Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,519	A→G	N302S (AAC→AGC)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,519	0	A	G	100.0%	157.2 / NA	45	N302S (AAC→AGC)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/25);  total (20/25)

TGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATT  >  NC_000913/132384‑132649                                                                                                                                        |                                                                                                                                   tGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATg                                                                                                                                <  1:265828/140‑1 (MQ=255)      gatgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGa                                                                                                                           <  1:905909/140‑1 (MQ=255)      gatgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAAACTGAGCATGGGCGa                                                                                                                           >  1:306349/1‑140 (MQ=255)          atatTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTg                                                                                                                       <  1:145173/140‑1 (MQ=255)              tCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTg                                                                                                                   >  1:534875/1‑140 (MQ=255)               ccACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGGAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGGACTGCCAATCGAAGTCGACGTCTCTAAACTGAGCATGGGCGACGTGAttta                                                                                                                  >  2:255172/1‑138 (MQ=255)                     gtgCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGCACTGCCAAACGAAGTCGACGTCTCTAAACTGAGCATGGGCGACGGGATTGACGTTTa                                                                                                            >  2:145252/1‑140 (MQ=255)                      tgCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTAc                                                                                                           >  2:10140/1‑140 (MQ=255)                      tgCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTAc                                                                                                           <  1:145252/140‑1 (MQ=255)                       gCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTAcc                                                                                                          >  2:49246/1‑140 (MQ=255)                        ccGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTAccc                                                                                                         >  1:376352/1‑140 (MQ=255)                         cGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCg                                                                                                        <  2:376352/140‑1 (MQ=255)                           aaCAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTa                                                                                                      <  1:780840/140‑1 (MQ=255)                                aCGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGCACTGCCCATCGAAGTCGACGTCTCTAACCCGAGCATGGGCGACGTGATTGACGTTTACCCCTACaaaa                                                                                                 >  2:423442/1‑139 (MQ=255)                                    ggcggTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGa                                                                                             <  1:108482/140‑1 (MQ=255)                                           gtTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGt                                                                                      <  2:534875/140‑1 (MQ=255)                                            tttGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTa                                                                                     <  1:10140/140‑1 (MQ=255)                                                    tCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGaa                                                                             <  1:855634/140‑1 (MQ=255)                                                      ggcggTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGa                                                                              <  2:896157/137‑1 (MQ=255)                                                      ggcggTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGa                                                                              >  1:896157/1‑137 (MQ=255)                                                                     cccATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGc                                                            <  2:200493/140‑1 (MQ=255)                                                                     cccATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGc                                                            >  1:177601/1‑140 (MQ=255)                                                                     cccATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGc                                                            >  2:194506/1‑140 (MQ=255)                                                                          cttcttTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCt                                                       >  1:97398/1‑140 (MQ=255)                                                                                  acacGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTg                                               >  1:454778/1‑140 (MQ=255)                                                                                    acGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGaa                                             >  2:386044/1‑140 (MQ=255)                                                                                    acGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGaa                                             >  2:651408/1‑140 (MQ=255)                                                                                    acGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGaa                                             <  2:97398/140‑1 (MQ=255)                                                                                       aTGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAATCGGCGAACTGCTGGCGACCTTCGAACTGAAAAc                                          >  1:823831/1‑140 (MQ=255)                                                                                       aTGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAAc                                          >  1:358241/1‑140 (MQ=255)                                                                                       aTGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAAc                                          >  2:722511/1‑140 (MQ=255)                                                                                       aTGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAAc                                          >  1:372947/1‑140 (MQ=255)                                                                                        tGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAAcc                                         >  2:11600/1‑140 (MQ=255)                                                                                        tGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAAcc                                         <  1:423442/140‑1 (MQ=255)                                                                                         ggAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCg                                        <  2:853774/140‑1 (MQ=255)                                                                                              aCGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTg                                   <  2:358241/140‑1 (MQ=255)                                                                                                gcgGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCt                                 >  1:510807/1‑140 (MQ=255)                                                                                                  gggTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGt                                    <  2:363087/135‑1 (MQ=255)                                                                                                  gggTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGt                                    >  1:363087/1‑135 (MQ=255)                                                                                                  gggTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCtga                               <  1:722511/140‑1 (MQ=255)                                                                                                    gTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCtga                               <  1:97167/138‑1 (MQ=255)                                                                                                    gTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCtga                               >  2:97167/1‑138 (MQ=255)                                                                                                     tGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCtgattg                            >  2:129097/1‑140 (MQ=255)                                                                                                                  gAAGTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAgtgcgtgc               <  1:49246/140‑1 (MQ=255)                                                                                                                     gTCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCtgg            <  2:372947/140‑1 (MQ=255)                                                                                                                      tCGACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCtggt           >  1:95210/1‑140 (MQ=255)                                                                                                                        gACGTCTCTAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCtggtgg         <  1:129097/140‑1 (MQ=255)                                                                                                                               ctAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACTAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTAtt  <  2:376867/140‑1 (MQ=255)                                                                                                                               ctAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTAtt  <  2:505874/140‑1 (MQ=255)                                                                                                                               ctAACCTGAGCATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTAtt  <  1:386044/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   TGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATT  >  NC_000913/132384‑132649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑