Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,515,300	A→G	Y180C (TAC→TGC)	ydcV →	putative ABC transporter membrane subunit YdcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,515,300	0	A	G	100.0%	195.8 / NA	54	Y180C (TAC→TGC)	ydcV	putative ABC transporter membrane subunit YdcV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/23);  total (31/23)

CATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCA  >  NC_000913/1515166‑1515431                                                                                                                                       |                                                                                                                                    cATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAg                                                                                                                                >  2:143642/1‑140 (MQ=255)         tcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCg                                                                                                                        >  1:584202/1‑140 (MQ=255)             tcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAAtc                                                                                                                    >  1:886575/1‑140 (MQ=255)                       ttttGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGc                                                                                                          <  1:834710/140‑1 (MQ=255)                                   gtggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCagg                                                                                              <  1:14509/140‑1 (MQ=255)                                     ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggag                                                                                            >  1:191488/1‑140 (MQ=255)                                      gtgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggagg                                                                                           >  1:12023/1‑140 (MQ=255)                                       tgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAggagga                                                                                          <  1:27861/140‑1 (MQ=255)                                         tttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAt                                                                                        >  2:604689/1‑140 (MQ=255)                                           tAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGc                                                                                      <  2:26813/140‑1 (MQ=255)                                            aaCAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt                                                                                     <  2:886575/140‑1 (MQ=255)                                            aaCAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt                                                                                     <  2:12023/140‑1 (MQ=255)                                            aaCAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt                                                                                     <  2:268456/140‑1 (MQ=255)                                                 tGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGt                                                                                >  2:141428/1‑140 (MQ=255)                                                 tGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGt                                                                                >  1:788362/1‑140 (MQ=255)                                                 tGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGt                                                                                <  1:604689/140‑1 (MQ=255)                                                 tGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGt                                                                                >  2:739563/1‑140 (MQ=255)                                                   tCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt                                                                              >  2:614823/1‑140 (MQ=255)                                                   tCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt                                                                              >  2:920016/1‑140 (MQ=255)                                                   tCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTAAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt                                                                              >  2:857896/1‑140 (MQ=255)                                                      tCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGcc                                                                           <  1:141428/140‑1 (MQ=255)                                                           cGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTc                                                                      >  1:561034/1‑140 (MQ=255)                                                            gTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCg                                                                     <  1:920016/140‑1 (MQ=255)                                                             ttttCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGt                                                                    >  1:18943/1‑140 (MQ=255)                                                             ttttCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGt                                                                    <  2:584202/140‑1 (MQ=255)                                                             ttttCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGt                                                                    >  1:347628/1‑140 (MQ=255)                                                                        cctcctGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatc                                                         <  1:854393/140‑1 (MQ=255)                                                                                    tGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGAcc                                             >  2:357745/1‑140 (MQ=255)                                                                                    tGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGAcc                                             >  1:1253/1‑140 (MQ=255)                                                                                      gTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtt                                           >  2:163301/1‑140 (MQ=255)                                                                                      gTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtt                                           >  2:164568/1‑140 (MQ=255)                                                                                      gTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtt                                           >  2:838535/1‑140 (MQ=255)                                                                                      gTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtt                                           >  1:784832/1‑140 (MQ=255)                                                                                          aGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCttt                                          >  1:380202/1‑137 (MQ=255)                                                                                          aGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCttt                                          <  2:380202/137‑1 (MQ=255)                                                                                            gCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGc                                     >  1:907662/1‑140 (MQ=255)                                                                                            gCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGc                                     <  2:907662/140‑1 (MQ=255)                                                                                                cAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAggg                                 >  2:477580/1‑139 (MQ=255)                                                                                                cAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAggg                                 >  2:611040/1‑139 (MQ=255)                                                                                                cAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGt                                 <  2:96865/140‑1 (MQ=255)                                                                                                      aTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaa                           <  2:784832/140‑1 (MQ=255)                                                                                                          tGGGGCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaa                       >  2:617896/1‑140 (MQ=255)                                                                                                             ggCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGt                    <  1:164568/140‑1 (MQ=255)                                                                                                             ggCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGCATGAACGAACGt                    >  1:556051/1‑140 (MQ=255)                                                                                                             ggCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGCATGAACGAACGt                    >  1:818451/1‑140 (MQ=255)                                                                                                              gCCAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGtt                   <  1:739563/140‑1 (MQ=255)                                                                                                               ccAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTa                  <  2:561034/140‑1 (MQ=255)                                                                                                               ccAATGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTa                  >  1:306426/1‑140 (MQ=255)                                                                                                                  aTGGCTGGCAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCg               >  1:798123/1‑140 (MQ=255)                                                                                                                   tggctggcAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGt              >  2:595305/1‑140 (MQ=255)                                                                                                                   tggctggcAAACCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGt              <  2:1253/140‑1 (MQ=255)                                                                                                                           aaaCCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTg      <  1:357745/140‑1 (MQ=255)                                                                                                                           aaaCCTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTg      <  2:18943/140‑1 (MQ=255)                                                                                                                               cTTCCGCTGCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCa  <  1:595305/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    CATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCA  >  NC_000913/1515166‑1515431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑