Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,463,955	A→G	A139A (GCA→GCG)	paaX →	DNA‑binding transcriptional repressor PaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,463,955	0	A	G	100.0%	126.8 / NA	37	A139A (GCA→GCG)	paaX	DNA‑binding transcriptional repressor PaaX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

GTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCA  >  NC_000913/1463825‑1464091                                                                                                                                   |                                                                                                                                         gTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTg                                                                                                                                 <  2:85785/140‑1 (MQ=255) gTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTg                                                                                                                                 <  2:146769/140‑1 (MQ=255)    aaaTTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATg                                                                                                                              >  1:430575/1‑140 (MQ=255)            cgcgCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGcc                                                                                                                      <  1:149860/140‑1 (MQ=255)                      aaCCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCaaaaa                                                                                                            <  1:317298/140‑1 (MQ=255)                        ccTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACt                                                                                                          <  2:86951/140‑1 (MQ=255)                             aTGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCg                                                                                                     >  2:878244/1‑140 (MQ=255)                                  aTGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTa                                                                                                >  1:372333/1‑140 (MQ=255)                                      tAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGa                                                                                            >  2:437403/1‑140 (MQ=255)                                          tGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCATTTGATCTGGCAAGTTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAGCTGGCCGATGTACAGACACt                                                                                        <  1:229455/140‑1 (MQ=255)                                           ggCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACtt                                                                                       <  1:5960/140‑1 (MQ=255)                                                cTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCa                                                                                  >  1:127767/1‑140 (MQ=255)                                                  gTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATg                                                                                >  2:250213/1‑140 (MQ=255)                                                   ttGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGa                                                                               >  1:254672/1‑140 (MQ=255)                                                      ctctCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGc                                                                            >  1:905816/1‑140 (MQ=255)                                                            gAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGgtgt                                                                      <  1:334112/140‑1 (MQ=255)                                                            gAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGgtgt                                                                      <  1:447318/140‑1 (MQ=255)                                                                   tAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGAt                                                               >  1:426105/1‑140 (MQ=255)                                                                           tCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGAt                                                       >  1:288423/1‑140 (MQ=255)                                                                           tCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGAt                                                       <  2:372333/140‑1 (MQ=255)                                                                                    gctgATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGa                                              >  2:612850/1‑140 (MQ=255)                                                                                     ctgATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGaa                                             <  1:250213/140‑1 (MQ=255)                                                                                      tgATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAg                                            <  2:288423/140‑1 (MQ=255)                                                                                        aTGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAgcg                                          >  2:868612/1‑140 (MQ=255)                                                                                          gTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCa                                        <  1:330379/140‑1 (MQ=255)                                                                                                  gCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCAc                                <  2:905816/140‑1 (MQ=255)                                                                                                     gTTGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTgg                             >  2:325288/1‑140 (MQ=255)                                                                                                       tGATCTGGCAAGGTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGgcg                           <  2:127767/140‑1 (MQ=255)                                                                                                                   gTTTTGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCgcgcg               >  1:146328/1‑139 (MQ=255)                                                                                                                    ttttGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCgcag              <  1:437403/140‑1 (MQ=255)                                                                                                                    ttttGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCgcag              <  1:325288/140‑1 (MQ=255)                                                                                                                     tttGGCGCACTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCgcagc             >  1:150683/1‑140 (MQ=255)                                                                                                                              cTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGc    <  2:125649/140‑1 (MQ=255)                                                                                                                              cTGGCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGc    >  1:125649/1‑140 (MQ=255)                                                                                                                                ggCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCa  <  2:281297/140‑1 (MQ=255)                                                                                                                                ggCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCa  <  1:83608/140‑1 (MQ=255)                                                                                                                                ggCGCCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCa  <  1:612850/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         GTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCA  >  NC_000913/1463825‑1464091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑