT  >  NC_000913/1120680‑1120948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑