Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,207,903	T→C	W135R (TGG→CGG)	ycfK →	e14 prophage; protein StfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,207,903	0	T	C	100.0%	209.2 / NA	58	W135R (TGG→CGG)	ycfK	e14 prophage; protein StfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

TGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTG  >  NC_000913/1207769‑1208037                                                                                                                                       |                                                                                                                                       tgCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCggggg                                                                                                                                   >  2:258840/1‑140 (MQ=255)     cttcattttcataaacgTTGGTTTGGGGGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGa                                                                                                                               <  1:906004‑M1/123‑1 (MQ=255)         attttcataaacgTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGc                                                                                                                           <  2:540788‑M1/127‑1 (MQ=255)           tctcGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCg                                                                                                                         >  2:189503/1‑140 (MQ=255)               ataaacgTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCaaa                                                                                                                     <  1:257521‑M1/133‑1 (MQ=255)                        GGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGc                                                                                                            <  1:687494‑M1/140‑1 (MQ=255)                        GGTTTGGGAGA‑GGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGcc                                                                                                           <  1:267378‑M1/140‑1 (MQ=255)                               GAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTggg                                                                                                     <  1:141259‑M1/140‑1 (MQ=255)                                 GAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTggggg                                                                                                   <  2:231704‑M1/140‑1 (MQ=255)                                     GAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGAt                                                                                               >  1:320798‑M1/1‑140 (MQ=255)                                         aaaaCTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGaa                                                                                           >  2:187738/1‑140 (MQ=255)                                         aaaaCTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGaa                                                                                           <  1:893103/140‑1 (MQ=255)                                                aaTGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGt                                                                                    >  2:518590/1‑140 (MQ=255)                                                      gcaACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTAtt                                                                              <  1:347001/140‑1 (MQ=255)                                                      gcaACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTAtt                                                                              <  2:687494/140‑1 (MQ=255)                                                       caACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTc                                                                             >  2:728181/1‑140 (MQ=255)                                                           ggCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCgg                                                                         <  1:187738/140‑1 (MQ=255)                                                             cTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGtt                                                                       <  1:518590/140‑1 (MQ=255)                                                                cATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCg                                                                    <  2:286311/140‑1 (MQ=255)                                                                cATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCg                                                                    <  1:777587/140‑1 (MQ=255)                                                                   tAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGttt                                                                 <  1:861174/140‑1 (MQ=255)                                                                     ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGtt                                                                       <  1:403869/132‑1 (MQ=255)                                                                     ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGtt                                                                       >  2:403869/1‑132 (MQ=255)                                                                     ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                               <  1:133715/140‑1 (MQ=255)                                                                     ggACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                               >  1:369949/1‑140 (MQ=255)                                                                      gACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaat                                                              <  1:231704/140‑1 (MQ=255)                                                                         gCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTaataat                                                           <  1:258840/140‑1 (MQ=255)                                                                          cACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATg                                                          >  1:853222/1‑140 (MQ=255)                                                                           aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                               >  1:450009/1‑134 (MQ=255)                                                                           aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                               <  2:450009/134‑1 (MQ=255)                                                                           aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGc                                                         <  1:540788/140‑1 (MQ=255)                                                                                             ccGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGc                                       >  1:755610/1‑140 (MQ=255)                                                                                                gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                                    >  2:700678/1‑140 (MQ=255)                                                                                                gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                                    >  1:411748/1‑140 (MQ=255)                                                                                                gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                                    <  2:755610/140‑1 (MQ=255)                                                                                                  tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                        >  1:282326/1‑134 (MQ=255)                                                                                                  tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                                        <  2:282326/134‑1 (MQ=255)                                                                                                  tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAAtt                                  <  2:906004/140‑1 (MQ=255)                                                                                                     gATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGTAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATg                               >  2:138140/1‑140 (MQ=255)                                                                                                     gATTGGTGGTATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATg                               >  2:192584/1‑140 (MQ=255)                                                                                                          gtggtATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAAt                          >  1:639864/1‑140 (MQ=255)                                                                                                          gtggtATTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAAt                          >  1:120444/1‑140 (MQ=255)                                                                                                               aTTGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAg                     >  2:483863/1‑140 (MQ=255)                                                                                                                ttGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGTAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgc                    <  1:138140/140‑1 (MQ=255)                                                                                                                 tGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcg                   <  2:188899/140‑1 (MQ=255)                                                                                                                 tGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcg                   <  2:444235/140‑1 (MQ=255)                                                                                                                 tGAGCAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcg                   <  2:766275/140‑1 (MQ=255)                                                                                                                     cAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc               >  2:214377/1‑140 (MQ=255)                                                                                                                     cAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc               <  2:141259/140‑1 (MQ=255)                                                                                                                     cAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc               >  1:402165/1‑140 (MQ=255)                                                                                                                     cAATCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAcc               >  1:191989/1‑140 (MQ=255)                                                                                                                        tCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                 <  1:780364/135‑1 (MQ=255)                                                                                                                        tCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCa                 >  2:780364/1‑135 (MQ=255)                                                                                                                        tCAGTAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTAt            >  2:354833/1‑140 (MQ=255)                                                                                                                            tAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAAt        >  1:169087/1‑140 (MQ=255)                                                                                                                            tAATCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAAt        >  1:124633/1‑140 (MQ=255)                                                                                                                             aaTCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGt                        <  1:446451/123‑1 (MQ=255)                                                                                                                             aaTCATTCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGt                        >  2:446451/1‑123 (MQ=255)                                                                                                                                  ttCAGCGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTg  >  2:221713/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       TGCAAACGGCTCTCGAAAACCTTGGTTTGGGAGAAGGAGCAAAACTCAATGCAGCAACGGCTACATTAGGACGCACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGGGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAGTG  >  NC_000913/1207769‑1208037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑