Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,516,041	C→T	A155V (GCC→GTC)	patD →	gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,516,041	0	C	T	100.0%	168.7 / NA	49	A155V (GCC→GTC)	patD	gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/22);  total (27/22)

TTTTCGCGGGTGCGGCGCGCTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGCCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACA  >  NC_000913/1515906‑1516172                                                                                                                                        |                                                                                                                                    ttttcgcGGGTGCGGCGCGCTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTcgcg                                                                                                                                 <  2:827005/140‑1 (MQ=255)    tcgcGGGTGCGGCGCGCTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTgg                                                                                                                              <  1:882337/140‑1 (MQ=255)                    cTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGgcgc                                                                                                              >  2:740173/1‑140 (MQ=255)                    cTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCAATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGgcgc                                                                                                              <  2:102551/140‑1 (MQ=255)                       tCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTgg                                                                                                           >  2:720928/1‑140 (MQ=255)                            aTGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCa                                                                                                      <  2:357760/140‑1 (MQ=255)                               gTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAggg                                                                                                   <  1:163664/140‑1 (MQ=255)                                 cTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGaa                                                                                                 >  1:118197/1‑140 (MQ=255)                                       gcAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGt                                                                                           <  1:720928/140‑1 (MQ=255)                                          ggTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGt                                                                                        >  1:259825/1‑140 (MQ=255)                                                  tCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAAc                                                                                >  1:63429/1‑140 (MQ=255)                                                   cTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAAcc                                                                               >  2:615533/1‑140 (MQ=255)                                                     tGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCAt                                                                             >  1:238829/1‑140 (MQ=255)                                                      gAAGGTCATGCTTCGATGATCCGTCGCGATCCGTTGGGCGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATc                                                                            <  2:769232/140‑1 (MQ=255)                                                            cATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAAt                                                                      >  1:81387/1‑140 (MQ=255)                                                             aTACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAAtt                                                                     >  1:331832/1‑140 (MQ=255)                                                                cTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTAcc                                                                  >  2:2794/1‑140 (MQ=255)                                                                    gatgatCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGc                                                              >  2:205069/1‑140 (MQ=255)                                                                     atgatCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCt                                                             <  2:81387/140‑1 (MQ=255)                                                                     atgatCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTAACCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCt                                                             <  1:62916/140‑1 (MQ=255)                                                                       gatCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGa                                                           <  1:2794/140‑1 (MQ=255)                                                                           cGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACcgc                                                       >  1:148365/1‑140 (MQ=255)                                                                           cGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACcgc                                                       >  2:153725/1‑140 (MQ=255)                                                                             tCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGt                                                     >  1:607146/1‑140 (MQ=255)                                                                                  aTCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAg                                                >  1:531048/1‑140 (MQ=255)                                                                                   tCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGt                                               >  2:101277/1‑140 (MQ=255)                                                                                           gggTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCagag                                       <  1:153725/140‑1 (MQ=255)                                                                                             gTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCt                                     >  1:251749/1‑140 (MQ=255)                                                                                             gTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCt                                     >  2:296175/1‑140 (MQ=255)                                                                                               cGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCagag                                       >  1:335505/1‑136 (MQ=255)                                                                                               cGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCagag                                       <  2:335505/136‑1 (MQ=255)                                                                                                              aCCGTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTcc                    >  2:644669/1‑140 (MQ=255)                                                                                                                 gTGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCgg                 >  1:585029/1‑140 (MQ=255)                                                                                                                  tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  1:657243/1‑140 (MQ=255)                                                                                                                  tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  2:10573/1‑140 (MQ=255)                                                                                                                  tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  2:731070/1‑140 (MQ=255)                                                                                                                  tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  2:584642/1‑140 (MQ=255)                                                                                                                  tGGAATTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGc                >  2:197039/1‑140 (MQ=255)                                                                                                                      aTTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGc            <  2:607146/140‑1 (MQ=255)                                                                                                                      aTTATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGc            <  2:251749/140‑1 (MQ=255)                                                                                                                        tATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGt          <  2:259825/140‑1 (MQ=255)                                                                                                                        tATCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGt          <  1:205069/140‑1 (MQ=255)                                                                                                                          tCCGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGa        >  2:666218/1‑140 (MQ=255)                                                                                                                           ccGCTGATGATGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGAt       <  2:657243/140‑1 (MQ=255)                                                                                                                                gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  2:120701/140‑1 (MQ=255)                                                                                                                                gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  1:615533/140‑1 (MQ=255)                                                                                                                                gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  2:118197/140‑1 (MQ=255)                                                                                                                                gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  2:238829/140‑1 (MQ=255)                                                                                                                                gatgatGGTCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTAGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACa  <  2:331832/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TTTTCGCGGGTGCGGCGCGCTGTCTGAATGGTCTGGCGGCAGGTGAATATCTTGAAGGTCATACTTCGATGATCCGTCGCGATCCGTTGGGGGTCGTGGCTTCTATCGCACCGTGGAATTATCCGCTGATGATGGCCGCGTGGAAACTTGCTCCGGCGCTGGCGGCAGGGAACTGCGTAGTGCTTAAACCATCAGAAATTACCCCGCTGACCGCGTTGAAGTTGGCAGAGCTGGCGAAAGATATCTTCCCGGCAGGCGTGATTAACA  >  NC_000913/1515906‑1516172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑