Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,437,183	T→C	noncoding (63/109 nt)	micC →	small regulatory RNA MicC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,437,183	0	T	C	100.0%	139.5 / NA	40	noncoding (63/109 nt)	micC	small regulatory RNA MicC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/18);  total (22/18)

TCGAAGATAATACATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGC  >  NC_000913/1437058‑1437315                                                                                                                              |                                                                                                                                     tCGAAGATAATACATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATaaaaa                                                                                                                        >  2:764508/1‑140 (MQ=255)  cGAAGATAATACATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTccc                                                                                                                                    >  1:590552/1‑127 (MQ=255)  cGAAGATAATACATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAg                                                                                                                       >  2:413490/1‑140 (MQ=255)   gAAGATAATACATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGa                                                                                                                      <  2:371595/140‑1 (MQ=255)   gAAGATAATACATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGa                                                                                                                      <  2:425489/140‑1 (MQ=255)      gATAATACATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACaa                                                                                                                   <  2:300400/140‑1 (MQ=255)         aataCATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGcc                                                                                                                >  2:363204/1‑140 (MQ=255)           tacatacaAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCg                                                                                                              >  2:133540/1‑140 (MQ=255)           tacatacaAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCg                                                                                                              <  1:730810/140‑1 (MQ=255)                acaAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAg                                                                                                         >  1:882383/1‑140 (MQ=255)                 caAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAgt                                                                                                        >  1:685765/1‑140 (MQ=255)                    aaTAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAgtcgt                                                                                                     >  2:543843/1‑140 (MQ=255)                                  ttttAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCtttttttt                                                                                       <  2:882383/140‑1 (MQ=255)                                  ttttAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCtttttttt                                                                                       <  1:363204/140‑1 (MQ=255)                                    ttAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTa                                                                                     >  1:417792/1‑140 (MQ=255)                                    ttAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTa                                                                                     >  2:289835/1‑140 (MQ=255)                                    ttAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTAGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTa                                                                                     >  1:801420/1‑140 (MQ=255)                                             tataCGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGAt                                                                            <  2:417792/140‑1 (MQ=255)                                                  gTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATcc                                                                       <  1:764508/140‑1 (MQ=255)                                                      ttCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTAt                                                                   >  2:445126/1‑140 (MQ=255)                                                      ttCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTAt                                                                   >  2:194433/1‑140 (MQ=255)                                                         tGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCa                                                                <  1:644814/140‑1 (MQ=255)                                                          gcgcGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCag                                                               <  2:475899/140‑1 (MQ=255)                                                           cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCaga                                                              >  2:121707/1‑140 (MQ=255)                                                            gcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCagag                                                             >  1:109034/1‑140 (MQ=255)                                                               ggTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCAt                                                          <  1:289835/140‑1 (MQ=255)                                                                    tatGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAAt                                                     <  2:663172/140‑1 (MQ=255)                                                                            tATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTg                                             >  1:432024/1‑140 (MQ=255)                                                                             aTTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGc                                            >  1:420383/1‑140 (MQ=255)                                                                                    caGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTcc                                    >  1:387784/1‑140 (MQ=255)                                                                                           tattttCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCtttt                              <  2:801420/140‑1 (MQ=255)                                                                                           tattttCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCtttt                              <  1:445126/140‑1 (MQ=255)                                                                                           tattttCTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCtttt                              >  2:838229/1‑140 (MQ=255)                                                                                                 cTGTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTACCGCTTTTCCGGCCa                       <  2:387784/140‑1 (MQ=255)                                                                                                   gTTGGGCCATTGCATTGCCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCAt                      <  2:432024/140‑1 (MQ=255)                                                                                                            ttgcattgcCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTtgt                  >  2:156988/1‑135 (MQ=255)                                                                                                            ttgcattgcCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTtgt                  <  1:156988/135‑1 (MQ=255)                                                                                                             tgcattgcCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAgg            <  1:194433/140‑1 (MQ=255)                                                                                                                 ttgcCACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGt        >  2:64181/1‑140 (MQ=255)                                                                                                                     cACTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATa    >  2:608681/1‑140 (MQ=255)                                                                                                                       cTGATTTCCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGc  >  1:574809/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                     TCGAAGATAATACATACAAAATAAAAATTATACTTTTAATTTGCTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGC  >  NC_000913/1437058‑1437315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑