Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,195,426	C→T	P102S (CCG→TCG)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,195,426	0	C	T	100.0%	185.4 / NA	53	P102S (CCG→TCG)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/27);  total (26/27)

GCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGAC  >  NC_000913/1195298‑1195562                                                                                                                                 |                                                                                                                                         gCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACt                                                                                                                               <  2:302883/140‑1 (MQ=255)      cGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCg                                                                                                                                        >  2:329122/1‑126 (MQ=255)      cGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCg                                                                                                                                        <  1:329122/126‑1 (MQ=255)                       ggaAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGct                                                                                                         <  2:311345/140‑1 (MQ=255)                         aaaTTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGctct                                                                                                       >  1:585660/1‑140 (MQ=255)                                     gTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGcc                                                                                           >  2:722802/1‑140 (MQ=255)                                      tGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGccc                                                                                          >  2:397078/1‑140 (MQ=255)                                      tGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGccc                                                                                          >  2:438082/1‑140 (MQ=255)                                      tGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGccc                                                                                          >  1:459841/1‑140 (MQ=255)                                               cacacaGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAgg                                                                                 >  2:151520/1‑140 (MQ=255)                                                acacaGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGa                                                                                <  2:698791/140‑1 (MQ=255)                                                acacaGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGa                                                                                <  2:870427/140‑1 (MQ=255)                                                  acaGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAAc                                                                              <  2:1568/140‑1 (MQ=255)                                                         tATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGAtct                                                                       <  2:609557/140‑1 (MQ=255)                                                              tCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACa                                                                  >  2:498215/1‑140 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTgg                                                                           <  2:624639/129‑1 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTgg                                                                           >  1:624639/1‑129 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATc                                                                >  1:358941/1‑140 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATc                                                                >  1:642274/1‑140 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATc                                                                >  1:637253/1‑140 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATc                                                                >  2:746928/1‑140 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATc                                                                >  1:340559/1‑140 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATc                                                                >  2:333007/1‑140 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCCCTGAACGTTGCCCTGCGCCAGGAACTGGAt                                                                         >  1:673573/1‑131 (MQ=255)                                                                aGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCCCTGAACGTTGCCCTGCGCCAGGAACTGGAt                                                                         <  2:673573/131‑1 (MQ=255)                                                                   aCGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATctgc                                                             <  1:381855/140‑1 (MQ=255)                                                                       cTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATctgcctgc                                                         <  1:397078/140‑1 (MQ=255)                                                                        tGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCg                                                        >  1:514150/1‑140 (MQ=255)                                                                           ctgcctgcTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTcc                                                     <  1:780869/140‑1 (MQ=255)                                                                              cctgcTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGt                                                  <  2:642274/140‑1 (MQ=255)                                                                              cctgcTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGt                                                  <  1:353852/140‑1 (MQ=255)                                                                                tgctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCg                                                    <  2:121200/136‑1 (MQ=255)                                                                                tgctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCg                                                    >  1:121200/1‑136 (MQ=255)                                                                                  ctgAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGt                                              <  1:179415/140‑1 (MQ=255)                                                                                       aCTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGt                                                  >  2:914170/1‑131 (MQ=255)                                                                                       aCTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGt                                                  <  1:914170/131‑1 (MQ=255)                                                                                        ctctTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTAt                                        <  2:585660/140‑1 (MQ=255)                                                                                        ctctTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTAt                                        >  2:736980/1‑140 (MQ=255)                                                                                        ctctTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTAt                                        >  1:445210/1‑140 (MQ=255)                                                                                           ttGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAg                                     >  1:34546/1‑140 (MQ=255)                                                                                             gATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTCCGTTACTATCAggg                                   >  1:305101/1‑140 (MQ=255)                                                                                             gATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAggg                                   <  2:304952/140‑1 (MQ=255)                                                                                             gATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAggg                                   <  2:305101/140‑1 (MQ=255)                                                                                             gATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAggg                                   <  1:897273/140‑1 (MQ=255)                                                                                             gATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAggg                                   <  1:438082/140‑1 (MQ=255)                                                                                              aTCTGATTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGc                                  <  1:333007/140‑1 (MQ=255)                                                                                                   aTTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCTGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTcc                             >  2:254408/1‑140 (MQ=255)                                                                                                   aTTCGTGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTcc                             >  1:223892/1‑140 (MQ=255)                                                                                                        tGAATATCGCGTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGcc                        <  2:340559/140‑1 (MQ=255)                                                                                                               cgcgTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTaa                 <  1:722802/140‑1 (MQ=255)                                                                                                                 cgTTGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAac               <  2:637253/140‑1 (MQ=255)                                                                                                                    tGCCATTAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACAcc            <  1:746928/140‑1 (MQ=255)                                                                                                                          tAAAGGTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAAc      >  1:347935/1‑140 (MQ=255)                                                                                                                              ggTTCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGAc  <  1:498215/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         GCGAGCGTAAAATCTCCTGGATGGAAATTTACACCGGTGAAAAATCCACACAGGTTTATGGTCAGGACGTCTGGCTGCCTGCTGAAACTCTTGATCTGATTCGTGAATATCGCGTTGCCATTAAAGGTCCGCTGACCACTCCGGTTGGTGGCGGTATTCGCTCTCTGAACGTTGCCCTGCGCCAGGAACTGGATCTCTACATCTGCCTGCGTCCGGTACGTTACTATCAGGGCACTCCAAGCCCGGTTAAACACCCTGAACTGAC  >  NC_000913/1195298‑1195562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑